A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation [PDF]
BMC Microbiology, 2023 Background The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species.
Jolyn Pan +4 more
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Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase [PDF]
Scientific Reports, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan +4 more
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Crystal structure of ATP-dependent DNA ligase from Rhizobium phage vB_RleM_P10VF. [PDF]
Acta Crystallogr F Struct Biol CommunWe have determined the structure of the Rhizobium phage vB_RleM_P10VF DNA ligase bound to a nicked DNA duplex to 2.2 Å resolution. The DNA ligase exhibits a canonical DNA ligase-binding mode fully encircling the duplex and has considerable structural ...
Rothweiler U, Leiros HKS, Williamson A.
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T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction [PDF]
Nucleic Acids Research, 2018 DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be discovered and is widely used in molecular biology, but its structure remained unknown. Our crystal structure of T4
Ke Shi +11 more
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Enzyme–adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface [PDF]
Acta Crystallographica Section D Biological Crystallography, 2014 The enzyme–adenylate structure of a bacterial ATP-dependent DNA ligase (ADL), which does not have any additional DNA-binding domains, is similar to minimal viral ADLs that comprise only the core catalytic domains.
Adele Williamson +2 more
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A Primer-dependent Polymerase Function of Pseudomonas aeruginosa ATP-dependent DNA Ligase (LigD) [PDF]
Journal of Biological Chemistry, 2004 Pseudomonas aeruginosa encodes two putative DNA ligases: a classical NAD+-dependent DNA ligase (LigA) plus an ATP-dependent DNA ligase (LigD). LigD exemplifies a family of bacterial proteins that consist of a ligase domain fused to flanking domains that ...
Hui Zhu, Stewart Shuman
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Structures of ATP-bound DNA ligase D in a closed domain conformation reveal a network of amino acid and metal contacts to the ATP phosphates [PDF]
Journal of Biological Chemistry, 2019 DNA ligases are the sine qua non of genome integrity and essential for DNA replication and repair in all organisms. DNA ligases join 3′-OH and 5′-PO4 ends via a series of three nucleotidyl transfer steps. In step 1, ligase reacts with ATP or NAD+ to form
Mihaela-Carmen Unciuleac +2 more
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BiofilmNeisseria gonorrhoeae, the causative agent of the sexually transmitted infection, gonorrhoea, is known to form biofilms rich in extracellular DNA on human cervical cells. Biofilm formation is conducive to increased antimicrobial resistance and evasion of
Jolyn Pan +4 more
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Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
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Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification [PDF]
Nature CommunicationsBacteriophages possess a wide array of DNA modifications, with many acting as molecular camouflage to evade host immune defenses. Sequence databases contain numerous bacteriophage enzymes of unknown function, with some potentially involved in yet to be ...
Qiaoyu Yang +16 more
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