Results 11 to 20 of about 1,564,144 (179)
A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
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Mismatch uracil DNA glycosylase (Mug) is maintained in the Corynebacterium pseudotuberculosis genome and exhibits affinity for uracil but not other types of damage [PDF]
Genetics and Molecular BiologyThe genome of Corynebacterium pseudotuberculosis, etiologic agent of Caseous Lymphadenitis (CLA), was sequenced to comprehend its genetics, pathogenicity, and virulence mechanisms due to its economic importance.
Bruno Carvalho Resende +9 more
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Structure of a DNA Glycosylase Bound to a Nicked T:G Mismatch-Containing DNA [PDF]
MoleculesMismatched T:G base pairs can arise during de novo replication as well as base excision repair (BER). In particular, the action of the gap-filling polymerase β (Polβ) can generate a T:G pair as well as a nick in the DNA backbone.
Hala Ouzon-Shubeita +3 more
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Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex [PDF]
Communications Biology, 2021 Ahmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a ...
Arash Ahmadi +10 more
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Nature Communications, 2020 DNA glycosylases are lesion-specific enzymes that recognize specific nucleobase damages and catalyze their excision through cleavage of the glycosidic bond.
Uddhav K. Shigdel +6 more
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Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase [PDF]
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
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Nucleobase catalysts for the enzymatic activation of 8-oxoguanine DNA glycosylase 1. [PDF]
RSC Chem Biol: Bifunctional DNA glycosylases employ an active site lysine or the N-terminus to form a Schiff base with the abasic site (AP site) base excision repair (BER) intermediate. Cleaving this reversible structure is the rate-determining step in the initiation
Hank EC +31 more
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Open Biology, 2021 Base excision repair is one of the important DNA repair mechanisms in cells. The fundamental role in this complex process is played by DNA glycosylases.
Anna Ligasová +4 more
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The compost metagenome as a source of T4 bacteriophage pyrimidine dimer glycosylase homologues [PDF]
E3S Web of Conferences, 2021 Compost is a promising source of thermotolerant enzymes for their application in biotechnology. Homologues of bacteriophage T4 DNA glycosylase can find their application in pharmaceuticals and perfumery.
Karmanova Alexandra N. +2 more
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Acta Biochimica et Biophysica Sinica, 2022 8-Oxoguanine (8oxoG) in DNA is a major oxidized base that poses a severe threat to genome stability. To counteract the mutagenic effect generated by 8oxoG in DNA, cells have evolved 8oxoG DNA glycosylase (OGG) that can excise this oxidized base from DNA.
Wang Lei, Jiang Donghao, Zhang Likui
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