Results 41 to 50 of about 5,403,999 (308)
Acrylamide Capture of DNA-Bound Complexes: Electrophoretic Purification of Transcription Factors
BioTechniques, 2002 We have developed a rapid nonradioactive electrophoretic technique to analyze proteins within DNA-binding complexes, acrylamide capture of DNA-binding complexes (ACDC), using Acrydite™-linked DNA-binding targets. The method is highly sensitive and easily
Colleen Nelson +3 more
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Comparison of histone-like HU protein DNA-binding properties and HU/IHF protein sequence alignment. [PDF]
PLoS ONE, 2017 The structure and function of bacterial nucleoid are controlled by histone-like proteins of HU/IHF family, omnipresent in bacteria and also founding archaea and some eukaryotes.HU protein binds dsDNA without sequence specificity and avidly binds DNA ...
Dmitri Kamashev +9 more
doaj +1 more source
Electrical Probes of DNA-Binding Proteins [PDF]
, 2017 A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins.
Barton, Jacqueline K. +3 more
openaire +4 more sources
DNA-binding activity of papillomavirus proteins [PDF]
Journal of Virology, 1987 We demonstrate DNA binding by papillomavirus (PV) open reading frame (ORF) proteins that correspond to the early transforming and trans-activating (E6 and E2) and late structural regions (L2 and L1) from bovine PV type 1 and human PV types 6b and 16.
R G, Mallon +2 more
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Calling cards for DNA-binding proteins [PDF]
Genome Research, 2007 Identifying genomic targets of transcription factors is fundamental for understanding transcriptional regulatory networks. Current technology enables identification of all targets of a single transcription factor, but there is no realistic way to achieve the converse: identification of all proteins that bind to a promoter of interest. We have developed
Wang, Haoyi +2 more
openaire +3 more sources
Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA [PDF]
, 2005 Baumgarth B, Bartels FW, Anselmetti D, Becker A, Ros R. Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA. Microbiology.
Baumgarth, Birgit +4 more
core +1 more source
Functional anatomy of polycomb and trithorax chromatin landscapes in Drosophila embryos. [PDF]
PLoS Biology, 2009 Polycomb group (PcG) and trithorax group (trxG) proteins are conserved chromatin factors that regulate key developmental genes throughout development.
Bernd Schuettengruber +8 more
doaj +1 more source
DNA G-Quadruplex-Binding Proteins: An Updated Overview
DNA, 2023 DNA G-quadruplexes (G4s) are non-canonical secondary structures formed in guanine-rich sequences. Within the human genome, G4s are found in regulatory regions such as gene promoters and telomeres to control replication, transcription, and telomere ...
Victoria Sanchez-Martin
doaj +1 more source
Protein–DNA binding in high-resolution [PDF]
Critical Reviews in Biochemistry and Molecular Biology, 2015 Recent advances in experimental and computational methodologies are enabling ultra-high resolution genome-wide profiles of protein-DNA binding events. For example, the ChIP-exo protocol precisely characterizes protein-DNA cross-linking patterns by combining chromatin immunoprecipitation (ChIP) with 5' → 3' exonuclease digestion.
Shaun, Mahony, B Franklin, Pugh
openaire +2 more sources
Chromosomal macrodomains and associated proteins : implications for DNA organization and replication in gram negative bacteria [PDF]
, 2011 The Escherichia coli chromosome is organized into four macrodomains, the function and organisation of which are poorly understood. In this review we focus on the MatP, SeqA, and SlmA proteins that have recently been identified as the first examples of ...
Kalmykowa, Olga J. +14 more
core +1 more source