Nei-like 1 (NEIL1) excises 5-carboxylcytosine directly and stimulates TDG-mediated 5-formyl and 5-carboxylcytosine excision [PDF]
Scientific Reports, 2017 Thymine DNA glycosylase (TDG) and Nei-like 1 (NEIL1) have both been implicated in the base excision repair step of active DNA demethylation. The robust glycosylase activity of TDG on DNA substrates containing 5-formylcytosine (5fC) or 5-carboxylcytosine (
Anton Slyvka +2 more
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A thermophilic 8-oxoguanine DNA glycosylase from Thermococcus barophiluss Ch5 is a new member of AGOG DNA glycosylase family [PDF]
Acta Biochimica et Biophysica Sinica, 2022 8-Oxoguanine (8oxoG) in DNA is a major oxidized base that poses a severe threat to genome stability. To counteract the mutagenic effect generated by 8oxoG in DNA, cells have evolved 8oxoG DNA glycosylase (OGG) that can excise this oxidized base from DNA.
Wang Lei, Jiang Donghao, Zhang Likui
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Mismatch uracil DNA glycosylase (Mug) is maintained in the Corynebacterium pseudotuberculosis genome and exhibits affinity for uracil but not other types of damage [PDF]
Genetics and Molecular BiologyThe genome of Corynebacterium pseudotuberculosis, etiologic agent of Caseous Lymphadenitis (CLA), was sequenced to comprehend its genetics, pathogenicity, and virulence mechanisms due to its economic importance.
Bruno Carvalho Resende +9 more
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Structure of a DNA Glycosylase Bound to a Nicked T:G Mismatch-Containing DNA [PDF]
MoleculesMismatched T:G base pairs can arise during de novo replication as well as base excision repair (BER). In particular, the action of the gap-filling polymerase β (Polβ) can generate a T:G pair as well as a nick in the DNA backbone.
Hala Ouzon-Shubeita +3 more
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DNA glycosylases: in DNA repair and beyond [PDF]
Chromosoma, 2018 The base excision repair machinery protects DNA in cells from the damaging effects of oxidation, alkylation, and deamination; it is specialized to fix single-base damage in the form of small chemical modifications. Base modifications can be mutagenic and/
Jacobs, Angelika, Schär, Primo
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Structural and Dynamic Features of the Recognition of 8-oxoguanosine Paired with an 8-oxoG-clamp by Human 8-oxoguanine-DNA Glycosylase [PDF]
Current Issues in Molecular Biology8-oxoguanine (oxoG) is formed in DNA by the action of reactive oxygen species. As a highly mutagenic and the most common oxidative DNA lesion, it is an important marker of oxidative stress. Human 8-oxoguanine-DNA glycosylase (OGG1) is responsible for its
Maria V. Lukina +2 more
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Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase [PDF]
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
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The compost metagenome as a source of T4 bacteriophage pyrimidine dimer glycosylase homologues [PDF]
E3S Web of Conferences, 2021 Compost is a promising source of thermotolerant enzymes for their application in biotechnology. Homologues of bacteriophage T4 DNA glycosylase can find their application in pharmaceuticals and perfumery.
Karmanova Alexandra N. +2 more
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Open Biology, 2021 Base excision repair is one of the important DNA repair mechanisms in cells. The fundamental role in this complex process is played by DNA glycosylases.
Anna Ligasová +4 more
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Biochemistry and Biophysics Reports, 2021 Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair.
Avani Mehta +4 more
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