Results 21 to 30 of about 22,006 (218)

DNA Supercoiling Regulates the Motility of Campylobacter jejuni and Is Altered by Growth in the Presence of Chicken Mucus

mBio, 2016
Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans, but relatively little is known about the global regulation of virulence factors during infection of chickens or humans.
Claire Shortt, Eoin Scanlan, Amber Hilliard, Chiara E. Cotroneo, Billy Bourke, Tadhg Ó Cróinín   +5 more
doaj   +1 more source

Getting DNA twist rigidity from single molecule experiments [PDF]

, 2004
We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we get an estimation of ...
A. V. Vologodskii, C. Bouchiat, D. M. Stump, D. M. Stump, E. Doedel, J. Aldinger, O. Kratky, P. J. Hagerman, Sébastien Neukirch, T. R. Strick, V. V. Rybenkov   +10 more
core   +3 more sources

Women’s contribution in understanding how topoisomerases, supercoiling, and transcription control genome organization

Frontiers in Molecular Biosciences, 2023
One of the biggest paradoxes in biology is that human genome is roughly 2 m long, while the nucleus containing it is almost one million times smaller.
Laura Martin, Maria Victoria Neguembor, Maria Victoria Neguembor, Maria Pia Cosma, Maria Pia Cosma, Maria Pia Cosma, Maria Pia Cosma, Maria Pia Cosma   +7 more
doaj   +1 more source

Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates

Biology, 2021
DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined.
Jorge Cebrián, Victor Martínez, Pablo Hernández, Dora B. Krimer, María-José Fernández-Nestosa, Jorge B. Schvartzman   +5 more
doaj   +1 more source

A Crystal Structure of the Bifunctional Antibiotic Simocyclinone D8, Bound to DNA Gyrase [PDF]

, 2009
Simocyclinones are bifunctional antibiotics that inhibit bacterial DNA gyrase by preventing DNA binding to the enzyme. We report the crystal structure of the complex formed between the N-terminal domain of the Escherichia coli gyrase A subunit and ...
A. Maxwell, A. R. McKay, C. E.M. Stevenson, D. M. Lawson, Flatman, Flatman, Galm, H.-P. Fiedler, L. A. Mitchenall, Laponogov, Lewis, Li, Livermore, M. J. Buttner, M. J. Edwards, Maxwell, Maxwell, Morais Cabral, Oliphant, R. H. Flatman, Ruiz, Schimana, Schoeffler, T. A. Clarke, T. B.K. Le, Theobald, Trefzer, Tsai, Yoshida, Yoshida   +29 more
core   +1 more source

Transcriptional Response of Streptomyces coelicolor to Rapid Chromosome Relaxation or Long-Term Supercoiling Imbalance

Frontiers in Microbiology, 2019
Negative DNA supercoiling allows chromosome condensation and facilitates DNA unwinding, which is required for the occurrence of DNA transaction processes, i.e., DNA replication, transcription and recombination.
Marcin Jan Szafran, Martyna Gongerowska, Tomasz Małecki, Marie Elliot, Dagmara Jakimowicz   +4 more
doaj   +1 more source

Single-nucleotide resolution detection of Topo IV cleavage activity in the Escherichia coli genome with Topo-Seq

Frontiers in Microbiology, 2023
Topoisomerase IV (Topo IV) is the main decatenation enzyme in Escherichia coli; it removes catenation links that are formed during DNA replication. Topo IV binding and cleavage sites were previously identified in the E.
Dmitry Sutormin, Alina Galivondzhyan, Azamat Gafurov, Konstantin Severinov   +3 more
doaj   +1 more source

Stress Sensors and Signal Transducers in Cyanobacteria

Sensors, 2010
In living cells, the perception of environmental stress and the subsequent transduction of stress signals are primary events in the acclimation to changes in the environment.
Sergey Kryazhov, Kirill Mironov, Maria Sinetova, Anna Zorina, Dmitry A. Los, Vladislav V. Zinchenko   +5 more
doaj   +1 more source

Interaction between transcribing RNA polymerase and topoisomerase I prevents R-loop formation in E. coli

Nature Communications, 2022
In E. coli, disruption of TopoI and RNAP interaction decreases cells viability and leads to hypernegative DNA supercoiling and R loops accumulation. TopoI and DNA gyrase bind around transcription units and TopoI recognizes cleavage sites by a specific ...
Dmitry Sutormin, Alina Galivondzhyan, Olga Musharova, Dmitrii Travin, Anastasiia Rusanova, Kseniya Obraztsova, Sergei Borukhov, Konstantin Severinov   +7 more
doaj   +1 more source

A role for non-B DNA forming sequences in mediating microlesions causing human inherited disease [PDF]

, 2015
Missense/nonsense mutations and micro-deletions/micro-insertions of
Aguilera, Bacolla, Bacolla, Bacolla, Bacolla, Bacolla, Ball, Belotserkovskii, Bharti, Biffi, Bochman, Boyer, Brooks, Bzymek, Cebrián, Chen, Chen, Chuzhanova, Clark, Cooper, Cooper, Cooper, De, Ding, Dong, Du, Fleming, Greenblatt, Guo, Gusev, Iyer, Jain, Jain, Javadekar, Jeitany, Kaushik Tiwari, Kondrashov, Kouzine, Kregten, Kunkel, Kunkel, Kunkel, Lam, Lee, Levinson, León-Ortiz, Lopes, Lovett, Lu, Majumdar, Margolin, Morikawa, Mort, Mukherjee, Mukherjee, Murat, Nambiar, Patel, Raghavan, Reeves, Rosche, Rouleau, Sanders, Schofield, Sen, Sharma, Sinden, Sinden, Spurlock, Stenson, Stenson, Tappino, Vasquez, Verdin, Wang, Wells, Wickramasinghe, Williams, Wu, Wu, Wu, Wu, Yang, You, Zhao, Zhao, Zhou   +86 more
core   +1 more source