Results 51 to 60 of about 4,713,939 (389)

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction

open access: yesBMC Infectious Diseases, 2019
BackgroundHepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.MethodsHere we ...
X. Shen   +10 more
semanticscholar   +1 more source

An improved method for extraction of microbial DNA from alkaline-saline soil

open access: yesTerra Latinoamericana, 2021
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández   +9 more
doaj   +1 more source

DNA extraction for human microbiome studies: the issue of standardization

open access: yesGenome Biology, 2019
Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions.
K. Greathouse, R. Sinha, E. Vogtmann
semanticscholar   +1 more source

DNA Extraction Systematics for Spectroscopic Studies [PDF]

open access: yesSensors, 2008
Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a ...
Palma, Bianca Fogazza   +5 more
openaire   +4 more sources

Molecular characterization of rice (Oryza sativa L.) genotypes for drought tolerance using two SSR markers

open access: yesElectronic Journal of Plant Breeding, 2017
Plant growth as well as productivity of rice (Oryza sativa L.), the major cereal and staple food of millions, is significantly affected by numerous biotic and abiotic stresses.
Shaheen A. Salam   +3 more
doaj   +1 more source

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2014
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj   +1 more source

Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling

open access: yesScientific Reports, 2018
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis ...
F. Teng   +7 more
semanticscholar   +1 more source

Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion

open access: yes대한환경공학회지, 2021
Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
doaj   +1 more source

Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]

open access: yes, 1993
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini   +3 more
core   +1 more source

Comparison and Optimization of Ancient DNA Extraction [PDF]

open access: yesBioTechniques, 2007
Ancient DNA analyses rely on the extraction of the tiny amounts of DNA remaining in samples that are hundreds to tens of thousands of years old. Despite the critical role extraction efficiency plays in this field of research, no study has comprehensively compared ancient DNA extraction techniques to date.
Michael Hofreiter, Nadin Rohland
openaire   +4 more sources

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