Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes [PDF]
Nature Communications, 2023 Bdelloid rotifers are part of the restricted circle of multicellular animals that can withstand a wide range of genotoxic stresses at any stage of their life cycle. In this study, bdelloid rotifer Adineta vaga is used as a model to decipher the molecular
Emilien Nicolas +10 more
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The DNA binding domain and the C-terminal region of DNA Ligase IV specify its role in V(D)J recombination. [PDF]
PLoS ONE, 2023 DNA Ligase IV is responsible for the repair of DNA double-strand breaks (DSB), including DSBs that are generated during V(D)J recombination. Like other DNA ligases, Ligase IV contains a catalytic core with three subdomains-the DNA binding (DBD), the ...
Vidyasagar Malashetty +6 more
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Altered DNA ligase activity in human disease. [PDF]
Mutagenesis, 2020 The joining of interruptions in the phosphodiester backbone of DNA is critical to maintain genome stability. These breaks, which are generated as part of normal DNA transactions, such as DNA replication, V(D)J recombination and meiotic recombination as ...
Tomkinson AE +2 more
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XRCC1 Polypeptide Interacts with DNA Polymerase and Possibly Poly (ADP-Ribose) Polymerase, and DNA Ligase III Is a Novel Molecular 'Nick-Sensor' In Vitro [PDF]
Nucleic Acids Research, 1996 The DNA repair proteins XRCC1 and DNA ligase III are physically associated in human cells and directly interact in vitro and in vivo. Here, we demonstrate that XRCC1 is additionally associated with DNA polymerase-beta in human cells and that these ...
Keith W. Caldecott +3 more
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PLoS ONE, 2013 Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated.
Aya Kurosawa +6 more
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Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. [PDF]
J Clin Invest, 2018 We report the molecular, cellular, and clinical features of 5 patients from 3 kindreds with biallelic mutations in the autosomal LIG1 gene encoding DNA ligase 1.
Maffucci P +18 more
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The Sole DNA Ligase in Entamoeba histolytica Is a High-Fidelity DNA Ligase Involved in DNA Damage Repair [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 The protozoan parasite Entamoeba histolytica is exposed to reactive oxygen and nitric oxide species that have the potential to damage its genome. E. histolytica harbors enzymes involved in DNA repair pathways like Base and Nucleotide Excision Repair. The
Elisa Azuara-Liceaga +14 more
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The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis [PDF]
Microbial Cell Factories, 2019 Background Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate.
Junshu Wang +7 more
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The decrease in Rad51 and DNA ligase IV nuclear protein expression in Msh2 knockdown HC11 cells induced the low CRISPR/Cas9-mediated knock-in efficiency at the β-casein gene locus [PDF]
Animal BioscienceObjective Successful gene editing technology is crucial in molecular biology and related fields. An essential part of an efficient knock-in system is increasing homologous recombination (HR) efficiency in the double-strand break (DSB) repair pathways ...
Ga-Yeon Kim, Man-Jong Kang
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A plant DNA ligase is an important determinant of seed longevity
Plant Journal, 2010 DNA repair is important for maintaining genome integrity. In plants, DNA damage accumulated in the embryo of seeds is repaired early in imbibition, and is important for germination performance and seed longevity. An essential step in most repair pathways
Christopher E West
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