Results 91 to 100 of about 373,852 (297)
Mutant DNA quantification by digital PCR can be confounded by heating during DNA fragmentation
BioTechniques, 2016 Digital PCR (dPCR) is gaining popularity as a DNA mutation quantification method for clinical specimens. Fragmentation prior to dPCR is required for non-fragmented genomic DNA samples; however, the effect of fragmentation on DNA analysis has not been ...
Qing Kang +3 more
doaj +1 more source
Distribution and genetic variability of Staphylinidae across a gradient of anthropogenically influenced insular landscapes [PDF]
, 2016 This paper describes the distribution and genetic variability of rove beetles (Coleoptera Staphylinidae) in anthropogenically influenced insular landscapes.
Borges, Paulo A. V. +5 more
core
RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]
, 2013 Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee +5 more
core +3 more sources
Molecular Oncology, EarlyView.Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen +11 more
wiley +1 more source
Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy [PDF]
, 2017 Radiotherapy is the standard treatment for head and neck squamous cell carcinoma (HNSCC), however, radioresistance remains a major clinical problem despite significant improvements in treatment protocols. Therapeutic outcome could potentially be improved
Beavis, Andrew W. +5 more
core +1 more source
Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer
Molecular Oncology, EarlyView.COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann +61 more
wiley +1 more source
Research and Reports in Urology, 2021 Minh Tam Le,1,2 Trung Van Nguyen,1 Thai Thanh Thi Nguyen,1 Hiep Tuyet Thi Nguyen,1 Duong Dinh Le,3 Vu Quoc Huy Nguyen2 1Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam ...
Le MT +5 more
doaj
Evidence for survival of Pleistocene climatic changes in Northern refugia by the land snail Trochoidea geyeri (Soós 1926) (Helicellinae, Stylommatophora) [PDF]
, 2003 The study of organisms with restricted dispersal abilities and presence in the fossil record is particularly adequate to understand the impact of climate changes on the distribution and genetic structure of species.
Pfenninger, Markus +2 more
core +1 more source
Molecular Oncology, EarlyView.Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch +13 more
wiley +1 more source
Advances in Urology, 2015 We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study.
Essam-Elden M. Mohammed +5 more
doaj +1 more source