Results 21 to 30 of about 208,982 (285)

A case report of an adolescent with ligase-4 deficiency and the potential dangers of ionizing radiation in this rare patient population

Radiology Case Reports, 2021
DNA ligase IV deficiency is a rare disorder characterized by mutations in the LIG4 gene. Mutations in this gene cause a wide array of phenotypes, many of which are fatal early in life. We present an adolescent patient with heterozygous LIG4 mutations and
Priyal Chadha, BA, Ryan Thibodeau, MD, MPH, Abtin Jafroodifar, MD, Anand Majmudar, MD   +3 more
doaj   +1 more source

DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks

BMC Plant Biology, 2009
Background DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences ...
Bray Clifford M, Provost Claire M, Kozak Jaroslav, Waterworth Wanda M, Angelis Karel J, West Christopher E   +5 more
doaj   +1 more source

Mammalian DNA ligases. Catalytic domain and size of DNA ligase I. [PDF]

Journal of Biological Chemistry, 1990
DNA ligase I is the major DNA ligase activity in proliferating mammalian cells. The protein has been purified to apparent homogeneity from calf thymus. It has a monomeric structure and a blocked N-terminal residue. DNA ligase I is a 125-kDa polypeptide as estimated by sodium dodecyl sulfate-gel electrophoresis and by gel chromatography under denaturing
Dana D. Lasko, Tomas Lindahl, Alan E. Tomkinson, Graham Daly   +3 more
openaire   +2 more sources

Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain. [PDF]

PLoS ONE, 2012
To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases.
Feng Gao, Huafu Zhou, Wei Li, Xuerong Zhang   +3 more
doaj   +1 more source

A family of DNA repair ligases in bacteria? [PDF]

FEBS Letters, 2001
Geoffrey R. Weller, Aidan J. Doherty
openalex   +5 more sources

Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase

Scientific Reports, 2021
DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan, Kjersti Lian, Aili Sarre, Hanna-Kirsti S. Leiros, Adele Williamson   +4 more
doaj   +1 more source

The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining

Synthetic and Systems Biotechnology, 2019
DNA double-strand breaks (DSBs) are one of the most lethal forms of DNA damage that is not efficiently repaired in prokaryotes. Certain microorganisms can handle chromosomal DSBs using the error-prone non-homologous end joining (NHEJ) system and ...
Tianyuan Su, Fapeng Liu, Yizhao Chang, Qi Guo, Junshu Wang, Qian Wang, Qingsheng Qi   +6 more
doaj   +1 more source

Rat liver DNA ligases [PDF]

European Journal of Biochemistry, 1992
A novel form of rat liver DNA ligase (molecular mass 100 kDa) can be differentiated from DNA ligase I by several biochemical parameters. It is a more heat‐labile enzyme and unable to join bluntended DNA, even in the presence of poly(ethylene glycol) concentrations which stimulate such joining by DNA ligase I and T4 DNA ligase.
R H Elder, A Montecucco, G Ciarrocchi, J M Rossignol   +3 more
openaire   +4 more sources

DNA ligase IV and artemis act cooperatively to suppress homologous recombination in human cells: implications for DNA double-strand break repair.

PLoS ONE, 2013
Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated.
Aya Kurosawa, Shinta Saito, Sairei So, Mitsumasa Hashimoto, Kuniyoshi Iwabuchi, Haruka Watabe, Noritaka Adachi   +6 more
doaj   +1 more source

The Action of DNA Ligase at Abasic Sites in DNA [PDF]

Journal of Biological Chemistry, 1998
Apurinic/apyrimidinic (AP) sites occur frequently in DNA as a result of spontaneous base loss or following removal of a damaged base by a DNA glycosylase. The action of many AP endonuclease enzymes at abasic sites in DNA leaves a 5'-deoxyribose phosphate (dRP) residue that must be removed during the base excision repair process.
Daniel F. Bogenhagen, Kevin G. Pinz
openaire   +2 more sources