CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]
, 2020 With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu +6 more
core
Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]
, 2020 Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu +2 more
core +2 more sources
Universal RNA editing in a human liver at the fetal stage [PDF]
, 2012 It is known that RNA editing occurs in human cells, which can change the information transmission from DNA to RNA and proteins. Most previous studies have focused on editing of the mRNAs. Here we reported that several kinds of RNAs, including miRNA, rRNA,
Chen, Huiping +4 more
core +1 more source
Nature Communications, 2023 Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration and generation of unintended mutations ...
Sandra Wimberger +27 more
doaj +1 more source
Sequence-Based Mapping and Genome Editing Reveal Mutations in Stickleback Hps5 Cause Oculocutaneous Albinism and the casper Phenotype. [PDF]
, 2017 Here, we present and characterize the spontaneous X-linked recessive mutation casper, which causes oculocutaneous albinism in threespine sticklebacks (Gasterosteus aculeatus).
Hart, James C, Miller, Craig T
core +2 more sources
Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]
, 2020 CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V +5 more
core
Non-Homologous End Joining and Homology Directed DNA Repair Frequency of Double-Stranded Breaks Introduced by Genome Editing Reagents. [PDF]
PLoS ONE, 2017 Genome editing using transcription-activator like effector nucleases or RNA guided nucleases allows one to precisely engineer desired changes within a given target sequence.
Michail Zaboikin +3 more
doaj +1 more source
The p53 challenge of hematopoietic stem cell gene editing
Molecular Therapy: Methods & Clinical Development, 2023 Ex vivo gene editing in hematopoietic stem and progenitor cells (HSPCs) represents a promising curative treatment strategy for monogenic blood disorders.
Sofie R. Dorset, Rasmus O. Bak
doaj +1 more source
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Human APOBEC1 cytidine deaminase edits HBV DNA [PDF]
Retrovirology, 2009 Abstract Retroviruses, hepadnaviruses, and some other retroelements are vulnerable to editing by single stranded DNA cytidine deaminases. Of the eleven human genes encoding such enzymes, eight have demonstrable enzymatic activity. Six of seven human APOBEC3 are able to hyperedit HBV DNA, frequently on both strands. Although human APOBEC1 (hA1)
Gonzalez, Minerva Cervantes +5 more
openaire +3 more sources