Results 31 to 40 of about 2,416,649 (374)

Impact of DNA ligase IV on the fidelity of end joining in human cells [PDF]

, 2003
A DNA ligase IV (LIG4)‐null human pre‐B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay.
Baldeyron, Celine, Jeggo, Penny, Kysela, B, Lieber, Michael R, Manolis, Kostas, Masson, Christel, Papadopoulo, Dora, Riballo, Enriqueta, Smith, Julianne   +8 more
core   +2 more sources

A case report of an adolescent with ligase-4 deficiency and the potential dangers of ionizing radiation in this rare patient population

Radiology Case Reports, 2021
DNA ligase IV deficiency is a rare disorder characterized by mutations in the LIG4 gene. Mutations in this gene cause a wide array of phenotypes, many of which are fatal early in life. We present an adolescent patient with heterozygous LIG4 mutations and
Priyal Chadha, BA, Ryan Thibodeau, MD, MPH, Abtin Jafroodifar, MD, Anand Majmudar, MD   +3 more
doaj   +1 more source

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.

ACS Synthetic Biology, 2018
Synthetic biology relies on the manufacture of large and complex DNA constructs from libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA assembly methods enable rapid construction of genes and operons through one ...
V. Potapov, J. L. Ong, R. Kucera, B. Langhorst, Katharina Bilotti, J. Pryor, E. Cantor, B. Canton, Thomas F. Knight, T. C. Evans, G. Lohman   +10 more
semanticscholar   +1 more source

Evaluation of NAD(+)-dependent DNA ligase of mycobacteria as a potential target for antibiotics [PDF]

, 2007
Mycobacteria contain genes for several DNA ligases, including ligA, which encodes a NAD+-dependent enzyme that has been postulated to be a target for novel antibacterial compounds.
Bowater, Richard P., Brzostek, Anna, Dziadek, Jarosław, Korycka-Machala, Malgorzata, Rumijowska-Galewicz, Anna, Rychta, Ewelina, Sayer, Heather R.   +6 more
core   +2 more sources

DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks

BMC Plant Biology, 2009
Background DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences ...
Bray Clifford M, Provost Claire M, Kozak Jaroslav, Waterworth Wanda M, Angelis Karel J, West Christopher E   +5 more
doaj   +1 more source

T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction

Nucleic Acids Research, 2018
DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be discovered and is widely used in molecular biology, but its structure remained unknown. Our crystal structure of T4
K. Shi, T. Bohl, Jeonghyun Park, A. Zasada, Shray Malik, Surajit Banerjee, V. Tran, Na Li, Zhiqi Yin, F. Kurniawan, K. Orellana, H. Aihara   +11 more
semanticscholar   +1 more source

DNA ligase IV syndrome; a review

Orphanet Journal of Rare Diseases, 2016
DNA ligase IV deficiency is a rare primary immunodeficiency, LIG4 syndrome, often associated with other systemic features. DNA ligase IV is part of the non-homologous end joining mechanism, required to repair DNA double stranded breaks.
T. Altmann, A. Gennery
semanticscholar   +1 more source

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
core   +1 more source

Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK. [PDF]

, 2009
Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand.
Ali, Ammar A. E., Jukes, Rachel M., Oliver, Antony W., Pearl, Laurence H.   +3 more
core   +3 more sources

Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain. [PDF]

PLoS ONE, 2012
To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases.
Feng Gao, Huafu Zhou, Wei Li, Xuerong Zhang   +3 more
doaj   +1 more source