Results 11 to 20 of about 1,466,903 (122)

Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase [PDF]

, 2023
DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of ...
Lou‐Hing, Anna, Ahmed, Nadia R., Hanson, Karl M., Buckley, Ryan J., Christopher D. O. Cooper, Edward L. Bolt, Anna Lou‐Hing, Karl M. Hanson, Nadia R. Ahmed, Ryan J. Buckley, Lou-Hing, Anna, Cooper, Christopher D. O., Bolt, Edward L., Cooper, Christopher D.O.   +13 more
core   +1 more source

Structural basis for uracil removal from DNA by human SMUG1. [PDF]

Nat Commun
Human single-strand-selective monofunctional uracil DNA glycosylase 1 (hSMUG1) removes uracil, 5-hydroxymethyluracil (5hmU) and 5-fluorouracil (5FU) from DNA, thereby initiating the base excision repair (BER) process.
Ludäscher JM, Scaletti Hutchinson E, Vila-Julià G, Jemth AS, Shahid S, Wiita E, Cabeza de Vaca I, Pach S, Gajdos L, Aggarwal S, Walse E, Mortusewicz O, Helleday T, Carlsson J, Stenmark P.   +14 more
europepmc   +3 more sources

A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine [PDF]

, 2014
We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme’s active ...
Fox, Keith R., Kimber, Scott T., Keith R. Fox, Brown, T, Fox, KR, Tom Brown, Brown, Tom, Brown, Thomas, Kimber, ST, Scott T. Kimber, Scott T Kimber, Keith R Fox   +11 more
core   +2 more sources

Functional Characterization of Excision Repair and RecA-Dependent Recombinational DNA Repair in Campylobacter jejuni [PDF]

, 2009
The presence and functionality of DNA repair mechanisms in Campylobacter jejuni are largely unknown. In silico analysis of the complete translated genome of C.
Dep Infectieziekten Immunologie, de Boer, A.G., Graaf-van Bloois, L., van der, Gaasbeek, E.J., Strategic Infection Biology, van Putten, J.P.M., van der Graaf-van Bloois, L., Boer, P., Wagenaar, J.A., Vermaning, B.J., van der Wal, F.J., Wal, F.J., van der, Putten, J.P.M., van, Boer, A.G., de   +13 more
core   +2 more sources

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase [PDF]

, 2013
Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis.
Cole, Ambrose R., Savva, Renos, Baltulionis, Gediminas, Ofer, S, Hornyak, P, Ryzhenkova, K, Baltulionis, G, Cole, AR, Ofer, Sapir, Savva, R, Ryzhenkova, Ksenia, Hornyak, Peter   +11 more
core   +1 more source

Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]

, 2013
The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
Stier, Ildikó, Antal Kiss (474840), Ildikó Stier, Antal Kiss, Kiss, Antal, Ildikó Stier (474839)   +5 more
core   +2 more sources

A function of thymine DNA glycosylase-initiated DNA repair in maintaining epigenome stability [PDF]

, 2013
The Thymine DNA Glycosylase (TDG) was initially discovered by its ability to excise the deamination products of cytosine and 5-methylcytosine (5-mC), and therefore thought to initiate base excision repair (BER) of the resulting G•U and G•T mismatches.
Jacobs, Angelika L.
core   +1 more source

Ground State Destabilization in Uracil DNA Deglycosylase (UDG): Let’s Not Forget “Tautomeric Strain” in Substrates [PDF]

, 2019
QM and QM/MM computations reveal that some enzymes, like the uracil DNA deglycosylase, may achieve ground state destabilization through substrate "tautomeric strain.
G. Andres, Cisneros, Judy, Wu, Ranjita, Das, Erik A., Vázquez-Montelongo   +3 more
core   +1 more source

The importance of the fifth nucleotide in DNA: Uracil

, 2023
Uracil is a ribonucleotide found in both DNA and RNA, with the main difference between the two being the presence of thymine in DNA and uracil in RNA. Although thymine and uracil are similar in function and can form the same base pairs with adenine, the ...
Roberts, Jamie Z., LaBonte , Melissa J.; id_orcid   +1 more
core   +1 more source

Targeted rapid amplification of cDNA ends (T-RACE)-an improved RACE reaction through degradation of non-target sequences [PDF]

, 2010
Amplification of the 5' ends of cDNA, although simple in theory, can often be difficult to achieve. We describe a novel method for the specific amplification of cDNA ends. An oligo-dT adapter incorporating a dUTP-containing PCR primer primes first-strand
Bower, Neil I., I. A. Johnston, N. I. Bower, Johnston, Ian A.   +3 more
core   +1 more source