Results 11 to 20 of about 2,150 (122)

Integrated Chemoenzymatic Synthesis of the mRNA Vaccine Building Block N¹-Methylpseudouridine Triphosphate. [PDF]

Angew Chem Int Ed Engl
Pseudouridine‐5′‐triphosphate (ΨTP) and its N1‐methylated derivative (m1ΨTP) are crucial components in mRNA vaccine technology. Here, an atom‐economic, high‐yielding biocatalytic route for ΨTP synthesis is presented. A new chemoenzymatic route for m1ΨTP synthesis was developed.
Pfeiffer M, Krammer L, Zöhrer J, Breinbauer R, Nidetzky B.   +4 more
europepmc   +2 more sources

APOBEC3G protects the genome of human cultured cells and mice from radiation‐induced damage

The FEBS Journal, Volume 290, Issue 7, Page 1822-1839, April 2023., 2023
A3G protects mice from IR‐induced damage and promotes accurate DNA double‐strand breaks repair in cultured cells. Transgenic mice expressing A3G successfully survived lethal irradiation, whereas wild‐type controls quickly succumbed to radiation syndrome.
Yelena Britan‐Rosich, Jing Ma, Eran Kotler, Faizan Hassan, Alexander Botvinnik, Yoav Smith, Ofra Moshel, Abed Nasereddin, Gunjan Sharma, Eli Pikarsky, Susan Ross, Moshe Kotler   +11 more
wiley   +1 more source

Recent Advances in the Development and Synthesis of Carbohydrate‐Based Molecules with Promising Antibacterial Activity

European Journal of Organic Chemistry, Volume 26, Issue 4, January 24, 2023., 2023
In this review, the potential of carbohydrates as starting materials for the synthesis of antimicrobial agents is explored. The most recent and successful reports of sugar‐based bactericides, bacteriostatic agents, and adhesin binders are covered with focus on the methodologies employed in their synthesis, resulting in a useful compilation of state‐of ...
Ana Marta de Matos
wiley   +1 more source

Novel molecules and target genes for vegetative heat tolerance in wheat

Plant-Environment Interactions, Volume 3, Issue 6, Page 264-289, December 2022., 2022
Chronic heat stress induces distinct physiological, metabolomic, and transcriptomic changes in a heat‐tolerant Triticum aestivum cultivar, Cadenza, compared to a susceptible cultivar, Paragon. Previously uncharacterized metabolites and genes are differentially regulated in the heat‐tolerant cultivar, providing potential for future crop heat tolerance ...
Teresa Rose, Mark Wilkinson, Claudia Lowe, Jiemeng Xu, David Hughes, Kirsty L. Hassall, Keywan Hassani‐Pak, Sandeep Amberkar, Clarice Noleto‐Dias, Jane Ward, Sigrid Heuer   +10 more
wiley   +1 more source

OGG1 contributes to hepatocellular carcinoma by promoting cell cycle‐related protein expression and enhancing DNA oxidative damage repair in tumor cells

Journal of Clinical Laboratory Analysis, Volume 36, Issue 7, July 2022., 2022
Figure 1 Expression of OGG1 in HCC, (A) expression of OGG1 in nucleoplasma, (B, C) OGG1 expression in healthy liver tissues, (D, E, F) OGG1 expression in liver tissues of HCC patients (G) expression of OGG1 in HCC based on sample type (H) nodal metastasis status (I) TP53 mutation status (J) age (K) gender (L) race (M) stage (N) grade (O) histological ...
He Zhang, Peng‐jun Jiang, Meng‐yuan Lv, Yan‐hua Zhao, Ju Cui, Jie Chen   +5 more
wiley   +1 more source

Pharmacomicrobiomics: Influence of gut microbiota on drug and xenobiotic metabolism

The FASEB Journal, Volume 36, Issue 6, June 2022., 2022
Abstract Gut microbiota is the most diverse and complex biological ecosystem, which is estimated to consist of greater than 5 million distinct genes and 100 trillion cells which are in constant communication with the host environment. The interaction between the gut microbiota and drugs and other xenobiotic compounds is bidirectional, quite complicated,
Ifeoma Julieth Dikeocha, Abdelkodose Mohammed Al‐Kabsi, Muhammad Miftahussurur, Mohammed Abdullah Alshawsh   +3 more
wiley   +1 more source

Extracellular loops matter – subcellular location and function of the lysine transporter Lyp1 from Saccharomyces cerevisiae

The FEBS Journal, Volume 287, Issue 20, Page 4401-4414, October 2020., 2020
We performed alanine‐scanning mutagenesis of the extracellular loops of the yeast lysine transporter Lyp1. We find positions that affect either the localization or activity of Lyp1. Half of the affected mutants are located in the extension of extracellular loop 3 or in a predicted α‐helix in extracellular loop 4. We conclude that extracellular loops of
Joury S. van‘t Klooster, Frans Bianchi, Ruben B. Doorn, Mirco Lorenzon, Jarnick H. Lusseveld, Christiaan M. Punter, Bert Poolman   +6 more
wiley   +1 more source

Regio‐ and Stereoselective Steroid Hydroxylation at C7 by Cytochrome P450 Monooxygenase Mutants

Angewandte Chemie, Volume 132, Issue 30, Page 12599-12605, July 20, 2020., 2020
One mutant, six targets: The regio‐ and stereoselective C−H activation for hydroxylation of six different steroids with formation of C7β alcohols was accomplished using a single P450 mutant evolved by protein library construction based on a special DNA assembly and cloning procedure.
Aitao Li, Carlos G. Acevedo‐Rocha, Lorenzo D'Amore, Jinfeng Chen, Yaqin Peng, Marc Garcia‐Borràs, Chenghua Gao, Jinmei Zhu, Harry Rickerby, Sílvia Osuna, Jiahai Zhou, Manfred T. Reetz   +11 more
wiley   +1 more source

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells [PDF]

, 2015
Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non ...
Andersen, Mikael Rørdam, Grav, Lise Marie, Hansen, Henning Gram, Kildegaard, Helene Faustrup, Kol, Stefan, Lund, Anne Mathilde, Lundqvist, Magnus, Min Lee, Gyun, Nilsson, Claes Nymand, Rockberg, Johan   +9 more
core   +1 more source

Effects of novelly synthesized nucleolipides on different tumor cell lines (HT29, HepG2, Panc-1, RenCa) with special respect to glioma cell lines (BT4Ca, GOS3, G28, G112, U251, U87) of human or other species [PDF]

, 2020
Today cancer is the second leading cause of death around the world. The World Health Organization predicts an increase from 9.6 million in 2018 up to 16.4 million cancer deaths in 2040.
Hammerbacher, Katharina
core   +1 more source