Results 1 to 10 of about 2,150 (122)

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering [PDF]

, 2014
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian ...
A Aronheim, Anne Mathilde Lund, BG Hansen, Bjarne Gram Hansen, C Aslanidis, D Kalderon, DG Gibson, F Geu-Flores, FM Wurm, G Walsh, G Walsh, Helene Faustrup Kildegaard, HH Nour-Eldin, HH Nour-Eldin, J Bitinaite, J Burke, J Roth, JF Hancock, Julie Rank, L Fliegel, Maja Borup Kjær Petersen, Mark Isalan, MH Nørholm, Mikael Rørdam Andersen, MY Zhou, NE Lewis, PW Gray, PW Gray, R Rizzuto, S Munro, S Munro, SJ Gould, SS Tate, T Ellis, T Smith, Uffe Hasbro Mortensen, X Xu, Y Durocher   +37 more
core   +8 more sources

The Verrucomicrobia LexA-binding Motif: Insights into the Evolutionary Dynamics of the SOS Response

Frontiers in Molecular Biosciences, 2016
The SOS response is the primary bacterial mechanism to address DNA damage, coordinating multiple cellular processes that include DNA repair, cell division and translesion synthesis.
Ivan Erill, Susana Campoy, Sefa Kılıç, Jordi Barbé   +3 more
doaj   +1 more source

Structure and stereochemistry of the base excision repair glycosylase MutY reveal a mechanism similar to retaining glycosidases. [PDF]

, 2015
MutY adenine glycosylases prevent DNA mutations by excising adenine from promutagenic 8-oxo-7,8-dihydroguanine (OG):A mismatches. Here, we describe structural features of the MutY active site bound to an azaribose transition state analog which indicate a
Cao, Sheng, Chu, Aurea, David, Sheila S, Horvath, Martin P, O'Shea, Valerie L, Richards, Jody L, Woods, Ryan D   +6 more
core   +2 more sources

Human uracil DNA N-glycosidase: studies in normal and repair defective cultured fibroblasts

Nucleic Acids Research, 1978
Uracil DNA N-glycosidase, an enzyme which participates in the excision of uracil from DNA, was measured in extracts from fibroblasts lines cultured from normal subjects, from several subjects with the genetic disease xeroderma pigmentosum, and from a subject with ataxia telangiectasia.
U, Kuhnlein, B, Lee, S, Linn
openaire   +3 more sources

Removal of Misincorporated Ribonucleotides from Prokaryotic Genomes: An Unexpected Role for Nucleotide Excision Repair [PDF]

, 2013
Stringent steric exclusion mechanisms limit the misincorporation of ribonucleotides by high-fidelity DNA polymerases into genomic DNA. In contrast, low-fidelity Escherichia coli DNA polymerase V (pol V) has relatively poor sugar discrimination and ...
A Vaisman, AL Lu, Alexandra Vaisman, AR Fernández de Henestrosa, B Hiller, B Rydberg, B Van Houten, BD Davis, Bennett Van Houten, D Mu, DJ Crowley, DL Croteau, DL Croteau, DM Maron, Donald Huston, E Curti, E Wolff, EA Kouzminova, EF DeRose, EG Frank, EG Frank, EW Hou, F Lazzaro, G Churchward, G Jiang, GF Moolenaar, H Krokan, H Ohmori, H Shinagawa, H Wang, IJ Fijalkowska, J Yang, JA Brown, JB Sweasy, JC Huang, JE Sale, JG Moggs, JJ Lin, JJ Truglio, JL Sparks, John P. McDonald, JP McDonald, K Sugasawa, L Garibyan, L Zheng, LA Christensen, Lili Liu, M Sandigursky, M Skorvaga, M Tang, M Watanabe-Akanuma, M Yamaguchi, MA Reijns, MC Wahl, ME Branum, MM Ghodgaonkar, MR Albertella, N Kim, Q Jiang, Q Ruan, R Cone, R Woodgate, RJ Sanderson, Roger Woodgate, RP Cunningham, SA Lujan, SA Nick McElhinny, SA Nick McElhinny, Sue Jinks-Robertson, T Baba, T Lindahl, T Lindahl, T Nakano, W Kuban, W Yang, Wojciech Kuban, Y Shen, YW Kow   +77 more
core   +3 more sources

Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications [PDF]

, 2016
The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important DNA element in diverse systems from specific phages to antibody maturation and Drosophila development.
Borsos, Máté, Békési, Angéla, Nagy, Kinga, Pálinkás, Hajnalka Laura, Róna, Gergely, Scheer, Ildikó, Tihanyi, Gergely, Vértessy, Beáta G.   +7 more
core   +1 more source

Enzymes from Micrococcus luteus involved in the initial steps of excision repair of spontaneous DNA lesions: uracil-DNA-glycosidase and apurinic-endonucleases

Nucleic Acids Research, 1978
Uracil-DNA-glycosidase that releases free uracil from single-stranded or double-stranded deaminated DNA and poly d(A-U) has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 16,000 and can be separated from uracil-endonuclease and endonucleases (AP-endonucleases) specific for apurinic and apyrimidinic sites ...
N V, Tomlin, O N, Aprelikova, L S, Barenfeld   +2 more
openaire   +3 more sources

The glycine-rich motif of Pyrococcus abyssi DNA polymerase D is critical for protein stability. [PDF]

, 2010
En libre-accès sur Archimer : http://archimer.ifremer.fr/doc/00002/11293/7879.pdfInternational audienceA glycine-rich motif described as being involved in human polymerase delta proliferating cell nuclear antigen (PCNA) binding has also been identified ...
Castrec, Benoît, Flament, Didier, Henneke, Ghislaine, Laurent, Sébastien, Raffin, Jean-Paul   +4 more
core   +2 more sources

A family of thermostable fungal cellulases created by structure-guided recombination [PDF]

, 2009
SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermostable CBH II chimeras.
A. Villalobos, C. D. Snow, C. Nguyen, F. H. Arnold, Go, Godbole, Hui, I. Wu, Innis, J. Minshull, Jeoh, Koivula, Li, Liu, Lussier, Nelson, Otey, P. Heinzelman, S. Govindarajan, Sch  lein   +19 more
core   +3 more sources

The isolation and characterisation of temperature-dependent Ricin A chain molecules in Saccharomyces cerevisiae. [PDF]

, 2007
Ricin is a heterodimeric plant protein that is potently toxic to mammalian cells. Toxicity results from the catalytic depurination of eukaryotic ribosomes by ricin A chain (RTA) that follows toxin endocytosis to, and translocation across, the ...
Allen, Brunger, Chaddock, Di Cola, Di Cola, Endo, Esnouf, Frankel, Gemmill, Gietz, Gould, Hazes, Herruer, Higuchi, Hoffman, Iordanov, Jolliffe, Jones, Kirby, Kraulis, Lamb, Li, Lord, Lord, Lord, Marsden, Moazed, Murshudov, Olsnes, Otwinowski, Perrakis, Rapak, Read, Ready, Sambrook, Schmitt, Sikorski, Simpson, Simpson, Wales, Wesche, Weston   +41 more
core   +1 more source