Results 41 to 50 of about 888,680 (335)
Editing and escape from editing in anti-DNA B cells [PDF]
Proceedings of the National Academy of Sciences, 2008 Tolerance to dsDNA is achieved through editing of Ig receptors that react with dsDNA. Nevertheless, some B cells with anti-dsDNA receptors escape editing and migrate to the spleen. Certain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transgenic mice also bind an additional, Golgi-associated antigen. B cells
Salar N, Khan +10 more
openaire +2 more sources
DNA Damage Response Pathway and Replication Fork Stress During Oligonucleotide Directed Gene Editing
Molecular Therapy: Nucleic Acids, 2012 Single-stranded DNA oligonucleotides (ODNs) can be used to direct the exchange of nucleotides in the genome of mammalian cells in a process known as gene editing.
Melissa Bonner +4 more
doaj +1 more source
Communications Biology, 2023 Adenosine-to-inosine tRNA-editing enzyme has been identified for more than two decades, but the study on its DNA editing activity is rather scarce. We show that amphioxus (Branchiostoma japonicum) ADAT2 (BjADAT2) contains the active site ‘HxE-PCxxC’ and ...
Zhan Gao +10 more
doaj +1 more source
Explore the dominant factor in prime editing via a view of DNA processing
Synthetic and Systems Biotechnology, 2023 Prime editing is a revolutionary gene-editing method that is capable of introducing insertions, deletions and base substitutions into the genome. However, the editing efficiency of Prime Editor (PE) is limited by the DNA repair process.
Zhimeng Xu +6 more
doaj +1 more source
Advances in biotechnology: genomics and genome editing [PDF]
, 2017 Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso +4 more
core +1 more source
Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]
, 2018 Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson +22 more
core +1 more source
DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements
BMC Biology, 2023 Background Recent studies have shown that, owning to its cohesive cleavage, Cas9-mediated CRISPR gene editing outcomes at junctions of chromosomal rearrangements or DNA-fragment editing are precise and predictable; however, the underlying mechanisms are ...
Mohammadreza M. Mehryar +3 more
doaj +1 more source
In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing. [PDF]
mBio, 2015 The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, an RNA-guided nuclease for specific genome editing in vivo, has been adopted in a wide variety of organisms.
Liu Y +6 more
europepmc +2 more sources
CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
core +1 more source
Molecular Therapy: Nucleic Acids, 2022 Strategies to modulate cellular DNA repair pathways hold immense potential to enhance the efficiency of CRISPR-Cas9 genome editing platform. In the absence of a repair template, CRISPR-Cas9-induced DNA double-strand breaks are repaired by the endogenous ...
Tarun Mishra +6 more
doaj +1 more source