Results 11 to 20 of about 15,229 (210)
Supercoiling in DNA and chromatin [PDF]
Current Opinion in Genetics & Development, 2014 Supercoiling is a fundamental property of DNA and chromatin. It is modulated by polymerase and topoisomerase activities and, through regulated constraint, by DNA/chromatin binding proteins. As a non-covalent and elusive topological modification, supercoiling has proved intractable to research despite being a crucial regulator of nuclear structure and ...
Gilbert, N., Allan, J.
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Supercoiling DNA optically [PDF]
Proceedings of the National Academy of Sciences, 2019 Abstract Cellular DNA is regularly subject to torsional stress during genomic processes, such as transcription and replication, resulting in a range of supercoiled DNA structures. 1,2,3 For this reason, methods to prepare and study supercoiled DNA at the single-molecule level are ...
King, Graeme A. +3 more
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Supercoiling DNA Locates Mismatches [PDF]
Physical Review Letters, 2017 We present a method of detecting sequence defects by supercoiling DNA with magnetic tweezers. The method is sensitive to a single mismatched base pair in a DNA sequence of several thousand base pairs. We systematically compare DNA molecules with 0 to 16 adjacent mismatches at 1 M monovalent salt ...
Dittmore, Andrew +4 more
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Profiling DNA supercoiling domains in vivo [PDF]
Genomics Data, 2014 Transitions in DNA structure have the capacity to regulate genes, but have been poorly characterised in eukaryotes due to a lack of appropriate techniques.
Samuel Corless +2 more
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Physics of Fluids, 2014 Supercoiling is the large-scale secondary coiling or buckling of a structure that is already coiled at a smaller scale. Here, we show experimentally that a fluid-mechanical analog of supercoiling can occur when a thin “rope” of viscous fluid falls vertically from a great height onto a surface. For appropriate values of the viscosity ν, the flow rate Q,
Habibi, Mehdi +3 more
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Counterintuitive DNA Sequence Dependence in Supercoiling-Induced DNA Melting.
PLoS ONE, 2015 The metabolism of DNA in cells relies on the balance between hybridized double-stranded DNA (dsDNA) and local de-hybridized regions of ssDNA that provide access to binding proteins.
Rifka Vlijm, Jaco V D Torre, Cees Dekker
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Chromosomal domains of supercoiling in Salmonella typhimurium
, 1993 The chromosomes of enteric bacteria are divided into about 50 independently supercoiled domains. It is not known whether the net level of DNA supercoiling is similar in each domain, or whether the domains are differentially supercoiled. We have addressed
Pavitt, Graham D.; id_orcid +1 more
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Role of DNA supercoiling in genome structure and regulation [PDF]
, 2014 A principle challenge of modern biology is to understand how the human genome is organised and regulated within a nucleus. The field of chromatin biology has made significant progress in characterising how protein and DNA modifications reflect ...
Corless, Samuel
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Behavior of Supercoiled DNA [PDF]
Biophysical Journal, 1998 We study DNA supercoiling in a quantitative fashion by micromanipulating single linear DNA molecules with a magnetic field gradient. By anchoring one end of the DNA to multiple sites on a magnetic bead and the other end to multiple sites on a glass surface, we were able to exert torsional control on the DNA. A rotating magnetic field was used to induce
Strick, T.R. +3 more
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Data in Brief, 2020 In enteric bacteria, DNA supercoiling is highly responsive to environmental conditions. Host specific features of environment serve as cues for the expression of genes required for colonization of host niches via changing supercoiling [1].
Natalia E. Gogoleva +4 more
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