Determinants of cyclization-decyclization kinetics of short DNA with sticky ends [PDF]
arXiv, 2018 Cyclization of DNA with sticky ends is commonly used to construct DNA minicircles and to measure DNA bendability. The cyclization probability of short DNA (< 150 bp) has a strong length dependence, but how it depends on the rotational positioning of the sticky ends around the helical axis is less clear.
arxiv
DNA extraction: High yield is not synonymous with high diversity
Bioingeniøren, 2017 The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone +2 more
doaj
Impacts of DNA extraction and PCR on DNA metabarcoding estimates of soil biodiversity
Methods in Ecology and Evolution, 2018 Soils are ubiquitous and important components of terrestrial ecosystems, richly populated with diverse organisms with important ecological roles. DNA metabarcoding is a promising tool for efficient and taxonomically comprehensive analyses of soil ...
A. Dopheide +4 more
semanticscholar +1 more source
Evolutionary interplay between viruses and R‐loops
FEBS Letters, EarlyView.Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi +4 more
wiley +1 more source
Modification of a commercial dna extraction kit to simultaneously recover rna, safely and rapidly, and to assess molecular biomass of the total and the active part of microbial communities, from soils with diverse mineralogy and carbon content : S11.04-P -15 [PDF]
, 2012 We have modified a commercial DNA extraction kit for soil to simultaneously co-extract RNA. In this new procedure RNA and DNA are separated by two selective purifications in cascade without the need of DNAase or RNAse digestion.
Amenc, Laurie +7 more
core +1 more source
Pyrimido[1,2-a]-purin-10(3H)-one, M(1)G, is less prone to artifact than base oxidation [PDF]
, 2005 Pyrimido[1,2-a]-purin-10(3H)-one (M(1)G) is a secondary DNA damage product arising from primary reactive oxygen species (ROS) damage to membrane lipids or deoxyribose.
Jeong, Yo-Chan +3 more
core +3 more sources
Transverse tunneling current through guanine traps in DNA [PDF]
, 2005 The current - voltage dependence of the transverse tunneling current through the electron or hole traps in a DNA is investigated. The hopping of the charge between the sites of the trap and the charge-phonon coupling results in a staircase structure of the I-V curve.
arxiv +1 more source
Electronic Journal of Plant Breeding, 2017 Plant growth as well as productivity of rice (Oryza sativa L.), the major cereal and staple food of millions, is significantly affected by numerous biotic and abiotic stresses.
Shaheen A. Salam +3 more
doaj +1 more source
The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review.
Journal of Food Science, 2018 High-quality DNA extracts are imperative for downstream applications in molecular identification. Most processed food products undergo heat treatments causing DNA degradation, which hampers application of DNA-based techniques for food authentication ...
N. Sajali +5 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.The number of circulating tumor cells obtained from prostate cancer patients was increased approximately 5‐fold compared to regular CellSearch when processing 2 mL diagnostic leukapheresis material aliquots and increased by 44‐fold when processing 20 mL DLA aliquots using the flow enrichment target capture Halbach‐array.
Michiel Stevens +8 more
wiley +1 more source