Results 21 to 30 of about 1,296 (134)

A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine

, 2014
We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme’s active ...
Fox, Keith R., Kimber, Scott T., Keith R. Fox, Brown, T, Fox, KR, Tom Brown, Brown, Tom, Brown, Thomas, Kimber, ST, Scott T. Kimber, Scott T Kimber, Keith R Fox   +11 more
core   +1 more source

The cytotoxic activity of ribosome-inactivating protein saporin-6 is attributed to its rRNA N-glycosidase and internucleosomal DNA fragmentation activities [PDF]

, 2002
Saporin-6 produced by the plant Saponaria officinalis belongs to the family of single chain ribosome-inactivating proteins. It potently inhibits protein synthesis in eukaryotic cells, by cleaving the N-glycosidic bond of a specific adenine in 28 S rRNA ...
Bagga, Shveta, Batra, Janendra K., Seth, Divya   +2 more
core   +1 more source

Functional Characterization of Excision Repair and RecA-Dependent Recombinational DNA Repair in Campylobacter jejuni

, 2009
The presence and functionality of DNA repair mechanisms in Campylobacter jejuni are largely unknown. In silico analysis of the complete translated genome of C.
Dep Infectieziekten Immunologie, de Boer, A.G., Graaf-van Bloois, L., van der, Gaasbeek, E.J., Strategic Infection Biology, van Putten, J.P.M., van der Graaf-van Bloois, L., Boer, P., Wagenaar, J.A., Vermaning, B.J., van der Wal, F.J., Wal, F.J., van der, Putten, J.P.M., van, Boer, A.G., de   +13 more
core   +1 more source

Polynucleotide: Adenosine glycosidase is the sole activity of ribosome-inactivating proteins on DNA [PDF]

, 2000
Polynucleotide: adenosine glycosidases (PNAG) are a class of plant and bacterial enzymes commonly known as ribosome-inactivating proteins (RIP). They are presently classified as rRNA N-glycosidases in the enzyme nomenclature [EC 3.2.2.22].
Luigi Barbieri, Paola Gorini, Paola Valbonesi, Giampaolo Zuccheri, Bruno Samori, Fiorenzo Stirpe, Federica Righi, Federica Monti   +7 more
core   +1 more source

Uracil incorporation: A source of pulse-labelled DNA fragments in the replication of the Escherichia coli chromosome

, 1978
Uracil is incorporated into newly synthesized DNA by mutants of E. coli with reduced levels of dUTPase (dUTP nucleotidohydrolase; EC 3.6.1.23). Excision-repair of the incorporated uracil results in the generation of labeled DNA fragments that appear ...
Warner, H. R., Chien, J., Lehman, I. R., Duncan, B. K., Tye, B. K.   +4 more
core   +1 more source

Evolutionary divergence and functional insights into the heteromeric cis‐prenyltransferase of Paramecium tetraurelia

The FEBS Journal, Volume 293, Issue 6, Page 1681-1707, March 2026.
Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk, Kamil Steczkiewicz, Mariusz Radkiewicz, Paweł Link‐Lenczowski, Przemysław Surowiecki, Karolina Sztompka, Kariona A. Grabińska, Jacek K. Nowak, Liliana Surmacz   +8 more
wiley   +1 more source

Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase

, 1977
Mutants of Escherichia coli that are severely defective in the enzyme dUTPase (dut) accumulate short (4 to 5 S) Okazaki fragments following brief pulses with [3H]thymidine. The transient appearance of DNA fragments in these mutants is plausibly explained
Tye, Bik Kwoon, Lehman, I. R.
core   +1 more source

Extensive lysine methylation in hyperthermophilic crenarchaea : potential implications for protein stability and recombinant enzymes [PDF]

, 2010
In eukarya and bacteria, lysine methylation is relatively rare and is catalysed by sequence-specific lysine methyltransferases that typically have only a single-protein target.
Botting, Catherine H., White, Malcolm F., Talbot, Paul, Malcolm F. White, Paytubi, Sonia, Paul Talbot, Catherine H. Botting, Sonia Paytubi   +7 more
core   +1 more source

Pseudouridine Monophosphate Glycosidase: A New Glycosidase Mechanism

, 2016
Pseudouridine (Ψ), the most abundant modification in RNA, is synthesized in situ using Ψ synthase. Recently, a pathway for the degradation of Ψ was described [Preumont, A., Snoussi, K., Stroobant, V., Collet, J. F., and Van Schaftingen, E. (2008) J. Biol.
Tadhg P. Begley (170128), Nilkamal Mahanta (1898020), Siyu Huang (2024458), Steven E. Ealick (43371)   +3 more
core   +1 more source

Increased Apigenin in DNA‐Edited Hexaploid Wheat Promoted Soil Bacterial Nitrogen Fixation and Improved Grain Yield Under Limiting Nitrogen Fertiliser

Plant Biotechnology Journal, Volume 23, Issue 11, Page 5146-5160, November 2025.
ABSTRACT Nitrogen availability remains a principal constraint to crop productivity. Plants cannot directly assimilate the abundant nitrogen available in our atmosphere; instead, they rely on the uptake of inorganic forms of nitrogen, such as ammonium and nitrate from the soil.
Hiromi Tajima, Akhilesh Yadav, Javier Hidalgo Castellanos, Dawei Yan, Benjamin P. Brookbank, Eiji Nambara, Eduardo Blumwald   +6 more
wiley   +1 more source