Results 31 to 40 of about 235,447 (289)

Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians [PDF]

Immunity & Ageing, 2016
Recently, phage display technology has made it possible to define the circulating repertoire of humoral immunity. This study was designed to define the circulating antibodies specific to centenarians.We used a phage-displayed combinatorial peptide library to screen for peptides (YSATLRY and YSPTLFY) that preferentially react with the IgG fraction of ...
Han, Jungwon, Park, So-Hyun, Kim, Dong-Jo, Kim, Hyori, Choi, Yoon-Ho, Lee, Jong-Hyuk, Park, Sang Chul, Chung, Junho   +7 more
openaire   +4 more sources

Investigation of Multi-Subunit Mycobacterium tuberculosis DNA-Directed RNA Polymerase and Its Rifampicin Resistant Mutants

International Journal of Molecular Sciences, 2023
Emerging Mycobacterium tuberculosis (Mtb) resistant strains have continued to limit the efficacies of existing antitubercular therapies. More specifically, mutations in the RNA replicative machinery of Mtb, RNA polymerase (RNAP), have been widely linked to rifampicin (RIF) resistance, which has led to therapeutic failures in many clinical cases ...
Mokgerwa Zacharia Monama, Fisayo Olotu, Özlem Tastan Bishop   +2 more
openaire   +2 more sources

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme

Frontiers in Microbiology, 2016
Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme ...
Yi-Ping eWang, Wen-Juan eDu, Li-Ping eHuang, Yan-Wu eWei, Hong-Li eWu, Li eFeng, Chang-Ming eLiu   +6 more
doaj   +1 more source

Inhibitors of HIV-1 Reverse Transcriptase—Associated Ribonuclease H Activity

Biology, 2012
HIV-1 enzyme reverse transcriptase (RT) is a major target for antiviral drug development, with over half of current FDA-approved therapeutics against HIV infection targeting the DNA polymerase activity of this enzyme. HIV-1 RT is a multifunctional enzyme
Michael A. Parniak, Stefan G. Sarafianos, Rieko Ishima, Krystal LaBarge, Tatiana Ilina   +4 more
doaj   +1 more source

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

eLife, 2022
In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer, Jasleen Singh, Akihito Fukudome, Vibhor Mishra, Feng Wang, Craig S Pikaard   +5 more
doaj   +1 more source

Mode of inhibition of HIV reverse transcriptase by 2-hexaprenylhydroquinone, a novel general inhibitor of RNA-and DNA-directed DNA polymerases [PDF]

Biochemical Journal, 1997
A natural compound from the Red Sea sponge Ircinia sp., 2-hexaprenylhydroquinone (HPH), has been shown to be a general inhibitor of retroviral reverse transcriptases (from HIV-1, HIV-2 and murine leukaemia virus) as well as of cellular DNA polymerases (Escherichia coli DNA polymerase I, and DNA polymerases α and β).
Amnon Hizi, Shoshana Loya, Yoel Kashman, Amira Rudi   +3 more
openaire   +3 more sources

The Inhibitory Action of Kohamaic Acid A Derivatives on Mammalian DNA Polymerase β

Molecules, 2008
We previously isolated a novel natural product, designated kohamaic acid A (KA-A, compound 1), as an inhibitor of the first cleavage of fertilized sea urchin eggs, and found that this compound could selectively inhibit the activities of mammalian DNA ...
Yoshiyuki Mizushina, Daisuke Manita, Toshifumi Takeuchi, Yuko Kumamoto-Yonezawa, Yuki Matsui, Fumio Sugawara, Masaharu Takemura, Hirosato Takikawa, Hiromi Yoshida, Mitsuru Sasaki   +9 more
doaj   +1 more source

Engagement of Components of DNA-Break Repair Complex and NFκB in Hsp70A1A Transcription Upregulation by Heat Shock. [PDF]

PLoS ONE, 2017
An involvement of components of DNA-break repair (DBR) complex including DNA-dependent protein kinase (DNA-PK) and poly-ADP-ribose polymerase 1 (PARP-1) in transcription regulation in response to distinct cellular signalling has been revealed by ...
Joyita Hazra, Pooja Mukherjee, Asif Ali, Soumita Poddar, Mahadeb Pal   +4 more
doaj   +1 more source

Characterization of the Products of RNA-directed DNA Polymerases in Oncogenic RNA Viruses

Nature, 1970
Several RNA tumour viruses contain an enzyme that synthesizes a DNA–RNA hybrid using the single stranded viral RNA as template. Hybridization experiments confirm that the DNA strand is complementary to the viral RNA.
Spiegelman, S., Burny, Arsène, Das, M R, Keydar, J., Schlom, J., Travnicek, M., Watson, Kenneth M.   +6 more
openaire   +4 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source