Results 1 to 10 of about 2,416,649 (374)
Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes [PDF]
Nature Communications, 2023 Bdelloid rotifers are part of the restricted circle of multicellular animals that can withstand a wide range of genotoxic stresses at any stage of their life cycle. In this study, bdelloid rotifer Adineta vaga is used as a model to decipher the molecular
Emilien Nicolas +10 more
doaj +2 more sources
The DNA binding domain and the C-terminal region of DNA Ligase IV specify its role in V(D)J recombination. [PDF]
PLoS ONE, 2023 DNA Ligase IV is responsible for the repair of DNA double-strand breaks (DSB), including DSBs that are generated during V(D)J recombination. Like other DNA ligases, Ligase IV contains a catalytic core with three subdomains-the DNA binding (DBD), the ...
Vidyasagar Malashetty +6 more
doaj +2 more sources
Altered DNA ligase activity in human disease. [PDF]
Mutagenesis, 2020 The joining of interruptions in the phosphodiester backbone of DNA is critical to maintain genome stability. These breaks, which are generated as part of normal DNA transactions, such as DNA replication, V(D)J recombination and meiotic recombination as ...
Tomkinson AE +2 more
europepmc +2 more sources
DNA ligase I, the replicative DNA ligase. [PDF]
Sub-cellular biochemistry, 2012 Multiple DNA ligation events are required to join the Okazaki fragments generated during lagging strand DNA synthesis. In eukaryotes, this is primarily carried out by members of the DNA ligase I family. The C-terminal catalytic region of these enzymes is composed of three domains: a DNA binding domain, an adenylation domain and an OB-fold domain.
Timothy R. Howes, A. Tomkinson
semanticscholar +3 more sources
Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation
Nucleic Acids Research, 2018 All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP) for DNA ligation. Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ...
Shih-Hsun Chen, Xiaochun Yu
semanticscholar +2 more sources
PLoS ONE, 2013 Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated.
Aya Kurosawa +6 more
doaj +2 more sources
The Sole DNA Ligase in Entamoeba histolytica Is a High-Fidelity DNA Ligase Involved in DNA Damage Repair [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 The protozoan parasite Entamoeba histolytica is exposed to reactive oxygen and nitric oxide species that have the potential to damage its genome. E. histolytica harbors enzymes involved in DNA repair pathways like Base and Nucleotide Excision Repair. The
Elisa Azuara-Liceaga +14 more
doaj +2 more sources
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis [PDF]
Microbial Cell Factories, 2019 Background Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate.
Junshu Wang +7 more
doaj +2 more sources
European Journal of Biochemistry, 1992 A novel form of rat liver DNA ligase (molecular mass 100 kDa) can be differentiated from DNA ligase I by several biochemical parameters. It is a more heat‐labile enzyme and unable to join bluntended DNA, even in the presence of poly(ethylene glycol) concentrations which stimulate such joining by DNA ligase I and T4 DNA ligase.
R H Elder +3 more
openaire +5 more sources
The decrease in Rad51 and DNA ligase IV nuclear protein expression in Msh2 knockdown HC11 cells induced the low CRISPR/Cas9-mediated knock-in efficiency at the β-casein gene locus [PDF]
Animal BioscienceObjective Successful gene editing technology is crucial in molecular biology and related fields. An essential part of an efficient knock-in system is increasing homologous recombination (HR) efficiency in the double-strand break (DSB) repair pathways ...
Ga-Yeon Kim, Man-Jong Kang
doaj +2 more sources