Results 21 to 30 of about 1,466,903 (122)
Precluding uracil from DNA [PDF]
, 1996 Two enzymes, dUTP pyrophosphatase and uracil-DNA glycosylase, prevent the misincorporation of uracil into the genome in distinct manners. The atomic structures of these proteins complexed with substrate analogs reveal the structural basis for uracil ...
Vassylyev, Dmitry G, Morikawa, Kosuke
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Characterization of Escherichia coli double-strand uracil-DNA glycosylase and analysis of uracil-initiated base excision DNA repair [PDF]
Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized
Sung, Jung-Suk
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Plant Biotechnology Journal, Volume 23, Issue 11, Page 5146-5160, November 2025.ABSTRACT Nitrogen availability remains a principal constraint to crop productivity. Plants cannot directly assimilate the abundant nitrogen available in our atmosphere; instead, they rely on the uptake of inorganic forms of nitrogen, such as ammonium and nitrate from the soil.
Hiromi Tajima +6 more
wiley +1 more source
Uracil-density measured by the long amplicon qPCR approach.
, 2018 A) Relative percent amplification of the genomic DNA samples from the yeast cells treated with the indicated concentration of 5-FU for 24 hrs. B) The uracil-density of the genomic DNA samples calculated from the relative percent amplification shown in A).
Nayun Kim (481079) +3 more
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, 1978 Uracil is incorporated into newly synthesized DNA by mutants of E. coli with reduced levels of dUTPase (dUTP nucleotidohydrolase; EC 3.6.1.23). Excision-repair of the incorporated uracil results in the generation of labeled DNA fragments that appear ...
Warner, H. R. +4 more
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Chemistry &Biodiversity, Volume 22, Issue 9, September 2025.ABSTRACT The subfamily Gomphrenoideae is composed of about 480 accepted species, many of which have been historically used as medicinal plants, reason why they have been studied in terms of chemical profile, biological activity, and safety. This review consolidates the advances in research on this subfamily over the past 47 years, emphasizing its ...
Dayanna Isabel Araque Gelves +3 more
wiley +1 more source
Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase
, 1977 Mutants of Escherichia coli that are severely defective in the enzyme dUTPase (dut) accumulate short (4 to 5 S) Okazaki fragments following brief pulses with [3H]thymidine. The transient appearance of DNA fragments in these mutants is plausibly explained
Tye, Bik Kwoon, Lehman, I. R.
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Utilising Uracil DNA Glycosylase to detect the presence of 5-Methylcytosine
, 2014 DNA is regularly subjected to endogenous and exogenous reagents that cause mutations that can be detrimental to a cell if they are not repaired. One class of enzymes responsible for DNA repair is the family of DNA glycosylases and their role is to remove
Kimber, Scott T.
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Angewandte Chemie, Volume 137, Issue 34, August 18, 2025.Pseudouridin‐5'‐triphosphat (ΨTP) und sein N1‐methyliertes Derivat (m1ΨTP) sind entscheidende Komponenten in der mRNA‐Impfstofftechnologie. Hier wird ein atomökonomischer, ertragreicher biokatalytischer Weg zur Synthese von ΨTP vorgestellt. Zudem wurde ein neuer chemo‐enzymatischer Weg für die m1ΨTP‐Synthese entwickelt.
Martin Pfeiffer +4 more
wiley +1 more source
Uracil moieties in Plasmodium falciparum genomic DNA [PDF]
, 2018 Plasmodium falciparum parasites undergo multiple genome duplication events during their development. Within the intraerythrocytic stages, parasites encounter an oxidative environment and DNA synthesis necessarily proceeds under these circumstances.
G. Vértessy, Beáta +9 more
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