Results 31 to 40 of about 7,436 (214)
The B – A transition in superhelical DNA
Nucleic Acids Research, 1990 Relaxation of a DNA superhelical stress due to the B to A transition induced by trifluoroethanol has been studied by assessing the change of DNA orientation in a flow gradient. Using DNAs of different superhelical densities, a decrease in the winding angle during the B----A shift of DNA was found to be 1.5 degrees per base pair in solution.
, Krylov DYu, V L, Makarov, V I, Ivanov
openaire +3 more sources
Superhelical DNA of polytene chromosomes Chironomus thummi [PDF]
, 1988 Предложен микровариант флюоресцентного метода измерения степени суперспиральности 0 ДНК в растворе, гелях и хромосомах. Измерения а ДНК депротеинизированных политенных хромосом свидетельствуют, что на один нуклеосомный повтор приходится 1,84÷1,88 витка ...
Груздев, А.Д. +1 more
core +3 more sources
mBio, 2011 In growing bacterial cells, the global reorganization of transcription is associated with alterations of RNA polymerase composition and the superhelical density of the DNA.
Marcel Geertz +6 more
doaj +1 more source
eLife, 2019 Transcription activator-like effectors (TALEs) bind DNA through an array of tandem 34-residue repeats. How TALE repeat domains wrap around DNA, often extending more than 1.5 helical turns, without using external energy is not well understood.
Kathryn Geiger-Schuller +3 more
doaj +1 more source
Snf2 family ATPases and DExx box helicases:differences and unifying concepts from high-resolution crystal structures [PDF]
, 2006 Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA-protein structures including chromatin. Snf2 family enzymes are related in sequence to DExx box helicases,
Owen-Hughes, Tom; id_orcid +3 more
core +1 more source
An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
BioTechniques, 2009 A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study.
Marita C. Barth +2 more
doaj +1 more source
Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations
eLife, 2020 Chromatin remodeling plays important roles in gene regulation during development, differentiation and in disease. The chromatin remodeling enzyme CHD4 is a component of the NuRD and ChAHP complexes that are involved in gene repression.
Lucas Farnung +2 more
doaj +1 more source
Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA
Biomolecules, 2021 E. coli histone-like protein HU has been shown to interact with different topological forms of DNA. Using radiolabeled HU, we examine the effects of DNA supercoiling on HU–DNA interactions.
Li Huang, Zhenfeng Zhang, Roger McMacken
doaj +1 more source
Padlock oligonucleotides as a tool for labeling superhelical DNA [PDF]
Nucleic Acids Research, 2002 Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids.
Roulon, T. +5 more
openaire +2 more sources
H-NS forms a superhelical protein scaffold for DNA condensation [PDF]
, 2010 The histone-like nucleoid structuring (H-NS) protein plays a fundamental role in DNA condensation and is a key regulator of enterobacterial gene expression in response to changes in osmolarity, pH, and temperature.
Stefan T. Arold +3 more
core +1 more source