Results 61 to 70 of about 2,038,987 (238)

DNA Sequence Context Effects on the Glycosylase Activity of Human 8-Oxoguanine DNA Glycosylase [PDF]

Journal of Biological Chemistry, 2012
Human 8-oxoguanine DNA glycosylase (OGG1) is a key enzyme involved in removing 7,8-dihydro-8-oxoguanine (8-oxoG), a highly mutagenic DNA lesion generated by oxidative stress. The removal of 8-oxoG by OGG1 is affected by the local DNA sequence, and this feature most likely contributes to observed mutational hot spots in genomic DNA.
Akira, Sassa, William A, Beard, Rajendra, Prasad, Samuel H, Wilson   +3 more
openaire   +2 more sources

Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application

Drug Testing and Analysis, EarlyView.
This article presents an optimized protocol for gene doping detection. Starting from one spot DBS (20 μL), concentrated DNA was obtained and real‐time PCR parameters were adjusted for increased detection. Robust detection of 5000 copies/mL of transgene was validated for polymeric device TASSO M‐20.
Alexandre Marchand, Ingrid Roulland, Magnus Ericsson   +2 more
wiley   +1 more source

Characterization of demethylating DNA glycosylase ROS1 from Nicotiana tabacum L.

Вавиловский журнал генетики и селекции, 2022
One of the main mechanisms of epigenetic regulation in higher eukaryotes is based on the methylation of cytosine at the C5 position with the formation of 5-methylcytosine (mC), which is further recognized by regulatory proteins.
D. V. Petrova, N. V. Permyakova, I. R. Grin, D. O. Zharkov   +3 more
doaj   +1 more source

Molecular crowding enhances facilitated diffusion of two human DNA glycosylases

Nucleic Acids Research, 2015
Intracellular space is at a premium due to the high concentrations of biomolecules and is expected to have a fundamental effect on how large macromolecules move in the cell.
Shannen L. Cravens, J. Schonhoft, Meng M. Rowland, Alyssa A. Rodriguez, Breeana G. Anderson, J. Stivers   +5 more
semanticscholar   +1 more source

Epigenotoxicity: Decoding the epigenetic imprints of genotoxic agents and their implications for regulatory genetic toxicology

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Regulatory genetic toxicology focuses on DNA damage and subsequent gene mutations. However, genotoxic agents can also affect epigenetic marks, and incorporation of epigenetic data into the regulatory framework may thus enhance the accuracy of risk assessment.
Roger Godschalk, Christopher Faulk, Jessica LaRocca, Jan van Benthem, Francesco Marchetti   +4 more
wiley   +1 more source

Uracil-DNA glycosylases preferentially excise mispaired uracil [PDF]

Biochemical Journal, 1992
We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the 5′-32P-labelled strand an uracil residue facing, on the complementary strand, guanine (mimicking cytosine deamination) or adenine (mimicking dUTP ...
VERRI A, MAZZARELLO P, SPADARI S, FOCHER F   +3 more
openaire   +3 more sources

Bridging the Gap Between Human Toxicology and Ecotoxicology Under One Health Perspective by a Cross‐Species Adverse Outcome Pathway Network for Reproductive Toxicity

Environmental Toxicology and Chemistry, EarlyView.
Cross‐species extrapolation of adverse outcome pathway network on reproductive toxicity under the One Health perspective using new approach methodologies. AOP = adverse outcome pathway. Abstract Although ecotoxicological and toxicological risk assessments are performed separately from each other, recent efforts have been made in both disciplines to ...
Elizabeth Dufourcq Sekatcheff, Jaeseong Jeong, Jinhee Choi   +2 more
wiley   +1 more source

Identification of Escherichia coli Mismatch-specific Uracil DNA Glycosylase as a Robust Xanthine DNA Glycosylase [PDF]

Journal of Biological Chemistry, 2010
The gene for the mismatch-specific uracil DNA glycosylase (MUG) was identified in the Escherichia coli genome as a sequence homolog of the human thymine DNA glycosylase with activity against mismatched uracil base pairs. Examination of cell extracts led us to detect a previously unknown xanthine DNA glycosylase (XDG) activity in E. coli.
Hyun-Wook, Lee, Allyn R, Brice, Charles B, Wright, Brian N, Dominy, Weiguo, Cao   +4 more
openaire   +2 more sources

Epigenetic Regulation in the Pathogenesis of Renal Inflammation: Insights and Therapeutic Potentials

iNew Medicine, EarlyView.
ABSTRACT Renal inflammation is a common pathological process in various kidney diseases, often initiated by factors such as toxins, ischemia, or autoimmune reactions. This inflammatory response can result in structural damage and a rapid decline in renal function.
Yu‐Hang Dong, Xiao‐Yu Shen, Han‐Cheng Jin, Xiang‐Yu Li, Hao He, Xiao‐Ming Meng   +5 more
wiley   +1 more source

5-Methylcytosine Recognition by Arabidopsis thaliana DNA Glycosylases DEMETER and DML3

Biochemistry, 2014
Methylation of cytosine to 5-methylcytosine (5mC) is important for gene expression, gene imprinting, X-chromosome inactivation, and transposon silencing.
Sonja C Brooks, R. Fischer, J. Huh, B. Eichman   +3 more
semanticscholar   +1 more source