Results 21 to 30 of about 902,693 (268)

DNA damage and autophagy [PDF]

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2011
Both exogenous and endogenous agents are a threat to DNA integrity. Exogenous environmental agents such as ultraviolet (UV) and ionizing radiation, genotoxic chemicals and endogenous byproducts of metabolism including reactive oxygen species can cause alterations in DNA structure (DNA damage). Unrepaired DNA damage has been linked to a variety of human
Humberto, Rodriguez-Rocha, Aracely, Garcia-Garcia, Mihalis I, Panayiotidis, Rodrigo, Franco   +3 more
openaire   +2 more sources

Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

BioTechniques, 2014
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC ...
Maxime François, Kevin Hochstenbach, Wayne Leifert, Michael Felix Fenech   +3 more
doaj   +1 more source

8-Oxo-7,8-Dihydro-2′-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2′-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development

Molecules, 2020
The growing clinical and epidemiological significance of gestational diabetes mellitus results from its constantly increasing worldwide prevalence, obesity, and overall unhealthy lifestyle among women of childbearing age. Oxidative stress seems to be the
Sandra K. Urbaniak, Karolina Boguszewska, Michał Szewczuk, Julia Kaźmierczak-Barańska, Bolesław T. Karwowski   +4 more
doaj   +1 more source

The Usefulness of Autoradiography for DNA Repair Proteins Activity Detection in the Cytoplasm towards Radiolabeled Oligonucleotides Containing 5′,8-Cyclo-2′-deoxyadenosine

Chemosensors, 2022
Autoradiography of 32P-radiolabeled oligonucleotides is one of the most precise detection methods of DNA repair processes. In this study, autoradiography allowed assessing the activity of proteins in the cytoplasm involved in DNA repair. The cytoplasm is
Karolina Boguszewska, Bolesław T. Karwowski, Julia Kaźmierczak-Barańska   +2 more
doaj   +1 more source

FANCD2 and DNA Damage [PDF]

International Journal of Molecular Sciences, 2017
Investigators have dedicated considerable effort to understanding the molecular basis underlying Fanconi Anemia (FA), a rare human genetic disease featuring an extremely high incidence of cancer and many congenital defects. Among those studies, FA group D2 protein (FANCD2) has emerged as the focal point of FA signaling and plays crucial roles in ...
Manoj Nepal, Raymond Che, Chi Ma, Jun Zhang, Peiwen Fei   +4 more
openaire   +2 more sources

Novel Implications of DNA Damage Response in Drug Resistance of Malignant Cancers Obtained from the Functional Interaction between p53 Family and RUNX2

Biomolecules, 2015
During the lifespan of cells, their genomic DNA is continuously exposed to theendogenous and exogenous DNA insults. Thus, the appropriate cellular response to DNAdamage plays a pivotal role in maintaining genomic integrity and also acts as a ...
Toshinori Ozaki, Mizuyo Nakamura, Osamu Shimozato   +2 more
doaj   +1 more source

Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase

mBio, 2023
DNA-damage tolerance (DDT) is a mechanism by which eukaryotes bypass replication-blocking lesions to resume DNA synthesis and maintain cell viability.
Li Fan, Wenqing Zhang, Josephine Rybchuk, Yu Luo, Wei Xiao   +4 more
doaj   +1 more source

PTPRK suppresses progression and chemo‐resistance of colon cancer cells via direct inhibition of pro‐oncogenic CD133

FEBS Open Bio, 2019
Receptor‐type protein tyrosine phosphatase κ (PTPRK) is considered to be a candidate tumor suppressor. PTPRK dephosphorylates CD133, which is a stem cell marker; phosphorylated CD133 accelerates xenograft tumor growth of colon cancer cells through the ...
Masashi Matsushita, Yusuke Mori, Kyosuke Uchiumi, Takehiro Ogata, Mizuyo Nakamura, Hiroyuki Yoda, Hiroaki Soda, Nobuhiro Takiguchi, Yoshihiro Nabeya, Osamu Shimozato, Toshinori Ozaki   +10 more
doaj   +1 more source

The Similarities between Human Mitochondria and Bacteria in the Context of Structure, Genome, and Base Excision Repair System

Molecules, 2020
Mitochondria emerged from bacterial ancestors during endosymbiosis and are crucial for cellular processes such as energy production and homeostasis, stress responses, cell survival, and more.
Karolina Boguszewska, Michał Szewczuk, Julia Kaźmierczak-Barańska, Bolesław T. Karwowski   +3 more
doaj   +1 more source

DNA damage and repair [PDF]

Nature, 2003
The aesthetic appeal of the DNA double helix initially hindered notions of DNA mutation and repair, which would necessarily interfere with its pristine state. But it has since been recognized that DNA is subject to continuous damage and the cell has an arsenal of ways of responding to such injury.
openaire   +2 more sources