Results 11 to 20 of about 6,548,876 (337)
DNA methylation and DNA methyltransferases [PDF]
Epigenetics & Chromatin, 2017 The prevailing views as to the form, function, and regulation of genomic methylation patterns have their origin many years in the past, at a time when the structure of the mammalian genome was only dimly perceived, when the number of protein-encoding ...
Bestor, Timothy H +3 more
core +10 more sources
mAbs, 2022 Rapid release of biopharmaceutical products enables a more efficient drug manufacturing process. Multi-attribute methods that target several product quality attributes (PQAs) at one time are an essential pillar of the rapid-release strategy.
Bingchuan Wei +25 more
doaj +1 more source
Proceedings of the National Academy of Sciences, 1998 A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This “deoxyribozyme” can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates.
Shameelah R. Balkhi +2 more
openaire +3 more sources
Microbiome, 2023 Background IL-22 is induced by aryl hydrocarbon receptor (AhR) signaling and plays a critical role in gastrointestinal barrier function through effects on antimicrobial protein production, mucus secretion, and epithelial cell differentiation and ...
Jordan S. Mar +11 more
doaj +1 more source
Phosphorylating DNA with DNA [PDF]
Proceedings of the National Academy of Sciences, 1999 Nearly 50 individual DNAs with polynucleotide kinase-like activity were isolated from a random-sequence pool by using in vitro selection. Each self-phosphorylating deoxyribozyme makes use of one or more of the eight standard NTPs or dNTPs as a source of activated phosphate.
Yingfu Li, Ronald R. Breaker
openaire +3 more sources
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
Scientific Reports, 2021 CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR ...
Mollie S. Schubert +7 more
doaj +1 more source
Cohesin causes replicative DNA damage by trapping DNA topological stress [PDF]
, 2020 DNA topological stress inhibits DNA replication fork (RF) progression and contributes to DNA replication stress. In Saccharomyces cerevisiae, we demonstrate that centromeric DNA and the rDNA array are especially vulnerable to DNA topological stress ...
Baxter, Jonathan +2 more
core +1 more source
BMC Cancer, 2023 Background KRAS mutations occur frequently in advanced non-small cell lung cancer (aNSCLC); the G12C mutation is the most prevalent. Alterations in STK11 or KEAP1 commonly co-occur with KRAS mutations in aNSCLC.
Cristina Julian +7 more
doaj +1 more source
Biophysical Journal, 2017 AbstractThe three-dimensional structure of DNA is highly susceptible to changes by mechanical and biochemical cues in vivo and in vitro. In particular, large increases in base pair spacing compared to regular B-DNA are effected by mechanical (over)stretching and by intercalation of compounds that are widely used in biophysical/chemical assays and drug ...
Koen Schakenraad +8 more
openaire +7 more sources
BMC Plant Biology, 2023 Background Plant genome information is fundamental to plant research and development. Along with the increase in the number of published plant genomes, there is a need for an efficient system to retrieve various kinds of genome-related information from ...
Hisako Ichihara +17 more
doaj +1 more source