Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
Study of optimal method of Heterodera glycines sample preparation for molecular diagnostics
RUDN Journal of Agronomy and Animal IndustriesIn this study, it is optimized by the methods of preparing samples for the release of genetic material (DNA) from soybean cyst-forming nematode Heterodera glycines , which is necessary for accurate identification.
Anton V. Ivanov, Galina N. Bondarenko
doaj +1 more source
Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]
Iranian Journal of Animal Biosystematics, 2006 Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi +2 more
doaj +1 more source
California Fish and Wildlife Journal, 2023 Bighorn sheep (Ovis canadensis) habitat frequently is geographically discontinuous and the metapopulation model fits this species well. Consequently, extinction-colonization dynamics are important and need to be monitored.
John Wehausen
doaj +1 more source
Effectiveness of a novel extraction method for semen: comparison using liquid samples and dried stains [PDF]
, 2016 Forensic analysis of deoxyribonucleic acid (DNA) collected from sexual assault evidence is a multi-step process that requires a great amount of time and resources.
Cassis, Patricia Rose
core +1 more source
Impacts of DNA extraction and PCR on DNA metabarcoding estimates of soil biodiversity
Methods in Ecology and Evolution, 2018 Soils are ubiquitous and important components of terrestrial ecosystems, richly populated with diverse organisms with important ecological roles. DNA metabarcoding is a promising tool for efficient and taxonomically comprehensive analyses of soil ...
A. Dopheide +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
Modification of a commercial dna extraction kit to simultaneously recover rna, safely and rapidly, and to assess molecular biomass of the total and the active part of microbial communities, from soils with diverse mineralogy and carbon content : S11.04-P -15 [PDF]
, 2012 We have modified a commercial DNA extraction kit for soil to simultaneously co-extract RNA. In this new procedure RNA and DNA are separated by two selective purifications in cascade without the need of DNAase or RNAse digestion.
Amenc, Laurie +7 more
core +1 more source
FEBS Letters, EarlyView.In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi +5 more
wiley +1 more source
Monitoring genetic population biomarkers for wastewater-based epidemiology [PDF]
, 2017 We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic
Cooper, Jonathan M. +4 more
core +3 more sources