Results 31 to 40 of about 97,697 (191)

Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions

Biological Procedures Online, 2003
During a complex macromolecular reaction multiple changes in molecular conformation and interactions with ligands may occur. X-ray crystallography may provide only a limited set of snapshots of these changes.
Mukherjee Srabani, Sousa Rui
doaj   +1 more source

The RNA silencing enzyme RNA polymerase v is required for plant immunity. [PDF]

PLoS Genetics, 2011
RNA-directed DNA methylation (RdDM) is an epigenetic control mechanism driven by small interfering RNAs (siRNAs) that influence gene function. In plants, little is known of the involvement of the RdDM pathway in regulating traits related to immune ...
Ana López, Vicente Ramírez, Javier García-Andrade, Victor Flors, Pablo Vera   +4 more
doaj   +1 more source

NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.

Genes & Development, 2009
RNA-directed DNA methylation (RdDM) is an RNAi-based mechanism for establishing transcriptional gene silencing in plants. The plant-specific RNA polymerases IV and V are required for the generation of 24-nucleotide (nt) siRNAs and for guiding sequence ...
Xin‐Jian He, Yi-Feng Hsu, O. Pontes, Jianhua Zhu, Jian Lu, R. Bressan, C. Pikaard, Co-Shine Wang, Jian‐Kang Zhu   +8 more
semanticscholar   +1 more source

Isolation and physical properties of the DNA‐directed RNA polymerase from Thermus thermophilus HB8 [PDF]

European Journal of Biochemistry, 1990
The DNA‐directed RNA polymerase from the extremely thermophilic eubacterium Thermus thermophilus HB8 was purified employing a new and rapid method. The subunit pattern of the enzyme, analyzed by SDS gel electrophoresis, was interpreted as: 140 kDa and 170 kDa for β and β′, 40 kDa for α and 92 kDa for ó.
Stephan Wnendt, Roland K. Hartmann, Norbert Ulbrich, Volker A. Erdmann   +3 more
openaire   +3 more sources

Primer Extension Reactions for the PCR- based α- complementation Assay

Bio-Protocol, 2015
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the
Vasudevan Achuthan, Jeffrey DeStefano
doaj   +1 more source

Interplay among RNA polymerases II, IV and V in RNA-directed DNA methylation at a low copy transgene locus in Arabidopsis thaliana

Plant Molecular Biology, 2013
RNA-directed DNA methylation (RdDM) is an epigenetic process whereby small interfering RNAs (siRNAs) guide cytosine methylation of homologous DNA sequences. RdDM requires two specialized RNA polymerases: Pol IV transcribes the siRNA precursor whereas Pol
Wanhui You, Z. Lorković, A. Matzke, M. Matzke   +3 more
semanticscholar   +1 more source

Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians [PDF]

Immunity & Ageing, 2016
Recently, phage display technology has made it possible to define the circulating repertoire of humoral immunity. This study was designed to define the circulating antibodies specific to centenarians.We used a phage-displayed combinatorial peptide library to screen for peptides (YSATLRY and YSPTLFY) that preferentially react with the IgG fraction of ...
Han, Jungwon, Park, So-Hyun, Kim, Dong-Jo, Kim, Hyori, Choi, Yoon-Ho, Lee, Jong-Hyuk, Park, Sang Chul, Chung, Junho   +7 more
openaire   +4 more sources

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

Cell Reports, 2014
Summary: Unlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving ...
Jeremy R. Haag, Brent Brower-Toland, Elysia K. Krieger, Lyudmila Sidorenko, Carrie D. Nicora, Angela D. Norbeck, Andre Irsigler, Huachun LaRue, Jan Brzeski, Karen McGinnis, Sergey Ivashuta, Ljiljana Pasa-Tolic, Vicki L. Chandler, Craig S. Pikaard   +13 more
doaj   +1 more source

DNA-directed DNA Polymerase Activity in Oncogenic RNA Viruses

Nature, 1970
Besides having RNA-dependent DNA polymerase activity, oncogenic RNA viruses possess a DNA-directed DNA polymerase which is distinguished from previously described enzymes of this type in preferring double-stranded DNA as template and yielding a principally double-stranded product.
Spiegelman, S., Burny, Arsène, Das, M R, Keydar, J., Schlom, J., Travnicek, M., Watson, Kenneth M.   +6 more
openaire   +4 more sources

Inhibition of Ebola Virus by Anti-Ebola miRNAs in silico

Journal of Infection in Developing Countries, 2016
Introduction: MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate transcriptional and posttranscriptional gene regulation of the organisms. miRNA provides immune defense when the body is faced with challenges intracellular agents.
Zhabiz Golkar, Roshan Battaria, Donald G Pace, Omar Bagasra   +3 more
doaj   +1 more source