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DNA ligase-AMP adducts: Identification of yeast DNA ligase polypeptides
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1985Yeast DNA ligase is radioactively labelled in vitro by incubating a crude cell extract with [alpha-32P]ATP. The product of this reaction is the stable covalent ligase-AMP adduct, which can be characterized by its reactivity with either pyrophosphate or nicked DNA and visualized by gel electrophoresis and autoradiography.
Geoffrey R. Banks, David G. Barker
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Ligation of DNA with T4 DNA Ligase
2003Since they are involved in such important processes as DNA replication, DNA repair, and DNA recombination, DNA ligases can be found in all living cells. Two prokaryotic DNA ligases have become indispensible tools in the fields of in vitro DNA recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and
Wim Gaastra, Kirsten Hansen
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Leukemia Research, 1985
Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
Jean-Pierre Marie +9 more
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Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
Jean-Pierre Marie +9 more
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DNA Polymerases and DNA Ligases
2013DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA ...
Sonoko Ishino, Yoshizumi Ishino
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Prokaryotic DNA Ligases Unwind Superhelical DNA
Biochemical and Biophysical Research Communications, 1996We have studied the effect on DNA topology of binding of prokaryotic DNA ligases (T4 and E. coli) to superhelical or nicked circular DNA. Performing topoisomerase I-mediated relaxation in the presence of increasing amounts of T4 ligase led to a shift in the topoisomer distribution to increasingly more negative values.
Kensal E. van Holde +3 more
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DNA ligases and ligase-based technologies
Clinical and Applied Immunology Reviews, 2001Abstract DNA ligases catalyze the strand joining reaction at a nick junction. The requirement of base-pair complementarity at the nick junction has been explored for development of ligase-based technologies for mutation detection. In oligonucleotide ligation assay (OLA), two DNA probes complementary to the target sequence are joined by DNA ligase ...
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1982
Publisher Summary DNA ligases refer to enzymes that catalyze the formation of a phosphodiester linkage between DNA chains. DNA ligases are essential reagents in studies on nucleic acid structure and metabolism. Their value derives from the specificity of the reaction and their ability to join polynucleotide chains covalently.
Charles C. Richardson, M J Engler
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Publisher Summary DNA ligases refer to enzymes that catalyze the formation of a phosphodiester linkage between DNA chains. DNA ligases are essential reagents in studies on nucleic acid structure and metabolism. Their value derives from the specificity of the reaction and their ability to join polynucleotide chains covalently.
Charles C. Richardson, M J Engler
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DNA ligase is required for encapsidation of bacteriophage T4 DNA
Journal of Molecular Biology, 1981Abstract Phage T4 DNA ligase, in addition to its early role in DNA metabolism, is also required later in T4 development, following the assembly and cleavage of the T4 prohead (Hsiao & Black, 1977). A thin-section electron microscope study revealed that the late ligase function is required for completion, but probably not initiation, of T4 DNA ...
L.W. Black, A. Zachary
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DNA Ligase: Structure, Mechanism, and Function
Science, 1974DNA ligase of E. coli is a single polypeptide of molecular weight 75,000. The comparable T4-induced enzyme is somewhat smaller (63,000 to 68,000). Both enzymes catalyze the synthesis of phosphodiester bonds between adjacent 5′-phosphoryl and 3′-hydroxyl groups in nicked duplex DNA, coupled to the cleavage of the ...
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Eukaryotic DNA Ligases and DNA Repair
1998The correct joining of appropriate DNA ends is a fundamental step in many important reactions within the cell and a number of enzymes have been identified which perform these end-joining reactions. Some enzymes execute a ligation step as an intermediate of their overall reaction mechanism; DNA topoisomerases are a prime example of this type of activity
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