Results 101 to 110 of about 4,713,939 (389)
TRAF2 binds to TIFA via a novel motif and contributes to its autophagic degradation
FEBS Letters, EarlyView.TRAF family members couple receptor signalling complexes to downstream outputs, but how they interact with these complexes is not always clear. Here, we show that during ADP‐heptose signalling, TRAF2 binding to TIFA requires two short sequence motifs in the C‐terminal tail of TIFA, which are distinct from the TRAF6 binding motif.
Tom Snelling +4 more
wiley +1 more source
A simple and rapid DNA extraction method from leaves of grapevine suitable for polymerase chain reaction analysis. [PDF]
, 2012 The genomic grapevine (Vitis vinifera L.) DNA extraction is difficult because of secondary metabolites that interfere with DNA isolation procedures and subsequent applications.
ALFONZO, Antonio +5 more
core +1 more source
PLoS ONE, 2017 The application of high-throughput, next-generation sequencing technologies has greatly improved our understanding of the human oral microbiome. While deciphering this diverse microbial community using such approaches is more accurate than traditional ...
A. Vesty +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu +17 more
wiley +1 more source
Extraction and isolation of PCR amplifiable genomic DNA from Camellia sinensis (L.) Kuntze [PDF]
, 2011 Extraction and isolation of high quality DNA suitable for downstream molecular techniques in plants with high polyphenolic contents are almost impossible due to inhibitors of polymerase chain reactions.
Abdul Hamid, Azizah +4 more
core +1 more source
From omics to AI—mapping the pathogenic pathways in type 2 diabetes
FEBS Letters, EarlyView.Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan +2 more
wiley +1 more source
Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus [PDF]
, 2007 The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe.
BOONHAM, NEIL +9 more
core +1 more source
Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota
Scientific Reports, 2019 The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS).
J. Rosenbaum +8 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield +3 more
wiley +1 more source
DNA extraction for streamlined metagenomics of diverse environmental samples.
BioTechniques, 2017 A major bottleneck for metagenomic sequencing is rapid and efficient DNA extraction. Here, we compare the extraction efficiencies of three magnetic bead-based platforms (KingFisher, epMotion, and Tecan) to a standardized column-based extraction platform ...
C. Marotz +5 more
semanticscholar +1 more source