Results 1 to 10 of about 1,985,752 (333)

Mammalian Expression Cloning of Nucleic Acid Binding Proteins by Agarose Thin-Layer Gelshift Clone Selection

BioTechniques, 2002
Here we report a functional screening technique to identify cDNAs encoding mammalian nucleic acid binding proteins. We have combined cDNA expression cloning with the agarose thin-layer gelshift assay technique to detect specific nucleic acid binding ...
A. Dobi, W. Debnam, C. Dalgard, A. Owusu, D.v. Agoston   +4 more
doaj   +1 more source

Human Cytomegalovirus UL34 Early and late Proteins Are Essential for Viral Replication

Viruses, 2014
UL34 is one of the ~50 genes of human cytomegalovirus (HCMV) required for replication in cell culture in human fibroblasts. UL34 encodes highly related early (UL34a) and late (UL34b) proteins that are virtually identical, with the early protein ...
Rico Rana, Bonita J. Biegalke
doaj   +1 more source

Mutagenic and Spectroscopic Investigation of pH Dependent CooA DNA Binding [PDF]

, 2017
The carbon monoxide (CO) sensing heme protein, CooA, is a transcription factor which exists in several bacteria that utilize CO as an energy source. CooA positively regulates the expression of coo genes in the presence of CO such that the corresponding ...
Weaver, Brian R.
core   +1 more source

Harnessing DNA Binding Proteins from Starved Cells for DNA Origami Protection

Small Structures
DNA origami is an attractive method for building structurally versatile and addressable nanostructures for diverse applications. The structural integrity of DNA, however, is rather limited in application‐relevant environments, such as in low‐magnesium ...
Sharon Saarinen, Alba Sanz‐Velasco, Paulina Indyka, Michał Rawski, Artur Biela, Eduardo Anaya‐Plaza, Mauri A. Kostiainen   +6 more
doaj   +1 more source

Identifying the “stripe” transcription factors and cooperative binding related to DNA methylation

Communications Biology
DNA methylation plays a critical role in gene regulation by modulating the DNA binding of transcription factors (TFs). This study integrates TFs’ ChIP-seq profiles with WGBS profiles to investigate how DNA methylation affects protein interactions ...
Ximei Luo, Quan Zou
doaj   +1 more source

The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study

BMC Immunology, 2002
Background The ZAS family is composed of proteins that regulate transcription via specific gene regulatory elements. The amino-DNA binding domain (ZAS-N) and the carboxyl-DNA binding domain (ZAS-C) of a representative family member, named κB DNA binding ...
Wu Lai-Chu, Mak Chi-ho, Allen Carl E
doaj   +1 more source

Phosphorylation of Adenovirus DNA-binding Protein

Journal of General Virology, 1989
Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with
W C, Russell, A, Webster, I R, Leith, G D, Kemp   +3 more
openaire   +2 more sources

The Influence of pH Variation on CooA Activity [PDF]

, 2016
CooA, a CO-sensing heme protein, acts as a transcriptional activator of CO-metabolizing proteins in bacteria such as Rhodospirillum rubrum and Carboxydothermus hydrogenoformans through sequence-specific DNA binding.
Clark, Robert W., DeVries, Rachael, Gunter, Amy, Weaver, Brian R   +3 more
core   +1 more source

Mechanism underlying synergic activation of Tyrosinase promoter by MITF and IRF4 [PDF]

, 2017
Background: The transcription factor interferon regulatory factor 4 (IRF4) was identified to be involved in human pigmentation by genome-wide association studies (GWASs).
Cai, Xinzhang, Chen, Hongsheng, Feng, Yong, He, Chufeng, Li, Jiada, Liu, Huadie, Liu, Xueming, Liu, Yalan, Mei, Lingyun, Peng, Zhen, Song, Jian, Vleminckx, Kris   +11 more
core   +1 more source

Structural basis for genome-wide site-specific DNA recognition by Nuclear Factor IA

Nature Communications
Nuclear Factor IA, a member of the long-studied Nuclear Factor I family of DNA-binding proteins, plays pivotal roles in development and metabolism. Dysregulation or loss of Nuclear Factor IA is associated with severe neurological defects in humans and ...
Ci Zhu, Ding Xiao, Zhipu Luo, Jie Zhang, Shuang Liu, Yueyu Wang, Xi Chen, Hejin Xiao, Xiaofeng Li, Juyu Tang, Xianyang Fang, Jie Shen, He Song   +12 more
doaj   +1 more source