Results 131 to 140 of about 43,780 (231)

The ‘very moment’ when UDG recognizes a flipped-out uracil base in dsDNA

Scientific Reports
Uracil-DNA glycosylase (UDG) is the first enzyme in the base-excision repair (BER) pathway, acting on uracil bases in DNA. How UDG finds its targets has not been conclusively resolved yet.
Vinnarasi Saravanan, Nessim Raouraoua, Guillaume Brysbaert, Stefano Giordano, Marc F. Lensink, Fabrizio Cleri, Ralf Blossey   +6 more
doaj   +1 more source

Molecular biology techniques as a tool for detection and characterisation of Mycobacterium avium subsp. paratuberculosis [PDF]

, 2002
Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) is the causative agent of paratuberculosis, also known as Johne’s disease, a chronic intestinal infection in cattle and other ruminants.
Englund, Stina
core  

Demethylation of DNA by purified chick embryo 5-methylcytosine-DNA glycosylase requires both protein and RNA [PDF]

, 2017
We have previously purified and characterized a 5-methylcytosine (5-MeC)-DNA glycosylase from 12 day old chick embryos [Jost,J.P. et al. (1995) J. Biol. Chem. 270, 9734-9739].
Frémont, Marc, Gaulis, Swann, Hess, Daniel, Jost, Jean-Pierre, Matthies, Renate, Siegmann, Michel   +5 more
core  

Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

Nature Communications
DNA base editors enable direct editing of adenine (A), cytosine (C), or guanine (G), but there is no base editor for direct thymine (T) editing currently. Here we develop two deaminase-free glycosylase-based base editors for direct T editing (gTBE) and C
Huawei Tong, Haoqiang Wang, Xuchen Wang, Nana Liu, Guoling Li, Danni Wu, Yun Li, Ming Jin, Hengbin Li, Yinghui Wei, Tong Li, Yuan Yuan, Linyu Shi, Xuan Yao, Yingsi Zhou, Hui Yang   +15 more
doaj   +1 more source

The kinetics of uracil-N-glycosylase distribution inside replication foci

Scientific Reports
Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in ...
Anna Ligasová, Ivo Frydrych, Barbora Piskláková, David Friedecký, Karel Koberna   +4 more
doaj   +1 more source

Efficient glycosylase-mediated base editing with minimal off-target effects in mammalian embryos

Genome Biology
Developing glycosylase-based base editors (gBEs) to broaden the editing scope is highly desirable for biomedical research and agricultural applications. However, the off-target effects and applicability of gBEs need further investigation.
Yinghui Wei, Xi Cao, Shuhong Huang, Yang Yue, Muhua Luo, Zujiang Liu, Bo Liu, Qijing Zhang, Yangsheng Wu, Liqin Wang, Jiapeng Lin, Yulin Chen, Wenxin Zheng, Weiwei Wu, Kun Xu, Xiaolong Wang   +15 more
doaj   +1 more source

Uracil-DNA Glycosylase [PDF]

, 2020
openaire   +1 more source

Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression. [PDF]

Cancer Cell Int
Yang JJ, Shi LZ, Hu YW, Chen H, Wang QH, Han W.   +5 more
europepmc   +1 more source

A monofunctional-like mutant of DNA glycosylase NTHL1 changes the dynamics of DNA repair during acute oxidative stress. [PDF]

J Biol Chem
Haslam J, Rudolfova N, Zhou K, Homan E, Jemth AS, Michel M, Helleday T, Mortusewicz O.   +7 more
europepmc   +1 more source

Uracil—DNA glycosylase from Bacillus stearothermophilus [PDF]

FEBS Letters, 1981
Kaboev, Oleg K., Luchkina, Ludmila A., Akhmedov, Alexander T., Bekker, Michael L.   +3 more
openaire   +2 more sources