Results 121 to 130 of about 665,876 (395)
BioMed Research International, 2016 The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) with 5′-3′ DNA polymerase activity while in absence of 5′-3′ exonuclease activity possesses high thermal stability and polymerase activity.
Yi Ma +5 more
semanticscholar +1 more source
Cell‐free and extracellular vesicle microRNAs with clinical utility for solid tumors
Molecular Oncology, EarlyView.Cell‐free microRNAs (cfmiRs) are small‐RNA circulating molecules detectable in almost all body biofluids. Innovative technologies have improved the application of cfmiRs to oncology, with a focus on clinical needs for different solid tumors, but with emphasis on diagnosis, prognosis, cancer recurrence, as well as treatment monitoring.
Yoshinori Hayashi +6 more
wiley +1 more source
A critical survey on the kinetic assays of DNA polymerase fidelity from a new theoretical perspective [PDF]
arXiv, 2020 The high fidelity of DNA polymerase is critical for the faithful replication of genomic DNA. Several approaches were proposed to quantify the fidelity of DNA polymerase. Direct measurements of the error frequency of the replication products definitely give the true fidelity but turn out very hard to implement.
arxiv
Label-free optical detection of single enzyme-reactant reactions and associated conformational changes [PDF]
, 2017 Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules. Detecting the enzyme-reactant interactions and associated conformational changes of the enzyme on a single molecule basis ...
arxiv +1 more source
Stochastics of DNA Quantification [PDF]
arXiv, 2023 A common approach to quantifying DNA involves repeated cycles of DNA amplification. This approach, employed by the polymerase chain reaction (PCR), produces outputs that are corrupted by amplification noise, making it challenging to accurately back-calculate the amount of input DNA.
arxiv
CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression
Genome Biology, 2023 The efficiency of homology-directed repair (HDR) plays a crucial role in the development of animal models and gene therapy. We demonstrate that microhomology-mediated end-joining (MMEJ) constitutes a substantial proportion of DNA repair during CRISPR ...
Hongyu Chen +14 more
doaj +1 more source
Cediranib suppresses homology-directed DNA repair through down-regulation of BRCA1/2 and RAD51
Science Translational Medicine, 2019 Cediranib directly suppresses homology-directed DNA repair in tumor cells and confers synthetic lethal vulnerability to PARP inhibitors. The secret life of cediranib Anti-angiogenic agents are used to inhibit the formation of new blood vessels that ...
Alanna R. Kaplan +6 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić +31 more
wiley +1 more source
DNA polymerase V activity is autoregulated by a novel intrinsic DNA-dependent ATPase [PDF]
arXiv, 2014 Escherichia coli DNA polymerase V (pol V), a heterotrimeric complex composed of UmuD'2C, is marginally active. ATP and RecA play essential roles in the activation of pol V for DNA synthesis including translesion synthesis (TLS). We have established three features of the roles of ATP and RecA.
arxiv
Genome Biology, 2017 Background The chromodomain helicase DNA-binding family of ATP-dependent chromatin remodeling factors play essential roles during eukaryote growth and development.
Rong Yang +13 more
doaj +1 more source