Results 21 to 30 of about 2,039,836 (177)

Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]

, 2020
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio, Bryant, Zev, Cofsky, Joshua C, Doudna, Jennifer A, Ivanov, Ivan E, Wright, Addison V   +5 more
core   +3 more sources

S1-Type Endonuclease 2 in Dedifferentiating Arabidopsis Protoplasts: Translocation to the Nucleus in Senescing Protoplasts Is Associated with De-Glycosylation. [PDF]

PLoS ONE, 2017
Cell dedifferentiation characterizes the transition of leaf cells to protoplasts and is accompanied by global chromatin decondensation. Here we show that in Arabidopsis, chromocentric chromatin undergoes prompt and gradual decondensation upon ...
Yemima Givaty-Rapp, Narendra Singh Yadav, Asif Khan, Gideon Grafi   +3 more
doaj   +1 more source

Interactions of Cisplatin and Daunorubicin at the chromatin level [PDF]

, 2020
Unexpectedly, the widely used anticancer agents Cisplatin (Cis-Pt) and Daunorubicin (Dauno) exhibited cell type- and concentration-dependent synergy or antagonism in vitro.
Bacsó, Zsolt, Firouzi Niaki, Erfaneh, Imre, László, Nánási, Péter Jr., Szabó, Gábor, Tarapcsák, Szabolcs, Van Acker, Thibaut, Vanhaecke, Frank   +7 more
core   +2 more sources

Helical antimicrobial peptides assemble into protofibril scaffolds that present ordered dsDNA to TLR9

Nature Communications, 2019
Amphihelical antimicrobial peptides (AMPs) are bactericidal host defense factors, but their function as immunomodulators is emerging. Here the authors show that several AMPs organize DNA into periodic nanocrystals by self-assembling into superhelical ...
Ernest Y. Lee, Changsheng Zhang, Jeremy Di Domizio, Fan Jin, Will Connell, Mandy Hung, Nicolas Malkoff, Veronica Veksler, Michel Gilliet, Pengyu Ren, Gerard C. L. Wong   +10 more
doaj   +1 more source

Polymer induced condensation of dna supercoils [PDF]

, 2008
Macromolecular crowding is thought to be a significant factor driving DNA condensation in prokaryotic cells. Whereas DNA in prokaryotes is supercoiled, studies on crowding-induced DNA condensation have so far focused on linear DNA.
Bessa Ramos Jr., J.E., Ruggiero Neto, J., Vries, R.J., de   +2 more
core   +2 more sources

Snf2 family ATPases and DExx box helicases:differences and unifying concepts from high-resolution crystal structures [PDF]

, 2006
Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA–protein structures including chromatin. Snf2 family enzymes are related in sequence to DExx box helicases,
Aasland, Ahne, Akhtar, Andrew Flaus, Asturias, Auble, Auble, Auble, Bazett-Jones, Becker, Beerens, Bouazoune, Boyer, Boyer, Briggs, Caruthers, Corona, Cote, Dasgupta, Davis, Deaconescu, Dhalluin, Dumont, Durr, Eisen, Fan, Fitzgerald, Flaus, Flaus, Goldmark, Gorbalenya, Grune, Harald Dürr, Havas, Hishida, Ito, Jaskelioff, Kagalwala, Karl-Peter Hopfner, Kawaoka, Kim, Laurent, Laurent, Levin, Levin, Lia, Mahdi, Mannhaupt, Mizuguchi, Morillon, Myong, Peterson, Petukhova, Petukhova, Phelan, Pray-Grant, Quinn, Richmond, Ristic, Saha, Saha, Schild, Sengoku, Serebrov, Shen, Shinohara, Simic, Sims, Singleton, Singleton, Singleton, Smith, Soultanas, Sprouse, Stanley, Studitsky, Subramanya, Sukhodolets, Svejstrup, Swagemakers, Tai, Thoma, Tom Owen-Hughes, Tran, Tsukiyama, van den Boom, van Gool, Van Komen, Velankar, Wang, Whitehouse, Woudstra, Yu, Yu, Zhang, Zofall   +95 more
core   +4 more sources

Chromatin remodeller SMARCA4 recruits topoisomerase 1 and suppresses transcription-associated genomic instability

Nature Communications, 2016
Topoisomerase 1 (TOP1) relieves superhelical tension when DNA strands are unwound during transcription. Here, Husain et al. report that SMARCA4, an ATP-dependent chromatin remodeller, is associated with TOP1 and suppresses transcription-associated ...
Afzal Husain, Nasim A. Begum, Takako Taniguchi, Hisaaki Taniguchi, Maki Kobayashi, Tasuku Honjo   +5 more
doaj   +1 more source

Application of Plasmid Engineering to Enhance Yield and Quality of Plasmid for Vaccine and Gene Therapy

Bioengineering, 2019
There is an increased interest in plasmid DNA as therapeutics. This is evident in the number of ongoing clinical trials involving the use of plasmid DNA.
Olusegun Folarin, Darren Nesbeth, John M. Ward, Eli Keshavarz-Moore   +3 more
doaj   +1 more source

Structural Coupling between RNA Polymerase Composition and DNA Supercoiling in Coordinating Transcription: a Global Role for the Omega Subunit?

mBio, 2011
In growing bacterial cells, the global reorganization of transcription is associated with alterations of RNA polymerase composition and the superhelical density of the DNA.
Marcel Geertz, Andrew Travers, Sanja Mehandziska, Patrick Sobetzko, Sarath Chandra Janga, Nobuo Shimamoto, Georgi Muskhelishvili   +6 more
doaj   +1 more source

Correction: Free energy profiles for unwrapping the outer superhelical turn of nucleosomal DNA. [PDF]

PLoS Computational Biology, 2019
[This corrects the article DOI: 10.1371/journal.pcbi.1006024.].
Hidetoshi Kono, Shun Sakuraba, Hisashi Ishida   +2 more
doaj   +1 more source