Results 41 to 50 of about 7,352 (214)
Snf2 family ATPases and DExx box helicases:differences and unifying concepts from high-resolution crystal structures [PDF]
, 2006 Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA-protein structures including chromatin. Snf2 family enzymes are related in sequence to DExx box helicases,
Owen-Hughes, Tom; id_orcid +3 more
core +1 more source
An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
BioTechniques, 2009 A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study.
Marita C. Barth +2 more
doaj +1 more source
Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations
eLife, 2020 Chromatin remodeling plays important roles in gene regulation during development, differentiation and in disease. The chromatin remodeling enzyme CHD4 is a component of the NuRD and ChAHP complexes that are involved in gene repression.
Lucas Farnung +2 more
doaj +1 more source
Molecular dynamics simulations of DNA-free and DNA-bound TAL effectors. [PDF]
PLoS ONE, 2013 TAL (transcriptional activator-like) effectors (TALEs) are DNA-binding proteins, containing a modular central domain that recognizes specific DNA sequences. Recently, the crystallographic studies of TALEs revealed the structure of DNA-recognition domain.
Hua Wan +4 more
doaj +1 more source
Padlock oligonucleotides as a tool for labeling superhelical DNA [PDF]
Nucleic Acids Research, 2002 Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids.
Roulon, T. +5 more
openaire +2 more sources
DNAIntroduction: Does DNA superhelicity effect nucleic acid interactions with drugs? To test such a possibility, the interactions of the linear and superhelical forms of the pGEX-4T-2 plasmid have been investigated with a newly synthesized compound, 4 ...
Dominika Janiszek +7 more
doaj +1 more source
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation. [PDF]
PLoS Computational Biology, 2016 The ability to precisely modify genomes and regulate specific genes will greatly accelerate several medical and engineering applications. The CRISPR/Cas9 (Type II) system binds and cuts DNA using guide RNAs, though the variables that control its on ...
Iman Farasat, Howard M Salis
doaj +1 more source
Circadian rhythms of superhelical status of DNA in cyanobacteria [PDF]
Proceedings of the National Academy of Sciences, 2007 The cyanobacterium Synechococcus elongatus expresses robust circadian (daily) rhythms under the control of the KaiABC-based core clockwork. Unlike eukaryotic circadian systems characterized thus far, the cyanobacterial clockwork modulates gene expression patterns globally and specific clock gene promoters are not ...
Mark A, Woelfle +3 more
openaire +2 more sources
Transcription of Superhelical DNA from Cell Nuclei [PDF]
European Journal of Biochemistry, 1977 Structures resembling nuclei may be released by gently lysing human or frog cells in solutions containing non‐ionic detergents and 1.95 M NaCl. These structures, or nucleoids, sediment in sucrose gradients containing intercalating agents in the manner characteristic of DNA that is intact, supercoiled and circular.
Colman, A, Cook, P
openaire +3 more sources
Preferentially quantized linker DNA lengths in Saccharomyces cerevisiae.
PLoS Computational Biology, 2008 The exact lengths of linker DNAs connecting adjacent nucleosomes specify the intrinsic three-dimensional structures of eukaryotic chromatin fibers. Some studies suggest that linker DNA lengths preferentially occur at certain quantized values, differing ...
Ji-Ping Wang +5 more
doaj +1 more source