Results 51 to 60 of about 666,524 (370)

T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis

Biotechnology & Biotechnological Equipment, 2016
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo, Shuhui Sun, Binghua Xie, Xiao-Jing Zhu, Zhong-Min Dai   +4 more
doaj   +1 more source

RNA-directed DNA polymerase and virus-induced leukemia in mice.

Proceedings of the National Academy of Sciences of the United States of America, 1973
Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme.
A. Wu, R. Ting, R. Gallo
semanticscholar   +1 more source

Interdependent interactions between TFIIB, TATA binding protein, and DNA. [PDF]

, 2002
Temperature-sensitive mutants of TFIIB that are defective for essential interactions were isolated. One mutation (G204D) results in disruption of a protein-protein contact between TFIIB and TATA binding protein (TBP), while the other (K272I) disrupts an ...
Buratowski, Robin M, Buratowski, Stephen, Downs, Jessica   +2 more
core   +2 more sources

Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians [PDF]

Immunity & Ageing, 2016
Recently, phage display technology has made it possible to define the circulating repertoire of humoral immunity. This study was designed to define the circulating antibodies specific to centenarians.We used a phage-displayed combinatorial peptide library to screen for peptides (YSATLRY and YSPTLFY) that preferentially react with the IgG fraction of ...
Han, Jungwon, Park, So-Hyun, Kim, Dong-Jo, Kim, Hyori, Choi, Yoon-Ho, Lee, Jong-Hyuk, Park, Sang Chul, Chung, Junho   +7 more
openaire   +4 more sources

Investigation of Multi-Subunit Mycobacterium tuberculosis DNA-Directed RNA Polymerase and Its Rifampicin Resistant Mutants

International Journal of Molecular Sciences, 2023
Emerging Mycobacterium tuberculosis (Mtb) resistant strains have continued to limit the efficacies of existing antitubercular therapies. More specifically, mutations in the RNA replicative machinery of Mtb, RNA polymerase (RNAP), have been widely linked to rifampicin (RIF) resistance, which has led to therapeutic failures in many clinical cases ...
Mokgerwa Zacharia Monama, Fisayo Olotu, Özlem Tastan Bishop   +2 more
openaire   +2 more sources

RNA polymerase V-dependent small RNAs in Arabidopsis originate from small, intergenic loci including most SINE repeats. [PDF]

, 2012
In plants, heterochromatin is maintained by a small RNA-based gene silencing mechanism known as RNA-directed DNA methylation (RdDM). RdDM requires the non-redundant functions of two plant-specific DNA-dependent RNA polymerases (RNAP), RNAP IV and RNAP V.
Chen, Xuemei, Gurazada, Sai Guna Ranjan, Lee, Tzuu-fen, Li, Shengben, Matzke, Marjori A, Meyers, Blake C, Simon, Stacey A, Zhai, Jixian   +7 more
core   +2 more sources

Inhibitors of HIV-1 Reverse Transcriptase—Associated Ribonuclease H Activity

Biology, 2012
HIV-1 enzyme reverse transcriptase (RT) is a major target for antiviral drug development, with over half of current FDA-approved therapeutics against HIV infection targeting the DNA polymerase activity of this enzyme. HIV-1 RT is a multifunctional enzyme
Michael A. Parniak, Stefan G. Sarafianos, Rieko Ishima, Krystal LaBarge, Tatiana Ilina   +4 more
doaj   +1 more source

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

eLife, 2022
In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer, Jasleen Singh, Akihito Fukudome, Vibhor Mishra, Feng Wang, Craig S Pikaard   +5 more
doaj   +1 more source

Role of RNA Interference (RNAi) in the Moss Physcomitrella patens [PDF]

, 2013
RNA interference (RNAi) is a mechanism that regulates genes by either transcriptional (TGS) or posttranscriptional gene silencing (PTGS), required for genome maintenance and proper development of an organism.
Arif, Muhammad Asif, Frank, Wolfgang, Khraiwesh, Basel   +2 more
core   +3 more sources

A model for transition of 5 '-nuclease domain of DNA polymerase I from inert to active modes [PDF]

, 2011
Bacteria contain DNA polymerase I (PolI), a single polypeptide chain consisting of similar to 930 residues, possessing DNA-dependent DNA polymerase, 3'-5' proofreading and 5'-3' exonuclease (also known as flap endonuclease) activities.
A Díaz, B Shen, C Orlando, CJ Bornarth, CM Joyce, CW Gardiner, DL Ho, E Delagoutte, H Risken, IV Kutyavin, Janine Santos, JJ Harrington, Jon R. Sayers, JR Sayers, JR Sayers, JR Sayers, K Datta, K Makiela-Dzbenska, KL Nakamaye, L Zheng, L Zietlow, LM Allen, M Imai, MR Lieber, MRG Hodskinson, P Robins, P Setlow, P Xie, P Xie, P Xie, PD Gutman, PG de Gennes, PH Patel, Ping Xie, PM Holland, PM Kampmeyer, RC Lundquist, RL Honeycutt, RM Turner, S Fukushima, SJ Garforth, TA Ceska, UK Urs, V Lyamichev, V Lyamichev, WS Kelley, Y Kim, Y Kurosawa, Y Nagata, Y Xu, Z Bu   +50 more
core   +8 more sources