Results 21 to 30 of about 5,403,999 (308)

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina, Bode, Bela Ernest; id_orcid, White, Malcolm F; id_orcid, Penedo, Carlos; id_orcid, Ackermann, Katrin; id_orcid   +17 more
core   +1 more source

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

, 2021
Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years,
Cubeddu, Liza (R16726), Gamsjaeger, Roland (R16936), Teegan Lawson, El-Kamand, Serene (S33745), Lawson, Teegan (S34513), Roland Gamsjaeger, Du Plessis, Mar-Dean (S34302), Mar-Dean Du Plessis, Liza Cubeddu, Serene El-Kamand   +9 more
core   +1 more source

Identification of DNA-binding proteins using support vector machines and evolutionary profiles

BMC Bioinformatics, 2007
Background Identification of DNA-binding proteins is one of the major challenges in the field of genome annotation, as these proteins play a crucial role in gene-regulation.
Gromiha Michael M, Kumar Manish, Raghava Gajendra PS   +2 more
doaj   +1 more source

Non-additivity in protein-DNA binding [PDF]

Bioinformatics, 2005
Localizing protein binding sites within genomic DNA is of considerable importance, but remains difficult for protein families, such as transcription factors, which have loosely defined target sequences. It is generally assumed that protein affinity for DNA involves additive contributions from successive nucleotide pairs within the target sequence. This
Ruadhan A. O'Flanagan, Guillaume Paillard, Richard Lavery, Anirvan M. Sengupta   +3 more
openaire   +3 more sources

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]

, 2012
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089), Antonia Jack (165084), Sandra B Hake, Patricia Wolf, Pichler Garwin, Pichler, Garwin, Hake, Sandra B., Antonia Jack, Wolf Patricia, Garwin Pichler (165082), Jack Antonia, Wolf, Patricia, Jack, Antonia, Hake Sandra B., Garwin Pichler, Patricia Wolf (165088)   +15 more
core   +2 more sources

Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)

Bio-Protocol, 2020
The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action.
Himasha Perera, Michael Trakselis
doaj   +1 more source

Single-stranded DNA binding proteins influence APOBEC3A substrate preference

Scientific Reports, 2021
The cytidine deaminase, APOBEC3A (A3A), is a prominent source of mutations in multiple cancer types. These APOBEC-signature mutations are non-uniformly distributed across cancer genomes, associating with single-stranded (ss) DNA formed during DNA ...
Amber L. Brown, Christopher D. Collins, Secily Thompson, Margo Coxon, Tony M. Mertz, Steven A. Roberts   +5 more
doaj   +1 more source

Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components [PDF]

, 2009
In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla.
Field, L.M., Pickett, J. A., Dufour, S. G. L., Zhou, J.J., Keep, N. H., Dufour, Samuel, Field, Linda M., He, X., Hooper, A.M., Hooper, A. M., Pickett, John A., Pickett, J.A., He, Xiaoli, Dufour, S., Field, L. M., Keep, Nicholas H., Robertson, G., Robertson, Giles, Zhou, J-J., Hooper, Antony M., Zhou, Jing-Jiang, Hooper, Tony, Pickett, John, Field, Lin   +23 more
core   +1 more source

Analyzing the Surface Structure of the Binding Domain on DNA and RNA Binding Proteins

IEEE Access, 2019
The study of nucleic acid-binding protein (NBP) has important significance for us to understand critical intracellular activities, such as the transmission of cellular genetic information, cell metabolism, substance transport, and signal transduction ...
Wei Wang, Keliang Li, Hehe Lv, Hongjun Zhang, Shiguang Zhang, Yun Zhou, Junwei Huang   +6 more
doaj   +1 more source

A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA

eLife, 2022
Clamp loaders place circular sliding clamp proteins onto DNA so that clamp-binding partner proteins can synthesize, scan, and repair the genome. DNA with nicks or small single-stranded gaps are common clamp-loading targets in DNA repair, yet these ...
Xingchen Liu, Christl Gaubitz, Joshua Pajak, Brian A Kelch   +3 more
doaj   +1 more source