Results 111 to 120 of about 4,713,939 (389)
Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice
FEBS Letters, EarlyView.In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász +6 more
wiley +1 more source
The Journal of Poultry Science, 2008 The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR).
Santosh Haunshi +4 more
doaj +1 more source
Evaluation of plant seed DNA and botanical evidence for potential forensic applications
Forensic Sciences Research, 2020 Seeds, the reproductive organs of plants, are common as trace evidence from crime scenes. Seed evidence could be grouped into several categories based on the types of crimes they are associated with, including child abuse, homicides and drugs.
Cheng-Lung Lee +3 more
doaj +1 more source
Environmental DNA, 2019 High-throughput DNA sequencing offers an efficient tool for assessing the taxonomic content of bulk arthropod samples. Many current DNA extraction protocols however require extensive handling of samples, like specimen-based DNA extractions, or sorting of
M. Nielsen +3 more
semanticscholar +1 more source
Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution
FEBS Letters, EarlyView.The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai +2 more
wiley +1 more source
Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus [PDF]
, 1994 Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin ...
Baker, A. +4 more
core
Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens [PDF]
, 1994 Discrimination of pathogenic and nonpathogenic Entamoeba histolytica is of great clinical importance. A simple and rapid DNA extraction method that can be used directly with stool specimens was developed without the need for prior cultivation of the ...
Katzwinkel-Wladarsch, S. +2 more
core +1 more source
Optimizing bacterial DNA extraction in urine
PLOS ONE, 2019 Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections. However, low quantities of bacterial DNA and PCR inhibitors in urine may prevent efficient PCR amplification for molecular detection of bacteria.
Laura C Chambers +7 more
openaire +5 more sources
Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells
FEBS Letters, EarlyView.Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan +2 more
wiley +1 more source
DNA extraction: High yield is not synonymous with high diversity
Bioingeniøren, 2017 The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone +2 more
doaj