Results 11 to 20 of about 678 (148)

Noncatalytic Domains in DNA Glycosylases

International Journal of Molecular Sciences, 2022
Many proteins consist of two or more structural domains: separate parts that have a defined structure and function. For example, in enzymes, the catalytic activity is often localized in a core fragment, while other domains or disordered parts of the same protein participate in a number of regulatory processes.
Natalia A. Torgasheva, Evgeniia A. Diatlova, Inga R. Grin, Anton V. Endutkin, Grigory V. Mechetin, Ivan P. Vokhtantsev, Anna V. Yudkina, Dmitry O. Zharkov   +7 more
openaire   +2 more sources

DNA glycosylases: in DNA repair and beyond [PDF]

Chromosoma, 2011
The base excision repair machinery protects DNA in cells from the damaging effects of oxidation, alkylation, and deamination; it is specialized to fix single-base damage in the form of small chemical modifications. Base modifications can be mutagenic and/or cytotoxic, depending on how they interfere with the template function of the DNA during ...
Jacobs, A. L., Schar, P.
openaire   +6 more sources

Emergence of highly profibrotic and proinflammatory Lrat+Fbln2+ HSC subpopulation in alcoholic hepatitis

Hepatology, EarlyView., 2022
Lrat+ quiescent hepatic stellate cells (qHSC) give rise to Lrat+Fbln2+ activated HSC (aHSC) in alcohol‐associated hepatitis and this subpopulation is highly profibrotic, inflammatory, and immunoregulatory based on their single cell transcriptomic profile. Abstract Background and Aims Relative roles of HSCs and portal fibroblasts in alcoholic hepatitis (
Steven Balog, Reika Fujiwara, Stephanie Q. Pan, Khairat B. El‐Baradie, Hye Yeon Choi, Sonal Sinha, Qihong Yang, Kinji Asahina, Yibu Chen, Meng Li, Matthew Salomon, Stanley W.‐K. Ng, Hidekazu Tsukamoto   +12 more
wiley   +1 more source

Strandwise translocation of a DNA glycosylase on undamaged DNA [PDF]

Proceedings of the National Academy of Sciences, 2012
Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process.
Yan, Qi, Kwangho, Nam, Marie C, Spong, Anirban, Banerjee, Rou-Jia, Sung, Michael, Zhang, Martin, Karplus, Gregory L, Verdine   +7 more
openaire   +2 more sources

DNA glycosylases in the base excision repair of DNA [PDF]

Biochemical Journal, 1997
A wide range of cytotoxic and mutagenic DNA bases are removed by different DNA glycosylases, which initiate the base excision repair pathway. DNA glycosylases cleave the N-glycosylic bond between the target base and deoxyribose, thus releasing a free base and leaving an apurinic/apyrimidinic (AP) site.
H E, Krokan, R, Standal, G, Slupphaug
openaire   +2 more sources

An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis [corrected]. [PDF]

PLoS Genetics, 2015
Active DNA demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases.
Yan Li, Dolores Córdoba-Cañero, Weiqiang Qian, Xiaohong Zhu, Kai Tang, Huiming Zhang, Rafael R Ariza, Teresa Roldán-Arjona, Jian-Kang Zhu   +8 more
doaj   +1 more source

Mismatch uracil DNA glycosylase (Mug) is maintained in the Corynebacterium pseudotuberculosis genome and exhibits affinity for uracil but not other types of damage [PDF]

Genetics and Molecular Biology
The genome of Corynebacterium pseudotuberculosis, etiologic agent of Caseous Lymphadenitis (CLA), was sequenced to comprehend its genetics, pathogenicity, and virulence mechanisms due to its economic importance.
Bruno Carvalho Resende, Cássio Siqueira Souza Cassiano, Diego Lisboa Rios, Thalia Queiroz Ladeira, Vasco Ariston Carvalho Azevedo, Luciana Lara dos Santos, Lucía Valenzuela-Pérez, Gonzalo Cabrera, Carlos Renato Machado, Débora de Oliveira Lopes   +9 more
doaj   +1 more source

Oxanine DNA Glycosylase Activity from Mammalian Alkyladenine Glycosylase [PDF]

Journal of Biological Chemistry, 2004
Oxanine (Oxa) is a deaminated base lesion derived from guanine in which the N(1)-nitrogen is substituted by oxygen. This work reports the mutagenicity of oxanine as well as oxanine DNA glycosylase (ODG) activities in mammalian systems. Using human DNA polymerase beta, deoxyoxanosine triphosphate is only incorporated opposite cytosine (Cyt).
Hitchcock, TM, Dong, L, Connor, EE, Meira, LB, Samson, LD, Wyatt, MD, Cao, WG   +6 more
openaire   +4 more sources

Chicken MBD4 Regulates Immunoglobulin Diversification by Somatic Hypermutation

Frontiers in Immunology, 2019
Immunoglobulin (Ig) diversification occurs via somatic hypermutation (SHM) and class switch recombination (CSR), and is initiated by activation-induced deaminase (AID), which converts cytosine to uracil.
Ryan Costello, Jose F. Cantillo, Amy L. Kenter   +2 more
doaj   +1 more source

A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]

PLoS ONE, 2014
We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
doaj   +1 more source