Results 31 to 40 of about 2,295,017 (227)

Bacterial genome architecture shapes global transcriptional regulation by DNA supercoiling. [PDF]

Nucleic Acids Res, 2019
DNA supercoiling acts as a global transcriptional regulator in bacteria, that plays an important role in adapting their expression programme to environmental changes, but for which no quantitative or even qualitative regulatory model is available.
El Houdaigui B, Forquet R, Hindré T, Schneider D, Nasser W, Reverchon S, Meyer S.   +6 more
europepmc   +2 more sources

DNA supercoiling inhibits DNA knotting [PDF]

Nucleic Acids Research, 2008
Despite the fact that in living cells DNA molecules are long and highly crowded, they are rarely knotted. DNA knotting interferes with the normal functioning of the DNA and, therefore, molecular mechanisms evolved that maintain the knotting and catenation level below that which would be achieved if the DNA segments could pass randomly through each ...
Burnier Y, Dorier J, Stasiak A
openaire   +4 more sources

Mitochondrial stress in advanced fibrosis and cirrhosis associated with chronic hepatitis B, chronic hepatitis C, or nonalcoholic steatohepatitis

Hepatology, EarlyView., 2022
Adaptive mitochondrial mechanisms allow mitochondrial resilience and prevent the worsening of fibrosis, while deregulation of these mechanisms promotes the progression from no/minimal‐mild (F0‐F2) fibrosis to advanced fibrosis and cirrhosis (F3‐F4). Abstract Background and Aims Hepatitis B virus (HBV) infection causes oxidative stress (OS) and alters ...
Dimitri Loureiro, Issam Tout, Stéphanie Narguet, Cheikh Mohamed Bed, Morgane Roinard, Ahmad Sleiman, Nathalie Boyer, Nathalie Pons‐Kerjean, Corinne Castelnau, Nathalie Giuly, Dorothy Tonui, Vassili Soumelis, Jamel El Benna, Patrick Soussan, Richard Moreau, Valérie Paradis, Abdellah Mansouri, Tarik Asselah   +17 more
wiley   +1 more source

A spatially resolved stochastic model reveals the role of supercoiling in transcription regulation.

PLoS Computational Biology, 2022
In Escherichia coli, translocation of RNA polymerase (RNAP) during transcription introduces supercoiling to DNA, which influences the initiation and elongation behaviors of RNAP.
Yuncong Geng, Christopher Herrick Bohrer, Nicolás Yehya, Hunter Hendrix, Lior Shachaf, Jian Liu, Jie Xiao, Elijah Roberts   +7 more
doaj   +1 more source

The balance between gyrase and topoisomerase I activities determines levels of supercoiling, nucleoid compaction, and viability in bacteria

Frontiers in Microbiology, 2023
Two enzymes are responsible for maintaining supercoiling in the human pathogen Streptococcus pneumoniae, gyrase (GyrA2GyrB2) and topoisomerase I. To attain diverse levels of topoisomerase I (TopoI, encoded by topA), two isogenic strains derived from wild-
Míriam García-López, Diego Megias, María-José Ferrándiz, Adela G. de la Campa, Adela G. de la Campa   +4 more
doaj   +1 more source

Interaction between transcribing RNA polymerase and topoisomerase I prevents R-loop formation in E. coli

Nature Communications, 2022
In E. coli, disruption of TopoI and RNAP interaction decreases cells viability and leads to hypernegative DNA supercoiling and R loops accumulation. TopoI and DNA gyrase bind around transcription units and TopoI recognizes cleavage sites by a specific ...
Dmitry Sutormin, Alina Galivondzhyan, Olga Musharova, Dmitrii Travin, Anastasiia Rusanova, Kseniya Obraztsova, Sergei Borukhov, Konstantin Severinov   +7 more
doaj   +1 more source

Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]

, 2020
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio, Bryant, Zev, Cofsky, Joshua C, Doudna, Jennifer A, Ivanov, Ivan E, Wright, Addison V   +5 more
core   +3 more sources

Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters

mSystems, 2021
DNA supercoiling acts as a global transcriptional regulator that contributes to the rapid transcriptional response of bacteria to many environmental changes.
Raphaël Forquet, Maïwenn Pineau, William Nasser, Sylvie Reverchon, Sam Meyer   +4 more
doaj   +1 more source

Divergent RNA transcription:A role in promoter unwinding? [PDF]

, 2013
New approaches using biotinylated-psoralen as a probe for investigating DNA structure have revealed new insights into the relationship between DNA supercoiling, transcription and chromatin compaction.
Corless, Samuel, Gilbert, Nick, Naughton, Catherine   +2 more
core   +1 more source

Towards Conformation-Sensitive Inhibition of Gyrase: Implications of Mechanistic Insight for the Identification and Improvement of Inhibitors

Molecules, 2021
Gyrase is a bacterial type IIA topoisomerase that catalyzes negative supercoiling of DNA. The enzyme is essential in bacteria and is a validated drug target in the treatment of bacterial infections.
Dagmar Klostermeier
doaj   +1 more source