Results 11 to 20 of about 2,144,804 (323)
DNA ligases: progress and prospects. [PDF]
J Biol Chem, 2009 DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates.
Shuman S.
europepmc +2 more sources
Inactivating UBE2M impacts the DNA damage response and genome integrity involving multiple cullin ligases. [PDF]
PLoS ONE, 2014 Protein neddylation is involved in a wide variety of cellular processes. Here we show that the DNA damage response is perturbed in cells inactivated with an E2 Nedd8 conjugating enzyme UBE2M, measured by RAD51 foci formation kinetics and cell based DNA ...
Scott Cukras +3 more
doaj +3 more sources
Biochemical and Genetic Analysis of the Four DNA Ligases of Mycobacteria [PDF]
Journal of Biological Chemistry, 2004 Mycobacterium tuberculosis encodes an NAD+-dependent DNA ligase (LigA) plus three distinct ATP-dependent ligase homologs (LigB, LigC, and LigD). Here we purify and characterize the multiple DNA ligase enzymes of mycobacteria and probe genetically whether
Chunling Gong +4 more
openalex +2 more sources
DNA ligases as therapeutic targets. [PDF]
Transl Cancer Res, 2013 Tomkinson AE, Howes TR, Wiest NE.
europepmc +2 more sources
SUMO chain formation is required for response to replication arrest in S. pombe [PDF]
, 2009 SUMO is a ubiquitin-like protein that is post-translationally attached to one or more lysine residues on target proteins. Despite having only 18% sequence identity with ubiquitin, SUMO contains the conserved betabetaalphabetabetaalphabeta fold present in
A Gorg +41 more
core +19 more sources
Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
doaj +1 more source
Plant SUMO E3 Ligases: Function, Structural Organization, and Connection With DNA
Frontiers in Plant Science, 2021 Protein modification by the small ubiquitin-like modifier (SUMO) plays an important role in multiple plant processes, including growth, development, and the response to abiotic stresses.
Souleimen Jmii, Laurent Cappadocia
doaj +1 more source
Ubiquitylation in immune disorders and cancer: from molecular mechanisms to therapeutic implications [PDF]
, 2012 Conjugation of ubiquitin to proteins (ubiquitylation) has emerged to be one of the most crucial post-translational modifications controlling virtually all cellular processes.
Fulda, Simone +2 more
core +2 more sources
Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain. [PDF]
PLoS ONE, 2012 To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases.
Feng Gao +3 more
doaj +1 more source
Biomedicines, 2023 Ubiquitination is a post-translational modification (PTM) that is involved in proteolysis, protein–protein interaction, and signal transduction. Accumulation of mutations and genomic instability are characteristic of cancer cells, and dysfunction of the ...
Hae Ryung Chang
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