Results 181 to 190 of about 7,352 (214)
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Thermostability and superhelicity of plasmid DNA in Bacillus stearothermophilus

Molecular and General Genetics MGG, 1987
The thermostability of the staphylococcal plasmids pC194 and pUB110 and their antibiotic-resistance determinants was examined upon transfer to Bacillus stearothermophilus CU21. Plasmid pGS13, a pUB110 derivative carrying the chloramphenicol acetyltransferase (CAT) gene of pC194, could be maintained up to the maximum growth temperature (68 degrees C) by
E, Soutschek-Bauer   +3 more
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Theory of DNA Superhelicity

1985
When a duplex DNA molecule is covalently closed into a circle, conservation of the antiparallel chemical (5′–3′) orientations of the two strands dictates that the resulting ring molecule must have the topological structure of two closed, interlinked circles. This fixes the value of the molecular linking number Lk.
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Structures of Homopurine-homopyrimidine Tract in Superhelical DNA

Journal of Biomolecular Structure and Dynamics, 1986
For homopurine-homopyrimidine tracts in superhelical DNA, we propose a structure involving Watson-Crick and Hoogsteen paired triple helixes, hairpin loops and unstructured domains. Topologically, the whole structure is equivalent to an open region. The proposed structure is consistent with available S1 cleavage, pH and alkylation data and energetics ...
V I, Lyamichev   +2 more
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Superhelical Escherichia coli DNA: Relaxation by coumermycin

Journal of Molecular Biology, 1978
Abstract Folded chromosomes isolated from Escherichia coli strains after treatment with coumermycin A 1 in vivo , an inhibitor of DNA gyrase (Gellert et al. , 1976 a,b ), were found to have reduced DNA superhelical densities. This loss of DNA supercoiling paralleled inhibition of DNA synthesis.
K, Drlica, M, Snyder
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Attachment of nascent RNA molecules to superhelical DNA

Journal of Molecular Biology, 1975
Comparisons are made of the attachment of nascent RNA molecules synthesized in vitro with Escherichia coli RNA polymerase on native bacteriophage PM2 DNA, a double-helical circular DNA with −110 superhelical twists, and on a derivative of this DNA with no superhelical twists.
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Superhelical model of DNA for regulation of gene expression

Journal of Theoretical Biology, 1983
A superhelical higher-order structure is proposed as a model of template DNA for regulation of transcription in simian virus 40. A stem-and-loop structure is located 3' downstream from the transcriptional initiation site in the sense strand. The complementary sequences to the loop regions in both strands are found between the 70th and the 300th ...
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Energetics of the strand separation transition in superhelical DNA

Journal of Molecular Biology, 1992
In this paper the values of three free energy parameters governing the superhelical strand separation transition are determined by analysis of available experimental data. These are the free energy, a, needed to initiate a run of separation, the torsional stiffness, C, associated with interstrand winding of the two single strands comprising a separated
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Superhelical DNA in yeast chromosomes

Experimental Cell Research, 1977
S J, McCready, B S, Cox, C S, McLaughlin
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Superhelical DNA

Trends in Biochemical Sciences, 1980
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