Results 81 to 90 of about 665,876 (395)

Selection of chromosomal DNA libraries using a multiplex CRISPR system. [PDF]

, 2014
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that ...
Arkin, Adam P, Cate, Jamie HD, DeLoache, Will, Dueber, John E, Lee, Michael E, Li, Xin, Maurer, Matthew J, Poddar, Snigdha, Ryan, Owen W, Skerker, Jeffrey M, Tsai, Jordan C   +10 more
core   +2 more sources

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

The Rise and Fall of Billionaire siRNAs during Reproductive Development in Rice

Plants, 2022
RNA polymerase IV-dependent siRNAs, usually 24 nt in length, function in the RNA-directed DNA methylation that is responsible for de novo methylation in plants.
Lili Wang, Dachao Xu, Longjun Zeng, Dong-Lei Yang   +3 more
doaj   +1 more source

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]

, 2012
Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
core   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Mechanical Properties of Transcription [PDF]

Phys. Rev. Lett. 118, 268101 (2017), 2016
Recently the physical characterization of a number of biological processes has proven indispensable for a full understanding of natural phenomena. One such example is the mechanical properties of transcription, which have been shown to have significant effects in gene expression.
arxiv   +1 more source

A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments

BioTechniques, 2022
Despite the development of various methods and commercial kits, site-directed mutagenesis of large plasmids remains a challenge in many laboratories.
Zhibo Yang, Zan Chen, Yueping Zhang
doaj   +1 more source

An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation

Nature Plants, 2021
In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed ...
Meredith J. Sigman, Kaushik Panda, Rachel Kirchner, Lauren L. McLain, Hayden A Payne, John Reddy Peasari, Aman Y. Husbands, R. Slotkin, Andrea D. McCue   +8 more
semanticscholar   +1 more source

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis. [PDF]

, 2018
Small RNAs regulate chromatin modifications such as DNA methylation and gene silencing across eukaryotic genomes. In plants, RNA-directed DNA methylation (RdDM) requires 24-nucleotide small interfering RNAs (siRNAs) that bind to ARGONAUTE 4 (AGO4) and ...
Chory, Joanne, Duttke, Sascha H, Feng, Suhua, Gallego-Bartolome, Javier, Groth, Martin, Hetzel, Jonathan, Jacobsen, Steven E, Kuo, Hsuan Yu, Liu, Wanlu, Wang, Zonghua, Zhai, Jixian, Zhong, Zhenhui   +11 more
core   +1 more source