Results 41 to 50 of about 1,564,144 (179)
Targeted rapid amplification of cDNA ends (T-RACE)-an improved RACE reaction through degradation of non-target sequences [PDF]
, 2010 Amplification of the 5' ends of cDNA, although simple in theory, can often be difficult to achieve. We describe a novel method for the specific amplification of cDNA ends. An oligo-dT adapter incorporating a dUTP-containing PCR primer primes first-strand
Bower, Neil I. +4 more
core +1 more source
A New Family of HEAT-Like Repeat Proteins Lacking a Critical Substrate Recognition Motif Present in Related DNA Glycosylases. [PDF]
PLoS ONE, 2015 DNA glycosylases are important repair enzymes that eliminate a diverse array of aberrant nucleobases from the genomes of all organisms. Individual bacterial species often contain multiple paralogs of a particular glycosylase, yet the molecular and ...
Elwood A Mullins +3 more
doaj +1 more source
Resistance-Guided Mining of Bacterial Genotoxins Defines a Family of DNA Glycosylases
mBio, 2022 Unique DNA repair enzymes that provide self-resistance against therapeutically important, genotoxic natural products have been discovered in bacterial biosynthetic gene clusters (BGCs).
Noah P. Bradley +4 more
doaj +1 more source
PLoS ONE, 2011 Orthohepadnavirus (mammalian hosts) and avihepadnavirus (avian hosts) constitute the family of Hepadnaviridae and differ by their capability and inability for expression of protein X, respectively. Origin and functions of X are unclear.
Formijn J van Hemert +5 more
doaj +1 more source
Frontiers in Microbiology, 2021 Since hyperthermophilic Archaea (HA) thrive in high-temperature environments, which accelerate the rates of deamination of base in DNA, their genomic stability is facing a severe challenge.
Tan Lin +6 more
doaj +1 more source
Redox Biology8-oxo-7,8-dihydroguanine (OG) is one of the most abundant oxidative lesions in the genome and is associated with genome instability. Its mutagenic potential is counteracted by a concerted action of 8-oxoguanine DNA glycosylase (OGG1) and mutY homolog DNA
Tobias Obermann +8 more
doaj +1 more source
Mitochondrial inhibition of uracil-DNA glycosylase is not mutagenic
Molecular Cancer, 2004 Background Uracil DNA glycosylase (UDG) plays a major role in repair of uracil formed due to deamination of cytosine. UDG in human cells is present in both the nucleus and mitochondrial compartments.
Singh Keshav K, Kachhap Sushant
doaj +1 more source
Molecular Oncology, EarlyView.Isocitrate dehydrogenase 1 (IDH1) mutations are highly recurrent in multiple human cancer types, including cholangiocarcinoma and glioma. IDH1 R132C is the most common IDH1 mutation in cholangiocarcinoma and likely arises from APOBEC3A‐ or APOBEC3B‐mediated deamination.
Kelly E. Butler +3 more
wiley +1 more source
FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. [PDF]
PLoS Genetics, 2016 FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair.
John Lalith Charles Richard +11 more
doaj +1 more source
Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo
Advanced Science, EarlyView.As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao +12 more
wiley +1 more source