Results 31 to 40 of about 11,463 (183)
The B – A transition in superhelical DNA
Nucleic Acids Research, 1990 Relaxation of a DNA superhelical stress due to the B to A transition induced by trifluoroethanol has been studied by assessing the change of DNA orientation in a flow gradient. Using DNAs of different superhelical densities, a decrease in the winding angle during the B----A shift of DNA was found to be 1.5 degrees per base pair in solution.
, Krylov DYu, V L, Makarov, V I, Ivanov
openaire +3 more sources
eLife, 2019 Transcription activator-like effectors (TALEs) bind DNA through an array of tandem 34-residue repeats. How TALE repeat domains wrap around DNA, often extending more than 1.5 helical turns, without using external energy is not well understood.
Kathryn Geiger-Schuller +3 more
doaj +1 more source
The main early and late promoters of Bacillus subtilis phage ø29 form unstable open complexes with sA-RNA polymerase that are stabilized by DNA supercoiling [PDF]
, 1993 Most Escherichia coli promoters studied so far form stable open complexes with σ70-RNA polymerase which have relatively long half-lives and, therefore, are resistant to a competitor challenge. A few exceptions are nevertheless known.
Rojo, Fernando +3 more
core +1 more source
Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations
eLife, 2020 Chromatin remodeling plays important roles in gene regulation during development, differentiation and in disease. The chromatin remodeling enzyme CHD4 is a component of the NuRD and ChAHP complexes that are involved in gene repression.
Lucas Farnung +2 more
doaj +1 more source
Molecular dynamics simulations of DNA-free and DNA-bound TAL effectors. [PDF]
PLoS ONE, 2013 TAL (transcriptional activator-like) effectors (TALEs) are DNA-binding proteins, containing a modular central domain that recognizes specific DNA sequences. Recently, the crystallographic studies of TALEs revealed the structure of DNA-recognition domain.
Hua Wan +4 more
doaj +1 more source
The size of the nucleosome [PDF]
, 2011 The structural origin of the size of the 11 nm nucleosomal disc is addressed. On the nanometer length-scale the organization of DNA as chromatin in the chromosomes involves a coiling of DNA around the histone core of the nucleosome.
A. Travers +3 more
core +2 more sources
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation. [PDF]
PLoS Computational Biology, 2016 The ability to precisely modify genomes and regulate specific genes will greatly accelerate several medical and engineering applications. The CRISPR/Cas9 (Type II) system binds and cuts DNA using guide RNAs, though the variables that control its on ...
Iman Farasat, Howard M Salis
doaj +1 more source
A helicoidal transfer matrix model for inhomogeneous DNA melting [PDF]
, 2006 An inhomogeneous helicoidal nearest-neighbor model with continuous degrees of freedom is shown to predict the same DNA melting properties as traditional long-range Ising models, for free DNA molecules in solution, as well as superhelically stressed DNA ...
D. Poland +3 more
core +2 more sources
Mechanical response of plectonemic DNA: an analytical solution
, 2008 We consider an elastic rod model for twisted DNA in the plectonemic regime. The molecule is treated as an impenetrable tube with an effective, adjustable radius.
Allemand J. F. +31 more
core +2 more sources
Padlock oligonucleotides as a tool for labeling superhelical DNA [PDF]
Nucleic Acids Research, 2002 Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids.
Roulon, T. +5 more
openaire +2 more sources