Results 51 to 60 of about 846,282 (296)

Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 inbanana

open access: yesBiotecnología Vegetal, 2021
Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen.
María I. Oloriz   +4 more
doaj  

Determining the optimal method for DNA isolation from fruit jams

open access: yesCzech Journal of Food Sciences, 2018
DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances.
Tereza SOVOVÁ   +2 more
doaj   +1 more source

An improved method for extraction of microbial DNA from alkaline-saline soil

open access: yesTerra Latinoamericana, 2021
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández   +9 more
doaj   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

open access: yesFEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion

open access: yes대한환경공학회지, 2021
Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
doaj   +1 more source

Single cis‐elements in brassinosteroid‐induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA‐binding domain

open access: yesFEBS Letters, EarlyView.
Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki   +4 more
wiley   +1 more source

Lysis Buffers for Detecting Pork Content in Food Products

open access: yesIndonesian Journal of Chemistry
The increasing number of counterfeit processed food products with non-halal ingredients, such as pork, has caused public concern about the halalness of Indonesian food products, especially in the Muslim community.
Ulfah Lailatul Khoiriah   +5 more
doaj   +1 more source

Evaluation of plant seed DNA and botanical evidence for potential forensic applications

open access: yesForensic Sciences Research, 2020
Seeds, the reproductive organs of plants, are common as trace evidence from crime scenes. Seed evidence could be grouped into several categories based on the types of crimes they are associated with, including child abuse, homicides and drugs.
Cheng-Lung Lee   +3 more
doaj   +1 more source

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

open access: yesFEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican   +7 more
wiley   +1 more source

DNA extraction: High yield is not synonymous with high diversity

open access: yesBioingeniøren, 2017
The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone   +2 more
doaj  

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