Results 81 to 90 of about 222,504 (300)
Mutant DNA quantification by digital PCR can be confounded by heating during DNA fragmentation
BioTechniques, 2016 Digital PCR (dPCR) is gaining popularity as a DNA mutation quantification method for clinical specimens. Fragmentation prior to dPCR is required for non-fragmented genomic DNA samples; however, the effect of fragmentation on DNA analysis has not been ...
Qing Kang +3 more
doaj +1 more source
Molecular Oncology, EarlyView.Circulating tumor cells (CTCs) and plasma cell‐free DNA (cfDNA) were analyzed to detect ESR1 mutations and methylation in patients with advanced breast cancer. CTC‐derived DNA showed higher sensitivity for mutation detection and revealed complementary genetic and epigenetic alterations, highlighting the added value of CTC analysis for understanding ...
Dimitra Stergiopoulou +12 more
wiley +1 more source
Apoptotic nuclear morphological change without DNA fragmentation
, 1999 Apoptosis is characterized morphologically by condensation and fragmentationof nuclei and cells and biochemically by fragmentation of chromosomal DNA into nucleosomal units [1].
Enari, Masato +9 more
core +1 more source
Research and Reports in Urology, 2021 Minh Tam Le,1,2 Trung Van Nguyen,1 Thai Thanh Thi Nguyen,1 Hiep Tuyet Thi Nguyen,1 Duong Dinh Le,3 Vu Quoc Huy Nguyen2 1Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam ...
Le MT +5 more
doaj
PLoS ONE, 2018 DNA methylation is one of the most important epigenetic modifications in the regulation of gene transcription. The current gold standard to study this modification is bisulfite sequencing.
Sam Kint +4 more
doaj +1 more source
Interpreting the effects of DNA polymerase variants at the structural level
Molecular Oncology, EarlyView.Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi +7 more
wiley +1 more source
DNA fragmentation analysis in frozen thawed spermatozoa.
, 2016 DNA fragmentation level was measured by the sperm chromatin dispersion (SCD) assay in the processed fraction at 0 (N = 32) (□) and 6h (N = 32) (■). Please note that fold change in the sperm DNA fragmentation level with corresponding interval of EA were ...
Fiona D’Souza (2589751) +8 more
core +1 more source
Stacking interactions in denaturation of DNA fragments [PDF]
The European Physical Journal E, 2011 A mesoscopic model for heterogeneous DNA denaturation is developed in the framework of the path integral formalism. The base pair stretchings are treated as one-dimensional, time dependent paths contributing to the partition function. The size of the paths ensemble, which measures the degree of cooperativity of the system, is computed versus ...
openaire +4 more sources
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
, 2008 Morfológiai és biokémiai vizsgálataink arra utalnak, hogy a a genotoxikus hatások kategorizálhatók az okozott kromatin változások alapján. A kemotoxikus változások potenciális diagnosztikus jelentősége miatt vizsgáltuk a nehézfémek (elsősosrban kadmium) (
Szepessy, Edit +6 more
core