Emergence of supercoiling-mediated regulatory networks through the evolution of bacterial chromosome organization. [PDF]
PLoS Computational BiologyDNA supercoiling-the level of twisting and writhing of the DNA molecule around itself-plays an important role in the regulation of gene expression in bacteria by modulating promoter activity.
Théotime Grohens +2 more
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The bacterial RNA polymerase-associated CarD protein couples promoter activity to DNA supercoiling [PDF]
Nature CommunicationsRNA polymerase (RNAP) must unwind duplex DNA prior to transcription initiation. In bacteria, unwinding starts at a DNA motif called the promoter −10 element. Specifically, non-template strand bases interact with RNAP to trigger the process.
David Forrest +2 more
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Cinnamic‐Hydroxamic‐Acid Derivatives Exhibit Antibiotic, Anti‐Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid‐Associated Protein HU [PDF]
Advanced ScienceFinding novel compounds and drug targets is crucial for antibiotic development. The nucleoid‐associated protein HU plays a significant role in bacterial DNA metabolism, supercoiling, and biofilm formation, making it a promising new target.
Huan Chen +22 more
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DNA supercoiling-mediated G4/R-loop formation tunes transcription by controlling the access of RNA polymerase [PDF]
Nature CommunicationsRNA polymerase (RNAP) is a processive motor that modulates DNA supercoiling and reshapes DNA structures. The feedback loop between the DNA topology and transcription remains elusive.
Jihee Hwang +8 more
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Supercoiling DNA optically [PDF]
Proceedings of the National Academy of Sciences, 2019 AbstractCellular DNA is regularly subject to torsional stress during genomic processes, such as transcription and replication, resulting in a range of supercoiled DNA structures.1,2,3 For this reason, methods to prepare and study supercoiled DNA at the single-molecule level are widely used, including magnetic,4,5,6 angular-optical,7,8,9 micro-pipette ...
King, Graeme A. +3 more
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Frontiers in Microbiology, 2023 Two enzymes are responsible for maintaining supercoiling in the human pathogen Streptococcus pneumoniae, gyrase (GyrA2GyrB2) and topoisomerase I. To attain diverse levels of topoisomerase I (TopoI, encoded by topA), two isogenic strains derived from wild-
Míriam García-López +4 more
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Supercoiling and looping promote DNA base accessibility and coordination among distant sites
Nature Communications, 2021 DNA supercoiling can result in underwinding with negative supercoiling or overwinding with positive supercoiling of the DNA double helix. Here the authors reveal insights into the dynamic relationship between DNA supercoiling-induced sequence-dependent ...
Jonathan M. Fogg +4 more
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mSystems, 2021 Bacterial gene expression is controlled at multiple levels, with chromosome supercoiling being one of the most global regulators. Global DNA supercoiling is maintained by the orchestrated action of topoisomerases.
Martyna Gongerowska-Jac +8 more
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FEDS: a Novel Fluorescence-Based High-Throughput Method for Measuring DNA Supercoiling
mBio, 2020 DNA supercoiling (DS) is essential for life because it controls critical processes, including transcription, replication, and recombination. Current methods to measure DNA supercoiling in vivo are laborious and unable to examine single cells.
Alexandre Duprey, Eduardo A. Groisman
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A spatially resolved stochastic model reveals the role of supercoiling in transcription regulation.
PLoS Computational Biology, 2022 In Escherichia coli, translocation of RNA polymerase (RNAP) during transcription introduces supercoiling to DNA, which influences the initiation and elongation behaviors of RNAP.
Yuncong Geng +7 more
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