Results 31 to 40 of about 665,876 (395)
PLoS ONE, 2013 Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3' terminal phosphodiester bond.
Alicia del Prado +4 more
doaj +1 more source
Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair. [PDF]
PLoS Genetics, 2009 Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results in impaired Vkappa-Jkappa recombination and altered somatic hypermutation and ...
Daniel Lucas +8 more
doaj +1 more source
Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene [PDF]
Journal of Sciences, Islamic Republic of Iran, 2021 Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression.
Muhammad Azharuddin Azali +4 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1973 Two structurally distinct forms of RNA-directed DNA polymerase from avian myeloblastosis virus were resolved by chromatography on phosphocellulose and purified. In addition to RNA-directed DNA polymerase activity, both enzymes had ribonuclease H (RNase H)
D. Grandgenett, G. Gerard, M. Green
semanticscholar +1 more source
Density- and elongation speed-dependent error correction in RNA polymerization [PDF]
, 2021 Backtracking of RNA polymerase (RNAP) is an important pausing mechanism during DNA transcription that is part of the error correction process that enhances transcription fidelity. We model the backtracking mechanism of RNA polymerase, which usually happens when the polymerase tries to incorporate a mismatched nucleotide triphosphate.
arxiv +1 more source
The mechanism of action of T7 DNA polymerase [PDF]
, 1998 The recent crystal structure determination of T7 DNA polymerase complexed to a deoxynucleoside triphosphate and primer-template DNA has provided the first glimpse of a replicative DNA polymerase in a catalytic complex.
Doublié, Sylvie, Ellenberger, Tom
core +1 more source
Engineered DNA Polymerase Improves PCR Results for Plastid DNA
Applications in Plant Sciences, 2013 Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori +3 more
doaj +1 more source
Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis [PDF]
, 2018 Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues.
Baner +52 more
core +1 more source
Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization [PDF]
, 2005 DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ...
Froeyen, Matheus +3 more
core +2 more sources
Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos.
Proceedings of the National Academy of Sciences of the United States of America, 1972 Early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous RNA-directed DNA polymerase activity. This endogenous DNA polymerase activity was not increased after mixture of soluble
C. Kang, H. Temin
semanticscholar +1 more source