Results 291 to 300 of about 182,062 (331)

ATP-type DNA ligase requires other proteins for its activity in vitro and its operon components for radiation resistance in Deinococcus radiodurans in vivo

Biochemistry and Cell Biology, 2010
A multiprotein DNA processing complex isolated from Deinococcus radiodurans contains the DNA repair protein PprA, an ATP-type DNA repair ligase (LigB) encoded by the drB0100 gene, and protein kinase activity. An ATP-dependent DNA end-joining activity was detected in the complex.
Yogendra S. Rajpurohit, Swathi Kota, Hari S. Misra, Vidya A. Kamble   +3 more
openaire   +4 more sources

A sensitive ligase-based ATP electrochemical assay using molecular beacon-like DNA

Biosensors and Bioelectronics, 2010
A sensitive and selective ligase-based signal-on electrochemical sensing method for adenosine-5'-triphosphate (ATP) detection had been developed using molecular beacon (MB)-like DNA. In this method, the biotin-tagged MB-like DNA was self-assembled onto a gold electrode to form a stem-loop structure by means of facile gold-thiol chemistry, which ...
Xiaoqi Ni, Kemin Wang, Yonghong Wang, Xiaoxiao He   +3 more
openaire   +4 more sources

Interaction of β-L-Adenosine-5′-triphosphate (L-ATP) with Human Deoxycytidine Kinase, Human DNA Primase and T4 DNA Ligase: Does the Chance Direct Enzymatic Enantioselectivity?

Nucleosides and Nucleotides, 1999
We demonstrate that L-ATP: 1) as well as its natural D-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human DNA-primase and the ATP-dependent T4 DNA ligase. Thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would ...
A. Montecucco, Gilles Gosselin, Federico Focher, Annalisa Verri, Jean Louis Imbach, Silvio Spadari, V. Boudou   +6 more
openaire   +4 more sources

Functional domains of an ATP-dependent DNA ligase.

Journal of molecular biology, 1999
The crystal structure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two structural domains. In order to investigate the biochemical activities of these domains, we have overexpressed them separately and purified them to homogeneity. The larger N-terminal domain retains adenylation and ligase activities, though
A J, Doherty, D B, Wigley
openaire   +3 more sources

Functional domains of an ATP-dependent DNA ligase 1 1Edited by A. R. Fersht

Journal of Molecular Biology, 1999
Aidan J. Doherty, Dale B. Wigley
openaire   +3 more sources

DNA Ligase From Thermococcus radiotolerans: Recombinant Production, Characterization, Up-Scale Fermentation, and In Silico Evaluation.

Biotechnology and applied biochemistry
The present study describes molecular cloning, Escherichia coli expression, purification, and characterization of a DNA ligase from Thermococcus radiotolerans (TR). Optimal pH, temperature, media composition, and inducer concentrations for the production
Ahmed Osman, S. Iftikhar, B. N. Murtaza, Salman Hosawi, Hisham N Tayeb, Thamir M. Eid, Hayam A Alwabsi, Muhammad Shahid Nadeem   +7 more
semanticscholar   +1 more source

Synthesis and bioevaluation of aryl hydroxamates distinguishing between NAD+ and ATP-dependent DNA ligases

MedChemComm, 2012
Identification of compounds distinguishing between NAD+ and ATP-dependent ligases is a key factor to discover new antimicrobials. Based on virtual screening experiments a series of hydroxamates distinguishing between the above two classes of enzymes are synthesized.
Vandna Kukshal, Arya Ajay, Rahul Sharma, Divya Dube, Taran Khanam, Deepti Chopra, Ravishankar Ramachandran, Rama P. Tripathi, Mridul Mishra   +8 more
openaire   +2 more sources

A new group of plant-specific ATP-dependent DNA ligases identified by protein phylogeny, hydrophobic cluster analysis and 3-dimensional modelling

Functional Plant Biology, 2005
The eukaryotic ATP-dependent DNA ligases comprise a group of orthologous proteins that have distinct roles in DNA metabolism. In contrast with the well-known DNA ligases of animal cells, the DNA ligases of plant cells are poorly described. Until now, only two DNA ligases (I and IV) genes of Arabidopsis thaliana (L.) Heynh were isolated and ...
Martin Brendel, Martin Brendel, Diego Bonatto, João Antonio Pêgas Henriques, João Antonio Pêgas Henriques   +4 more
openaire   +3 more sources

Rapid Time Scale Analysis of T4 DNA Ligase-DNA Binding.

Biochemistry, 2017
DNA ligases, essential to both in vivo genome integrity and in vitro molecular biology, catalyze phosphodiester bond formation between adjacent 3'-OH and 5'-phosphorylated termini in dsDNA.
R. Bauer, Thomas J. Jurkiw, T. C. Evans, G. Lohman   +3 more
semanticscholar   +1 more source

ATP-DEPENDENT DNA LIGASE FROM PYROCOCCUS FURIOSUS

, 2006
H Nishida, Yoshizumi Ishino, Kosuke Morikawa   +2 more
openalex   +2 more sources