Results 1 to 10 of about 2,407,725 (327)

Altered DNA ligase activity in human disease. [PDF]

Mutagenesis, 2020
The joining of interruptions in the phosphodiester backbone of DNA is critical to maintain genome stability. These breaks, which are generated as part of normal DNA transactions, such as DNA replication, V(D)J recombination and meiotic recombination as ...
Tomkinson AE, Naila T, Khattri Bhandari S.   +2 more
europepmc   +2 more sources

Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation

Nucleic Acids Research, 2018
All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP) for DNA ligation. Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ...
Shih-Hsun Chen, Xiaochun Yu
openalex   +2 more sources

Rac GTPase activating protein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. [PDF]

Int J Biol Sci, 2021
Bian R, Dang W, Song X, Liu L, Jiang C, Yang Y, Li Y, Li L, Li X, Hu Y, Bao R, Liu Y.   +11 more
europepmc   +3 more sources

SUMO chain formation is required for response to replication arrest in S. pombe [PDF]

, 2009
SUMO is a ubiquitin-like protein that is post-translationally attached to one or more lysine residues on target proteins. Despite having only 18% sequence identity with ubiquitin, SUMO contains the conserved betabetaalphabetabetaalphabeta fold present in
A Gorg, A Pichler, Andrew Skilton, B Xhemalce, Brenda Mercer, C Hoege, CM Pickart, E Harlow, EA Andrews, Emily Outwin, F al-Khodairy, Felicity Z. Watts, FZ Watts, GR Bylebyl, Gustavo Goldman, H Sun, HD Ulrich, HL Klein, I Matic, J Bahler, J Prudden, JC Ho, JC Ho, Jenny C. Y. Ho, JR Mullen, K Tanaka, KP Bencsath, M Rechsteiner, M Shayeghi, MH Tatham, MH Tatham, O Kerscher, P Bayer, P Stelter, PL Kannouche, RJ Sims 3rd, RT Hay, RT Hay, S Vijay-Kumar, T Caspari, V Lallemand-Breitenbach, Y Takahashi   +41 more
core   +16 more sources

PARP-3 and APLF function together to accelerate nonhomologous end joining [PDF]

, 2010
PARP-3 is a member of the ADP-ribosyl transferase superfamily of unknown function. We show that PARP-3 is stimulated by DNA double-strand breaks (DSBs) in vitro and functions in the same pathway as the poly (ADP-ribose)-binding protein APLF to accelerate
Ahel, Ame, Anna E.O. Fisher, Audebert, Bekker-Jensen, Benjamin, Bernardo Reina-San-Martin, Boulton, Brown, Bryant, Bryant, Caldecott, Caldecott, Critchlow, Ding, Drouet, El-Khamisy, Farmer, Fisher, Grawunder, Guy Poirier, Hochegger, Hottiger, Iles, Isabelle Robert, Kanno, Keith W. Caldecott, Kickhoefer, Kleine, Le Page, Lehtio, Limei Ju, Lobrich, Loseva, Macrae, Mahaney, Maria C. Zuma, Mehrotra, Michele Rouleau, Morrison, Okano, Otto, Poirier, Robert, Roberts, Rogakou, Rogakou, Rouleau, Rulten, Smith, Soulas-Sprauel, Stevnsner, Stuart L. Rulten, Sugimura, Trucco, Wang, Yan, Yang, Yano, Yano   +59 more
core   +1 more source

Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase

Nucleic Acids Research, 2017
DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more ...
Marie-Theres Gansauge, Tobias Gerber, Isabelle Glocke, P. Korlević, Laurin Lippik, S. Nagel, L. Riehl, Anna Schmidt, M. Meyer   +8 more
semanticscholar   +1 more source

Impact of DNA ligase IV on the fidelity of end joining in human cells [PDF]

, 2003
A DNA ligase IV (LIG4)‐null human pre‐B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay.
Baldeyron, Celine, Jeggo, Penny, Kysela, B, Lieber, Michael R, Manolis, Kostas, Masson, Christel, Papadopoulo, Dora, Riballo, Enriqueta, Smith, Julianne   +8 more
core   +2 more sources

Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues

Nature Communications, 2019
African swine fever virus (ASFV) is contagious and can cause highly lethal disease in pigs. ASFV DNA ligase (AsfvLIG) is one of the most error-prone ligases identified to date; it catalyzes DNA joining reaction during DNA repair process of ASFV and plays
Yiqing Chen, Hehua Liu, Chun Yang, Yanqing Gao, Xiang Yu, Xi Chen, Ruixue Cui, Lina Zheng, Suhua Li, Xuhang Li, Jinbiao Ma, Zhen Huang, Jixi Li, J. Gan   +13 more
semanticscholar   +1 more source

Evaluation of NAD(+)-dependent DNA ligase of mycobacteria as a potential target for antibiotics [PDF]

, 2007
Mycobacteria contain genes for several DNA ligases, including ligA, which encodes a NAD+-dependent enzyme that has been postulated to be a target for novel antibacterial compounds.
Bowater, Richard P., Brzostek, Anna, Dziadek, Jarosław, Korycka-Machala, Malgorzata, Rumijowska-Galewicz, Anna, Rychta, Ewelina, Sayer, Heather R.   +6 more
core   +2 more sources

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.

ACS Synthetic Biology, 2018
Synthetic biology relies on the manufacture of large and complex DNA constructs from libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA assembly methods enable rapid construction of genes and operons through one ...
V. Potapov, J. L. Ong, R. Kucera, B. Langhorst, Katharina Bilotti, J. Pryor, E. Cantor, B. Canton, Thomas F. Knight, T. C. Evans, G. Lohman   +10 more
semanticscholar   +1 more source