Results 111 to 120 of about 4,873,130 (388)
, 2022 DNA extraction from frozen blood clots is challenging. Here we applied QIAGEN Clotspin Baskets and the Gentra Puregene Blood Kit for DNA extraction from 5.5 ml whole blood without anticoagulating additives.
openaire +1 more source
A Rapid Bacteriophage DNA Extraction Method
Methods and Protocols, 2018 Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria.
Džiuginta Jakočiūnė, A. Moodley
semanticscholar +1 more source
Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source
DNA extraction: High yield is not synonymous with high diversity
Bioingeniøren, 2017 The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone +2 more
doaj
Extraction and isolation of PCR amplifiable genomic DNA from Camellia sinensis (L.) Kuntze [PDF]
, 2011 Extraction and isolation of high quality DNA suitable for downstream molecular techniques in plants with high polyphenolic contents are almost impossible due to inhibitors of polymerase chain reactions.
Abdul Hamid, Azizah +4 more
core +1 more source
Environmental DNA, 2019 High-throughput DNA sequencing offers an efficient tool for assessing the taxonomic content of bulk arthropod samples. Many current DNA extraction protocols however require extensive handling of samples, like specimen-based DNA extractions, or sorting of
M. Nielsen +3 more
semanticscholar +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
Narrative Review of Bacterial DNA Extraction: Key Points and Challenges [PDF]
SINAI International Scientific JournalExtraction of bacterial DNA is a critical step in obtaining high-quality DNA. The overall quality, yield, and integrity of the genomic DNA isolated affects the success of any downstream applications.
Samar Solyman, Manar El Samak
doaj +1 more source
Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types
Metabarcoding and Metagenomics, 2018 Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed.
G. Sellers +3 more
semanticscholar +1 more source
FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source