Results 41 to 50 of about 97,697 (191)

Structure and mechanism of the plant RNA polymerase V

open access: yesScience, 2023
In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants.
Guohui Xie   +6 more
semanticscholar   +1 more source

Promoter opening by sigma(54) and sigma(70) RNA polymerases: sigma factor-directed alterations in the mechanism and tightness of control.

open access: yesGenes & Development, 2000
Transcription control at the melting step is not yet understood. Here, band shift, cross-linking, and transcription experiments on diverse DNA probes were used with two bacterial RNA polymerase holoenzymes that differ in how they regulate melting.
Yuli Guo, C. M. Lew, J. Gralla
semanticscholar   +1 more source

Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure

open access: yesPLoS Biology, 2009
The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation.
Y. Korkhin   +7 more
semanticscholar   +1 more source

Atypical DNA methylation of genes encoding cysteine-rich peptides in Arabidopsis thaliana

open access: yesBMC Plant Biology, 2012
Background In plants, transposons and non-protein-coding repeats are epigenetically silenced by CG and non-CG methylation. This pattern of methylation is mediated in part by small RNAs and two specialized RNA polymerases, termed Pol IV and Pol V, in a ...
You Wanhui   +9 more
doaj   +1 more source

Isolation and characterization of monoclonal antibodies directed against two subunits of rabbit poxvirus-associated, DNA-directed RNA polymerase [PDF]

open access: yesJournal of Virology, 1985
A library of monoclonal antibodies directed against individual proteins of the rabbit poxvirus (RPV) virion within a complex immunogenic mixture has been generated through the use of in vivo and in vitro immunization regimens. The relative efficacies of the two procedures were compared.
D K Morrison, J K Carter, R W Moyer
openaire   +3 more sources

Structure and RNA template requirements of Arabidopsis RNA-DEPENDENT RNA POLYMERASE 2

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2021
Significance RDR2 is critical for siRNA-directed DNA methylation in Arabidopsis, functioning in physical association with DNA-dependent Pol IV to synthesize the second strands of double-stranded siRNA precursors.
Akihito Fukudome   +11 more
semanticscholar   +1 more source

Distinct transcriptional responses of RNA polymerases I, II and III to aptamers that bind TBP

open access: yesNucleic Acids Research, 2005
The TATA-binding protein (TBP) is a general factor that is involved in transcription by all three types of nuclear RNA polymerase. To delineate the roles played by the DNA-binding surface of TBP in these transcription reactions, we used a set of RNA ...
Xiaochun Fan, Hua Shi, J. Lis
semanticscholar   +1 more source

Expression, crystallization and preliminary X-ray crystallographic analysis of DNA-directed RNA polymerase subunit L fromThermococcus onnurineusNA1 [PDF]

open access: yesActa Crystallographica Section F Structural Biology Communications, 2014
RNA polymerase (RNAP) plays a crucial role in gene expression in all organisms. It is a multiprotein complex that produces primary transcript RNA. Generally, the basal transcription apparatus in archaea is simpler than the eukaryotic RNA polymerase II counterpart.
Lin Woo Kang   +3 more
openaire   +3 more sources

Purification and partial characterization of an entomopoxvirus (DlEPV) from a parasitic wasp of tephritid fruit flies

open access: yesJournal of Insect Science, 2002
An insect poxvirus [entomopoxvirus (EPV)] occurs in the poison gland apparatus of female Diachasmimorpha longicaudata , a parasitic wasp of the Caribbean fruit fly, Anastrepha suspensa and other tephritid fruit flies.
Pauline O. Lawrence
doaj  

Genetic and structural study of DNA-directed RNA polymerase II of Trypanosoma brucei, towards the designing of novel antiparasitic agents

open access: yesPeerJ, 2017
Trypanosoma brucei brucei (TBB) belongs to the unicellular parasitic protozoa organisms, specifically to the Trypanosoma genus of the Trypanosomatidae class. A variety of different vertebrate species can be infected by TBB including humans and animals.
Vasileios Megalooikonomou   +5 more
openaire   +4 more sources

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