Results 21 to 30 of about 1,590,914 (306)

DNA Checkpoint and Repair Factors Are Nuclear Sensors for Intracellular Organelle Stresses-Inflammations and Cancers Can Have High Genomic Risks. [PDF]

, 2018
Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability.
Chen, Hongping, Choi, Eric T., Cueto, Ramon, Drosatos, Konstantinos, Fu, Hangfei, Nanayakkara, Gayani K, Park, Joon Y., Qin, Xuebin, Shao, Lijian, Shao, Ying, Sheng, Haitao, Sun, Jianxin, Sun, Yu, Wang, Hong, Wang, Luqiao, Wu, Na, Yang, Qian, Yang, William Y, Yang, Wuping, Yang, Xiaofeng, Zeng, Huihong, Zhu, Qingxian   +21 more
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Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

BioTechniques, 2014
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC ...
Maxime François, Kevin Hochstenbach, Wayne Leifert, Michael Felix Fenech   +3 more
doaj   +1 more source

8-Oxo-7,8-Dihydro-2′-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2′-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development

Molecules, 2020
The growing clinical and epidemiological significance of gestational diabetes mellitus results from its constantly increasing worldwide prevalence, obesity, and overall unhealthy lifestyle among women of childbearing age. Oxidative stress seems to be the
Sandra K. Urbaniak, Karolina Boguszewska, Michał Szewczuk, Julia Kaźmierczak-Barańska, Bolesław T. Karwowski   +4 more
doaj   +1 more source

The Usefulness of Autoradiography for DNA Repair Proteins Activity Detection in the Cytoplasm towards Radiolabeled Oligonucleotides Containing 5′,8-Cyclo-2′-deoxyadenosine

Chemosensors, 2022
Autoradiography of 32P-radiolabeled oligonucleotides is one of the most precise detection methods of DNA repair processes. In this study, autoradiography allowed assessing the activity of proteins in the cytoplasm involved in DNA repair. The cytoplasm is
Karolina Boguszewska, Bolesław T. Karwowski, Julia Kaźmierczak-Barańska   +2 more
doaj   +1 more source

Nuclear rupture at sites of high curvature compromises retention of DNA repair factors. [PDF]

, 2018
The nucleus is physically linked to the cytoskeleton, adhesions, and extracellular matrix-all of which sustain forces, but their relationships to DNA damage are obscure. We show that nuclear rupture with cytoplasmic mislocalization of multiple DNA repair
Alshareeda, Alvey, Andrea J. Liu, Broers, Burma, Buxboim, Buxboim, Capo-chichi, Chang, Charlotte R. Pfeifer, Coffinier, Cory M. Alvey, Cumming, Darzynkiewicz, Das, Dazhen Liu, de la Rosa, De Vos, Denais, Dennis E. Discher, Dingal, Docheva, Elosegui-Artola, Fong, Frigault, Grundy, Gudmundsdottir, Harada, Harding, Hatch, Hategan, Hernandez, Irena L. Ivanovska, Irianto, Ivanovska, Jerome Irianto, Jiazheng Ji, Kaspi, Kuangzheng Zhu, Lammerding, Larrieu, Levental, Lucas Smith, Nuciforo, Odde, Oh, Raab, Rachel R. Bennett, Roger A. Greenberg, Saez, Samudram, Sangkyun Cho, Schneider, Seluanov, Shin, Soubeyrand, Staszewski, Swift, Takaki, Tamiello, Tang, Turgay, Wang, Yuntao Xia, Álvarez-Quilón   +64 more
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RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]

, 2020
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe, García Domínguez, Mario, Herrero Ruiz, Andrés M., Moreno Oñate, Marta, Ruiz Pérez, José Francisco   +4 more
core   +1 more source

The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins [PDF]

, 2019
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human DNA damage response (DDR) genes ...
Aguilera López, Andrés, Barroso Ceballos, Sonia Inés, García Rubio, María Luisa, Gómez González, Belén, Herrera Moyano, Emilia, Muñoz Sánchez, Sergio   +5 more
core   +1 more source

Cx43 channels and signaling via IP3/Ca2+, ATP, and ROS/NO propagate radiation-induced DNA damage to non-irradiated brain microvascular endothelial cells [PDF]

, 2020
Radiotherapeutic treatment consists of targeted application of radiation beams to a tumor but exposure of surrounding healthy tissue is inevitable.
Bultynck, Geert, De Bock, Marijke, De Smet, Maarten, Decrock, Elke, Delvaeye, Tinneke, Descamps, Benedicte, Hoorelbeke, Delphine, Krysko, Dmitri, Leybaert, Luc, Van Haver, Valérie, Vanhove, Christian   +10 more
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Novel Implications of DNA Damage Response in Drug Resistance of Malignant Cancers Obtained from the Functional Interaction between p53 Family and RUNX2

Biomolecules, 2015
During the lifespan of cells, their genomic DNA is continuously exposed to theendogenous and exogenous DNA insults. Thus, the appropriate cellular response to DNAdamage plays a pivotal role in maintaining genomic integrity and also acts as a ...
Toshinori Ozaki, Mizuyo Nakamura, Osamu Shimozato   +2 more
doaj   +1 more source

Inflammation-induced DNA damage and damage-induced inflammation: a vicious cycle [PDF]

, 2014
Inflammation is the ultimate response to the constant challenges of the immune system by microbes, irritants or injury. The inflammatory cascade initiates with the recognition of microorganism-derived pathogen associated molecular patterns (PAMPs) and ...
Abe, Alexeyev, Andersson, Andersson, Ba, Babior, Bartoloni, Berger, Blyth, Brill, Brinkmann, Cadet, Collins, Cooke, Csanád Z. Bachrati, David, Ding, Dostert, Ermakov, Ermakov, Ferguson, Fuchs, Gahan, Gedik, Gehrke, Hajizadeh, Halle, Hiramoto, Huang, Huang, Huang, Huttunen, Ishikawa, Ivana, Ivanov, Jahr, Jia, Kang, Kanneganti, Kazak, Klebanoff, Knaapen, Kondo, Kostyuk, Kostyuk, Krieg, Kuechle, Lane, Lange, Latz, Li, Malins, Mariathasan, Munoz-Gamez, Nakahira, Oka, Pacher, Parvatiyar, Pasheva, Pinti, Polk, Rathinam, Saitoh, Saxena, Scaffidi, Schroder, Shimada, Shokolenko, Sun, Suter, Suzuki, Swindall, Tadie, Taguchi, Takeda, Takeda, Tang, Tang, Thomson, Timea Pálmai-Pallag, Unterholzner, Urbonaviciute, Venereau, Volcic, Weinberg, Weiss, Wilson, Wu, Yan, Yang, Yoshida, Yoshihara, Yotsumoto, You, Yousefi, Zhang, Zhang, Zhang, Zhang, Zhang, Zhou   +100 more
core   +1 more source