Results 31 to 40 of about 666,524 (370)
Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair. [PDF]
PLoS Genetics, 2009 Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results in impaired Vkappa-Jkappa recombination and altered somatic hypermutation and ...
Daniel Lucas +8 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1973 Two structurally distinct forms of RNA-directed DNA polymerase from avian myeloblastosis virus were resolved by chromatography on phosphocellulose and purified. In addition to RNA-directed DNA polymerase activity, both enzymes had ribonuclease H (RNase H)
D. Grandgenett, G. Gerard, M. Green
semanticscholar +1 more source
Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene [PDF]
Journal of Sciences, Islamic Republic of Iran, 2021 Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression.
Muhammad Azharuddin Azali +4 more
doaj +1 more source
Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis [PDF]
, 2018 Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues.
Baner +52 more
core +1 more source
Engineered DNA Polymerase Improves PCR Results for Plastid DNA
Applications in Plant Sciences, 2013 Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori +3 more
doaj +1 more source
New link between the RNA polymerase II-CTD and replication stress
Molecular & Cellular Oncology, 2021 Conflicts between transcription and replication are a major source of replication stress. Our recent findings show that proper dephosphorylation of Serine 5 in the carboxy-terminal domain (CTD) of DNA-directed RNA polymerase II subunit RPB1 is needed to ...
Helga B. Landsverk +3 more
doaj +1 more source
Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos.
Proceedings of the National Academy of Sciences of the United States of America, 1972 Early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous RNA-directed DNA polymerase activity. This endogenous DNA polymerase activity was not increased after mixture of soluble
C. Kang, H. Temin
semanticscholar +1 more source
Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
core +2 more sources
Proceedings of the National Academy of Sciences of the United States of America, 1972 Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes.
Bobrow Sn +3 more
semanticscholar +1 more source
RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis. [PDF]
, 2018 Small RNAs regulate chromatin modifications such as DNA methylation and gene silencing across eukaryotic genomes. In plants, RNA-directed DNA methylation (RdDM) requires 24-nucleotide small interfering RNAs (siRNAs) that bind to ARGONAUTE 4 (AGO4) and ...
Chory, Joanne +11 more
core +1 more source