Results 11 to 20 of about 5,185,708 (294)

Linear DNA amplicons as a novel cancer vaccine strategy

Journal of Experimental & Clinical Cancer Research, 2022
Background DNA-based vaccines represent a simple, safe and promising strategy for harnessing the immune system to fight infectious diseases as well as various forms of cancer and thus are considered an important tool in the cancer immunotherapy toolbox ...
Antonella Conforti, Erika Salvatori, Lucia Lione, Mirco Compagnone, Eleonora Pinto, Clay Shorrock, James A. Hayward, Yuhua Sun, Ben Minghwa Liang, Fabio Palombo, Brian Viscount, Luigi Aurisicchio   +11 more
doaj   +1 more source

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Nature, 2016
Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target locus as the first step to gene correction. Although most genetic diseases arise from point mutations, current approaches to point mutation correction are ...
Alexis C. Komor, Yongjoo B. Kim, M. Packer, John A. Zuris, David R. Liu   +4 more
semanticscholar   +1 more source

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Genome Biology, 2009
Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of ...
Ben Langmead, C. Trapnell, Mihai Pop, S. Salzberg   +3 more
semanticscholar   +1 more source

Transcriptome analysis of Clavibacter michiganensis subsp. michiganensis-infected tomatoes: a role of salicylic acid in the host response

BMC Plant Biology, 2021
Bacterial canker of tomato (Solanum lycopersicon) caused by the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is an economically important disease.
Naoki Yokotani, Yoshinori Hasegawa, Masaru Sato, Hideki Hirakawa, Yusuke Kouzai, Yoko Nishizawa, Eiji Yamamoto, Yoshiki Naito, Sachiko Isobe   +8 more
doaj   +1 more source

DNA sequencing with chain-terminating inhibitors.

Proceedings of the National Academy of Sciences of the United States of America, 1977
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol.
F. Sanger, S. Nicklen, A. Coulson
semanticscholar   +1 more source

Identification of QTLs for root color and carotenoid contents in Japanese orange carrot F2 populations

Scientific Reports, 2022
Carrot is a major source of provitamin A in a human diet. Two of the most important traits for carrot breeding are carotenoid contents and root color.
Taeko Shibaya, Chika Kuroda, Hisano Tsuruoka, Chiharu Minami, Akiko Obara, Shinobu Nakayama, Yoshie Kishida, Takayoshi Fujii, Sachiko Isobe   +8 more
doaj   +1 more source

An Integrated Encyclopedia of DNA Elements in the Human Genome

Nature, 2012
The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of transcription, transcription factor
ENCODEConsortium, Martin Renqiang Min
semanticscholar   +1 more source

CRISPAltRations: A validated cloud-based approach for interrogation of double-strand break repair mediated by CRISPR genome editing

Molecular Therapy: Methods & Clinical Development, 2021
CRISPR systems enable targeted genome editing in a wide variety of organisms by introducing single- or double-strand DNA breaks, which are repaired using endogenous molecular pathways.
Gavin Kurgan, Rolf Turk, Heng Li, Nathan Roberts, Garrett R. Rettig, Ashley M. Jacobi, Lauren Tso, Morgan Sturgeon, Massimo Mertens, Roel Noten, Kurt Florus, Mark A. Behlke, Yu Wang, Matthew S. McNeill   +13 more
doaj   +1 more source

Activation of the IRE1 RNase through remodeling of the kinase front pocket by ATP-competitive ligands

Nature Communications, 2020
The RNase activity of Inositol-Requiring Enzyme 1 (IRE1) can be allosterically regulated by ATP-competitive inhibitors of the IRE1 kinase domain. Here, the authors identify ATP-competitive IRE1 RNase activators with improved selectivity and cellular ...
Elena Ferri, Adrien Le Thomas, Heidi Ackerly Wallweber, Eric S. Day, Benjamin T. Walters, Susan E. Kaufman, Marie-Gabrielle Braun, Kevin R. Clark, Maureen H. Beresini, Kyle Mortara, Yung-Chia A. Chen, Breanna Canter, Wilson Phung, Peter S. Liu, Alfred Lammens, Avi Ashkenazi, Joachim Rudolph, Weiru Wang   +17 more
doaj   +1 more source

Author Correction: Artificial neural network, predictor variables and sensitivity threshold for DNA methylation-based age prediction using blood samples

Scientific Reports, 2021
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Zhonghui Thong, Jolena Ying Ying Tan, Eileen Shuzhen Loo, Yu Wei Phua, Xavier Liang Shun Chan, Christopher Kiu-Choong Syn   +5 more
doaj   +1 more source