Author: Saji Menon ### Reagents and solutions 1. Alkaline Solution I - 50 mM glucose - 25 mM Tris HCl (pH 8.0) - 10 mM EDTA (pH 8.0) - 10 mg/ml lysozyme - Alkaline Solution II - 0.2 N NaOH (2.0 ml of 10 N NaOH in 100ml double distilled water) - 1.0% SDS - Alkaline Solution III - 5 M Potassium acetate - Glacial acetic acid - Autoclaved
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