Results 51 to 60 of about 4,713,939 (389)
BackgroundHepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.MethodsHere we ...
X. Shen+10 more
semanticscholar +1 more source
An improved method for extraction of microbial DNA from alkaline-saline soil
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández+9 more
doaj +1 more source
DNA extraction for human microbiome studies: the issue of standardization
Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions.
K. Greathouse, R. Sinha, E. Vogtmann
semanticscholar +1 more source
DNA Extraction Systematics for Spectroscopic Studies [PDF]
Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a ...
Palma, Bianca Fogazza+5 more
openaire +4 more sources
Plant growth as well as productivity of rice (Oryza sativa L.), the major cereal and staple food of millions, is significantly affected by numerous biotic and abiotic stresses.
Shaheen A. Salam+3 more
doaj +1 more source
Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj +1 more source
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis ...
F. Teng+7 more
semanticscholar +1 more source
Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion
Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
doaj +1 more source
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini+3 more
core +1 more source
Comparison and Optimization of Ancient DNA Extraction [PDF]
Ancient DNA analyses rely on the extraction of the tiny amounts of DNA remaining in samples that are hundreds to tens of thousands of years old. Despite the critical role extraction efficiency plays in this field of research, no study has comprehensively compared ancient DNA extraction techniques to date.
Michael Hofreiter, Nadin Rohland
openaire +4 more sources