Results 91 to 100 of about 4,748,303 (367)

Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]

, 1993
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini, ASCENZIONI, Fiorentina, DONINI, Pierluigi, T. Gallo   +3 more
core   +1 more source

Gut Microbiota Analysis Results Are Highly Dependent on the 16S rRNA Gene Target Region, Whereas the Impact of DNA Extraction Is Minor.

Journal of Biomolecular Techniques, 2017
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As gut microbiota composition is a potential future target for clinical diagnostics, it is of utmost importance to enhance and optimize the NGS ...
Anniina Rintala, S. Pietilä, E. Munukka, E. Eerola, J. Pursiheimo, A. Laiho, S. Pekkala, P. Huovinen   +7 more
semanticscholar   +1 more source

A stepwise emergence of evolution in the RNA world

FEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients▿ [PDF]

, 2011
Limited data are available on the performance of different automated extraction platforms and commercially available quantitative real-time PCR (QRT-PCR) methods for the quantitation of cytomegalovirus (CMV) DNA in plasma.
Abbate, Allice, B. Munoz-Cobo, Bland, Boom, C. Solano, Caliendo, Caliendo, D. Bravo, D. Navarro, E. Costa, Espy, Gimeno, Gouarin, Gracia-Ahufinger, Hanson, Kerschner, M. A. Clari, M. Jose Remigia, Mengelle, Michelin, Miller, Pang, Pang, Piiparinen, Pillet, Pumannova, Razonable, Solano, the collaborative study group, Vincent, Wirgart, Yerly   +32 more
core   +2 more sources

Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities

PLoS ONE, 2017
The application of high-throughput, next-generation sequencing technologies has greatly improved our understanding of the human oral microbiome. While deciphering this diverse microbial community using such approaches is more accurate than traditional ...
A. Vesty, K. Biswas, Michael W Taylor, K. Gear, R. Douglas   +4 more
semanticscholar   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Estimating bacterial DNA synthesis from [3H] thymidine incorporation : discrepancies among macromolecular extraction procedures [PDF]

, 1991
The estimation of bacterial DNA synthesis in trophic studies with [3H] thymidine requires quantitative extraction of labeled DNA. To determine the DNA contribution to total macromolecular labeling in a eutrophic ecosystem, we tested three extraction ...
Bouvy, Marc, Torréton, Jean-Pascal
core   +1 more source

DNA nano-mechanics: how proteins deform the double helix [PDF]

, 2008
It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from its static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with proteins. We extract the local mean forces and torques acting on each base-pair of bound DNA from high-resolution
arxiv   +1 more source

Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota

Scientific Reports, 2019
The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS).
J. Rosenbaum, Mykhaylo Usyk, Zigui Chen, Christine P. Zolnik, H. Jones, L. Waldron, J. Dowd, L. Thorpe, R. Burk   +8 more
semanticscholar   +1 more source

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source