Results 31 to 40 of about 2,039,836 (177)
Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA
Biomolecules, 2021 E. coli histone-like protein HU has been shown to interact with different topological forms of DNA. Using radiolabeled HU, we examine the effects of DNA supercoiling on HU–DNA interactions.
Li Huang, Zhenfeng Zhang, Roger McMacken
doaj +1 more source
Mitochondrial DNA replication in sea urchin oocytes [PDF]
, 1974 Mitochondrial DNA (mtDNA) replicative intermediates from Strongylocentrotus purpuratus oocytes were isolated by ethidium bromide-CsCl density gradient centrifugation and examined by electron microscopy after formamide spreading.
Kasamatsu, Harumi +3 more
core +2 more sources
eLife, 2019 Transcription activator-like effectors (TALEs) bind DNA through an array of tandem 34-residue repeats. How TALE repeat domains wrap around DNA, often extending more than 1.5 helical turns, without using external energy is not well understood.
Kathryn Geiger-Schuller +3 more
doaj +1 more source
A helicoidal transfer matrix model for inhomogeneous DNA melting [PDF]
, 2006 An inhomogeneous helicoidal nearest-neighbor model with continuous degrees of freedom is shown to predict the same DNA melting properties as traditional long-range Ising models, for free DNA molecules in solution, as well as superhelically stressed DNA ...
D. Poland +3 more
core +2 more sources
Nature Communications, 2019 In the cell, DNA is arranged into highly-organised and topologically-constrained (supercoiled) structures. It remains unclear how this supercoiling affects the detailed double-helical structure of DNA, largely because of limitations in spatial resolution
Alice L. B. Pyne +12 more
semanticscholar +1 more source
Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations
eLife, 2020 Chromatin remodeling plays important roles in gene regulation during development, differentiation and in disease. The chromatin remodeling enzyme CHD4 is a component of the NuRD and ChAHP complexes that are involved in gene repression.
Lucas Farnung +2 more
doaj +1 more source
Molecular dynamics simulations of DNA-free and DNA-bound TAL effectors. [PDF]
PLoS ONE, 2013 TAL (transcriptional activator-like) effectors (TALEs) are DNA-binding proteins, containing a modular central domain that recognizes specific DNA sequences. Recently, the crystallographic studies of TALEs revealed the structure of DNA-recognition domain.
Hua Wan +4 more
doaj +1 more source
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation. [PDF]
PLoS Computational Biology, 2016 The ability to precisely modify genomes and regulate specific genes will greatly accelerate several medical and engineering applications. The CRISPR/Cas9 (Type II) system binds and cuts DNA using guide RNAs, though the variables that control its on ...
Iman Farasat, Howard M Salis
doaj +1 more source
Bubble statistics and positioning in superhelically stressed DNA
, 2011 We present a general framework to study the thermodynamic denaturation of double-stranded DNA under superhelical stress. We report calculations of position- and size-dependent opening probabilities for bubbles along the sequence. Our results are obtained
B. Alberts +4 more
core +1 more source
Mechanical response of plectonemic DNA: an analytical solution
, 2008 We consider an elastic rod model for twisted DNA in the plectonemic regime. The molecule is treated as an impenetrable tube with an effective, adjustable radius.
Allemand J. F. +31 more
core +2 more sources