Results 21 to 30 of about 2,162,763 (370)
PLoS Genetics, 2023 The tumor suppressor BRCA1-BARD1 complex regulates many cellular processes; of critical importance to its tumor suppressor function is its role in genome integrity.
Qianyan Li +4 more
doaj +1 more source
Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes
Nature Communications, 2023 Bdelloid rotifers are part of the restricted circle of multicellular animals that can withstand a wide range of genotoxic stresses at any stage of their life cycle. In this study, bdelloid rotifer Adineta vaga is used as a model to decipher the molecular
Emilien Nicolas +10 more
doaj +1 more source
Thermostable DNA ligases from hyperthermophiles in biotechnology
Frontiers in Microbiology, 2023 DNA ligase is an important enzyme ubiquitous in all three kingdoms of life that can ligate DNA strands, thus playing essential roles in DNA replication, repair and recombination in vivo.
Jingru Shi +3 more
doaj +1 more source
Tau affects P53 function and cell fate during the DNA damage response [PDF]
Communications Biology 3, 245 (2020), 2020 Cells are constantly exposed to DNA damaging insults. To protect the organism, cells developed a complex molecular response coordinated by P53, the master regulator of DNA repair, cell division and cell fate. DNA damage accumulation and abnormal cell fate decision may represent a pathomechanism shared by aging-associated disorders such as cancer and ...
arxiv +1 more source
Identification of the DNA repair defects in a case of Dubowitz syndrome. [PDF]
PLoS ONE, 2013 Dubowitz Syndrome is an autosomal recessive disorder with a unique set of clinical features including microcephaly and susceptibility to tumor formation. Although more than 140 cases of Dubowitz syndrome have been reported since 1965, the genetic defects
Jingyin Yue +6 more
doaj +1 more source
Impact of DNA ligase IV on the fidelity of end joining in human cells [PDF]
, 2003 A DNA ligase IV (LIG4)‐null human pre‐B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay.
Baldeyron, Celine +8 more
core +2 more sources
Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase
Nucleic Acids Research, 2017 DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more ...
Marie-Theres Gansauge +8 more
semanticscholar +1 more source
Runaway Transition in Irreversible Polymer Condensation with Cyclisation [PDF]
, 2022 The process of polymer condensation, i.e. the formation of bonds between reactive end-groups, is ubiquitous in both industry and biology. Here we study generic systems undergoing polymer condensation in competition with cyclisation. Using a generalised Smoluchowski theory, molecular dynamics simulations and experiments using DNA and T4 ligase, we find ...
arxiv +1 more source
ACS Synthetic Biology, 2018 Synthetic biology relies on the manufacture of large and complex DNA constructs from libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA assembly methods enable rapid construction of genes and operons through one ...
V. Potapov +10 more
semanticscholar +1 more source
Label-free electrochemical monitoring of DNA ligase activity [PDF]
, 2008 This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I. +29 more
core +1 more source