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Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least ...
Ala Amiri+3 more
doaj
Evaluation of plant seed DNA and botanical evidence for potential forensic applications
Seeds, the reproductive organs of plants, are common as trace evidence from crime scenes. Seed evidence could be grouped into several categories based on the types of crimes they are associated with, including child abuse, homicides and drugs.
Cheng-Lung Lee+3 more
doaj +1 more source
An efficient method for DNA extraction from Cladosporioid fungi [PDF]
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A.+3 more
core +2 more sources
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis ...
F. Teng+7 more
semanticscholar +1 more source
Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore+8 more
core +3 more sources
Classification of large DNA methylation datasets for identifying cancer drivers [PDF]
DNA methylation is a well-studied genetic modification crucial to regulate the functioning of the genome. Its alterations play an important role in tumorigenesis and tumor-suppression. Thus, studying DNA methylation data may help biomarker discovery in cancer.
arxiv +1 more source
A crude method of DNA extraction and identification from exfoliated human buccal mucosa cells
Background: DNA analysis has a key role in forensic dentistry. However, techniques of DNA extraction and analysis are far from the reach of majority of medical professionals owing to its expensive set up. Aim: The present study was aimed at formulating a
Priyanka Kardam+4 more
doaj +1 more source
DNA Extraction and Polymerase Chain Reaction
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
N. Gupta
semanticscholar +1 more source
Evaluation of pre-analytical factors affecting plasma DNA analysis. [PDF]
Pre-analytical factors can significantly affect circulating cell-free DNA (cfDNA) analysis. However, there are few robust methods to rapidly assess sample quality and the impact of pre-analytical processing. To address this gap and to evaluate effects of
Berens, Michael E+15 more
core +1 more source
Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat ...
Farida Dwi Handayani+3 more
doaj +1 more source