Results 51 to 60 of about 1,452,122 (130)
Biology, 2021 DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined.
Jorge Cebrián +5 more
doaj +1 more source
Advanced Science, EarlyView.This study characterizes MfAgo, an enzyme exhibiting highly efficient DNA‐guided DNA and RNA cleavage capability. Precise RNA cleavage strictly requires dimerization to stabilize the PIWI‐loop region, while its robust DNA cleavage functions in a dimerization‐independent manner.
Fuyong Huang +9 more
wiley +1 more source
Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing
Cell Reports, 2021 Summary: Accumulation of topological stress in the form of DNA supercoiling is inherent to the advance of RNA polymerase II (Pol II) and needs to be resolved by DNA topoisomerases to sustain productive transcriptional elongation.
Andrés Herrero-Ruiz +6 more
doaj +1 more source
Thermal Shifts Dominate Single‐Gene Responses to Transcription‐Targeting Antibiotics
Advanced Science, EarlyView.Thermal shifts reshape bacterial responses to transcription‐targeting antibiotics. In E. coli, temperature can dominate antibiotic‐induced genome‐wide expression, particularly outside 30°C–39°C, through changes in adenosine triphosphate (ATP), nucleoid organization, and drug‐target engagement.
Rahul Jagadeesan +5 more
wiley +1 more source
The bacterial RNA polymerase-associated CarD protein couples promoter activity to DNA supercoiling
Nature CommunicationsRNA polymerase (RNAP) must unwind duplex DNA prior to transcription initiation. In bacteria, unwinding starts at a DNA motif called the promoter −10 element. Specifically, non-template strand bases interact with RNAP to trigger the process.
David Forrest +2 more
doaj +1 more source
Microorganisms, 2019 Prokaryotes have an essential gene—gyrase—that catalyzes negative supercoiling of plasmid and chromosomal DNA. Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic ...
Nikolay S. Rovinskiy +3 more
doaj +1 more source
ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes
Nature Communications, 2023 The function of the mitogen-activated protein kinase signaling pathway is required for the activation of immediate early genes (IEGs), including EGR1 and FOS, for cell growth and proliferation.
Heeyoun Bunch +10 more
doaj +1 more source
Studies of DNA supercoiling in vivo and in vitro [PDF]
, 1990 This thesis describes a number of diverse experiments whose common theme is to elaborate some aspect of DNA supercoiling. The torsion elastic constant of DNA is measure as a function of superhelix density using the technique of picosecond Time Resolved ...
Cook, D. N.
core +2 more sources
Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system
Nature Communications, 2023 CRISPR-Cas effector complexes enable the defense against foreign nucleic acids and have recently been exploited as molecular tools for precise genome editing at a target locus. To bind and cleave their target, the CRISPR-Cas effectors have to interrogate
Pierre Aldag +8 more
doaj +1 more source
Modulation of Epigenetic Control by Advanced Molecular Tweezers
Chemistry – A European Journal, EarlyView.Tailored dimeric molecular tweezers recognize the DNA‐binding part of Histone H4 and interrupt the essential histone‐DNA interaction. ABSTRACT This work addresses the question if supramolecular systems can be tailored to interfere with posttranslational modifications (PTM) and epigenetics.
My‐Hue‐Le +9 more
wiley +1 more source