Results 41 to 50 of about 4,554,738 (310)

Central Nervous System Tumors Among Infants in Canada: A Report From CYP‐C

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Central nervous system (CNS) tumors in infants are rare, pose unique clinical challenges, and lack large‐scale evidence‐based data to guide management. This study seeks to describe CNS tumors in Canadian infants and to compare their outcomes with those of older children.
Samuel Sassine, Hallie Coltin, Sylvia Cheng, Lucie Lafay‐Cousin, Vanan Issai Magimairajan, Craig Erker, Stéphanie Vairy, Lynette Bowes, Adam Fleming, Donna Johnston, Sarah McKillop, Roona Sinha, Julie Bennett, Christina Coleman, Laura Wheaton, Shayna Zelcer, Samuele Renzi, Thai Hoa Tran   +17 more
wiley   +1 more source

Use of High Specific Activity StarFire™ Oligonucleotide Probes to Visualize Low-Abundance Pre-mRNA Splicing Intermediates in S. pombe

BioTechniques, 2000
An oligonucleotide labeling system was developed that can produce radiolabeled hybridization probes with tenfold or more higher specific activity than is obtained by traditional 5¢-end-labeling with polynucleotide kinase.
M.A. Behlke, S.A. Dames, W.H. McDonald, K.L. Gould, E.J. Devor, J.A. Walder   +5 more
doaj   +1 more source

AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

Nature Communications, 2021
The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.
Liyang Zhang, John A. Zuris, Ramya Viswanathan, Jasmine N. Edelstein, Rolf Turk, Bernice Thommandru, H. Tomas Rube, Steve E. Glenn, Michael A. Collingwood, Nicole M. Bode, Sarah F. Beaudoin, Swarali Lele, Sean N. Scott, Kevin M. Wasko, Steven Sexton, Christopher M. Borges, Mollie S. Schubert, Gavin L. Kurgan, Matthew S. McNeill, Cecilia A. Fernandez, Vic E. Myer, Richard A. Morgan, Mark A. Behlke, Christopher A. Vakulskas   +23 more
doaj   +1 more source

DMSO Improves the Ski-Slope Effect in Direct PCR

Applied Sciences, 2021
Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA ...
Joo-Young Kim, Ju Yeon Jung, Da-Hye Kim, Seohyun Moon, Won-Hae Lee, Byung-Won Chun, Dong-Ho Choi   +6 more
doaj   +1 more source

Comparative Drug Response Profiling in Neuroblastoma Cell Lines and Patient‐Derived Tumor Organoids

Pediatric Blood &Cancer, EarlyView.
ABSTRACT High‐risk neuroblastoma remains a leading cause of pediatric cancer mortality, and improved preclinical models are needed to guide therapeutic developments. We screened seven high‐risk neuroblastoma cell lines and three patient‐derived tumor organoids with 528 compounds alongside bone marrow controls, and compared them with external datasets ...
Krzysztof Wierbiłowicz, Se Whee Sammy Park, Aironas Los, Ernest Liu, Nona Struyf, Tom Erkers, Sören Lehmann, Jan J. Molenaar, Jakob Stenman, Olli Kallioniemi, Päivi Östling, Brinton Seashore‐Ludlow, Kasper Karlsson   +12 more
wiley   +1 more source

Maternal nutritional status, C1 metabolism and offspring DNA methylation: a review of current evidence in human subjects. [PDF]

, 2011
: Evidence is growing for the long-term effects of environmental factors during early-life on later disease susceptibility. It is believed that epigenetic mechanisms (changes in gene function not mediated by DNA sequence alteration), particularly DNA ...
Dominguez Salas, Paula, Dominguez-Salas, P, Prentice, AM, Sophie E. Moore, Cox, SE, Dominguez-Salas, Paula, Prentice, Andrew M, Hennig, Branwen J, Paula Dominguez-Salas, Andrew M. Prentice, Moore, SE, Moore, Sophie E, Hennig, BJ, Cox, Sharon E, Prentice, Andrew M., Moore, Sophie E., Hennig, Branwen J., Branwen J. Hennig, Sharon E. Cox, Cox, Sharon E.   +19 more
core   +1 more source

Impact of Radiation Therapy on Physical and Psychosocial Health of Adolescents and Young Adults: A Joint Report From the Children's Oncology Group AYA and Radiation Oncology Committees

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Rates of cancer among adolescents and young adults (AYA), age 15–39 years, are increasing. Consequently, radiation oncologists are treating more AYAs who have diagnoses spanning both pediatric and adult practices. Compared to pediatric and older adult patients, AYAs face a unique set of challenges.
Hesham Elhalawani, Rachel K. Peterson, Avani D. Rao, Laurie J. McKenzie, Ahmed Magdy Elmotayam, Leslie Chang, Michael Roth, Susan L. McGovern   +7 more
wiley   +1 more source

A DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B and T cell responses in outbred animals [PDF]

, 2007
We developed a vaccine construct in which a BVP22 domain and an invariant-chain major histocompatibility complex class II-targeting motif capable of enhancing dendritic cell antigen uptake and presentation were fused to a sequence encoding a B- and T ...
Palmer, Guy H, Zhuang, Yan, Mwangi, W, Howard, CJ, Aida, Yoko, Hunter, Beverly J, Splitter, Gary A, Brown, WC, Palmer, GH, Hunter, BJ, Aida, Y, Davies, Christopher J, Brown, Wendy C, Davies, CJ, Mwangi, Waithaka, Splitter, GA, Zhuang, Y, Hope, Jayne; id_orcid, Howard, Chris J, Hope, Jayne C   +19 more
core   +1 more source

UNCOVERseq enables sensitive and controlled gene editing off-target nomination across CRISPR-Cas modalities and systems

Nature Communications
The rapid expansion of CRISPR-Cas gene editing enables new therapeutic strategies but complicates assessment of unintended editing risks due to emerging modalities and unclear analytical standards. We present UNCOVERseq (Unbiased Nomination of CRISPR Off-
Kyle J. Kinney, Kun Jia, He Zhang, Ellen Schmaljohn, Thomas Osborne, Bernice Thommandru, Karthik Murugan, Andrea Sánchez-Peña, Sean West, Shengyao Chen, Roshani Codipilly, Morgan Sturgeon, Rolf Turk, Matthew S. McNeill, Mark Behlke, Ashley Jacobi, M. Kyle Cromer, Garrett Rettig, Gavin L. Kurgan   +18 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source