Results 71 to 80 of about 7,004 (177)

An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis [corrected]. [PDF]

PLoS Genetics, 2015
Active DNA demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases.
Yan Li, Dolores Córdoba-Cañero, Weiqiang Qian, Xiaohong Zhu, Kai Tang, Huiming Zhang, Rafael R Ariza, Teresa Roldán-Arjona, Jian-Kang Zhu   +8 more
doaj   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Ginsenoside Rd Attenuates DNA Damage by Increasing Expression of DNA Glycosylase Endonuclease VIII-like Proteins after Focal Cerebral Ischemia

Chinese Medical Journal, 2016
Background: Ginsenoside Rd (GSRd), one of the main active ingredients in traditional Chinese herbal Panax ginseng, has been found to have therapeutic effects on ischemic stroke.
Long-Xiu Yang, Xiao Zhang, Gang Zhao
doaj   +1 more source

A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily [PDF]

Nucleic Acids Research, 2003
Cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was ...
Ji Hyung, Chung, Eun Kyoung, Im, Hyun-Young, Park, Jun Hye, Kwon, Seahyoung, Lee, Jaewon, Oh, Ki-Chul, Hwang, Jong Ho, Lee, Yangsoo, Jang   +8 more
openaire   +2 more sources

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Identification of Escherichia coli Mismatch-specific Uracil DNA Glycosylase as a Robust Xanthine DNA Glycosylase [PDF]

Journal of Biological Chemistry, 2010
The gene for the mismatch-specific uracil DNA glycosylase (MUG) was identified in the Escherichia coli genome as a sequence homolog of the human thymine DNA glycosylase with activity against mismatched uracil base pairs. Examination of cell extracts led us to detect a previously unknown xanthine DNA glycosylase (XDG) activity in E. coli.
Hyun-Wook, Lee, Allyn R, Brice, Charles B, Wright, Brian N, Dominy, Weiguo, Cao   +4 more
openaire   +2 more sources

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

Mechanism of Werner DNA helicase: POT1 and RPA stimulates WRN to unwind beyond gaps in the translocating strand. [PDF]

PLoS ONE, 2009
WRN belongs to the RecQ family of DNA helicases and it plays a role in recombination, replication, telomere maintenance and long-patch base excision repair. Here, we demonstrate that WRN efficiently unwinds DNA substrates containing a 1-nucleotide gap in
Byungchan Ahn, Jae Wan Lee, Hana Jung, Gad Beck, Vilhelm A Bohr   +4 more
doaj   +1 more source

DNA Methylation Shapes Seed‐Borne Microbiome and Proteome Responses During Early Maize‐Beneficial Bacteria Interactions

Plant, Cell &Environment, EarlyView.
ABSTRACT Microorganism and plant interactions are crucial for development and environmental adaptation. Plant growth promoting bacteria enhance agricultural productivity in a sustainable manner, while epigenetic modifications such as DNA methylation regulate gene expression and adaptive responses.
Pedro Igor Zocateli, Geovanna Vitória Olímpio, Felipe Astolpho de Almeida, Letícia Oliveira da Rocha, Diego Vinicius de Sena Martins, Sara Sangi, Fábio Lopes Olivares, Elyabe Monteiro de Matos, Juliana Siqueira, Lyderson Facio Viccini, Roberta Pena da Paschoa, Vanildo Silveira, Clícia Grativol   +12 more
wiley   +1 more source

Resisting Xylella fastidiosa: xylem anatomical changes in the susceptible olive cultivar Cellina di Nardò after long‐term infection

Plant Biology, EarlyView.
After long‐term infection, the susceptible O. europaea cultivar Cellina di Nardò modified its wood anatomical traits as an adaptive response to counteract Xylella fastidiosa, exhibiting spontaneous canopy restoration. Abstract The Xylella fastidiosa subsp.
E. Sabella, D. Greco, E. Nutricati, A. Frontini, L. De Bellis, M. Vergine, A. Luvisi   +6 more
wiley   +1 more source