Results 101 to 110 of about 222,504 (300)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
The role of oxidative stress in male infertility: Connection to sperm telomere shortening and overall DNA damage [PDF]
Arhiv za farmacijuMale infertility affects approximately 20% of men, with 30-40% of cases being linked to issues in both partners. While sperm production may be normal, DNA damage in spermatozoa can occur and become a primary cause of infertility.
Glišić Biljana +1 more
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
DNA fragmentation and morphological changes in apoptotic human lymphocytes
, 2002 Cell suspensions enriched in cells at various stages of apoptosis were obtained by separation of irradiated human peripheral blood lymphocytes on density gradients at different post-irradiation times.
Elisabetta Testa +10 more
core +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
, 2016 Spermcompetition, a prevalent evolutionary process inwhich the spermatozoa of two or more males compete for the fertilization of the same ovum, leads to morphological and physiological adaptations, including increases in energetic metabolism that may ...
Soler, Ana J. +7 more
core +1 more source
Cryopreservation increases DNA fragmentation in spermatozoa of smokers
, 2013 Smoking causes subfertility due to deterioration of spermatozoa including decreased concentration and abnormal morphology. Although evidence on the deleterious effects of smoking on spermatozoa parameters is well known, its interference with ...
Mehmet Serif Aydin +5 more
core +2 more sources
Анализ связи фрагментации ДНК и анеуплоидий спермы с параметрами ранних эмбрионов
Фактори експериментальної еволюції організмів, 2016 Aim. The dependence of the process of blastocyst formation in IVF practice on the level of sperm DNA fragmentation and sperm aneuploidies in men with low reproductive function was studied. Methods.
Е. С. Жилкова +4 more
doaj
SPERM DNA FRAGMENTATION IN INFERTILE MEN OF KUZBASS
Мать и дитя в Кузбассе, 2022 The standard method for diagnosing male infertility involves a spermogram, which does not always give a complete picture of male fertility. One additional method for assessing ejaculate is the sperm DNA fragmentation test (SDF), which determines the ...
Алексей Геннадьевич Тришкин +10 more
doaj