Results 121 to 130 of about 1,932,999 (290)
DNA fragmentation in placenta of infected mice.
, 2013 A: Placenta of P. berghei infected mice showing distinct DNA fragmentation on day 4 and 6 PI respectively compared with DNA of normal placenta. B: No DNA fragmentation observed in blood from infected and normal mouse.
Geeta Shukla (330926) +2 more
core +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Fragmentation of Organic Complexes Under Mass Spectrometry
, 2022 In the thesis, fragmentation of the metallic, organic complexes were discussed and studied under mass spectrometry. We utilized the Bruker software to identify all the compounds and their fragments.
Aliyeva, Zenfira
core
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Association of age with sperm DNA fragmentation [PDF]
, 2011 Artículo de publicación ISIBackground: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA.
Smith Garcés, Rosita +4 more
core
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Sperm DNA fragmentation and male subfertility
, 2012 The aims of the present study were to investigate whether: 1. There is a difference in sperm DNA fragmentation between subfertile men and fertile controls. 2. Sperm DNA fragmentation differs among men with different causes of subfertility. 3.
Κανταρτζή, Περσεφόνη-Δήμητρα +1 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source