Results 101 to 110 of about 2,560,073 (378)
Development and application of a microarray meter tool to optimize microarray experiments
BMC Research Notes, 2008 Background Successful microarray experimentation requires a complex interplay between the slide chemistry, the printing pins, the nucleic acid probes and targets, and the hybridization milieu.
Rouse Richard JD +8 more
doaj +1 more source
Molecular Oncology, EarlyView.This study indicates that Merkel cell carcinoma (MCC) does not originate from Merkel cells, and identifies gene, protein & cellular expression of immune‐linked and neuroendocrine markers in primary and metastatic Merkel cell carcinoma (MCC) tumor samples, linked to Merkel cell polyomavirus (MCPyV) status, with enrichment of B‐cell and other immune cell
Richie Jeremian +10 more
wiley +1 more source
Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events [PDF]
, 2018 The clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout.
Sevenler, Derin +2 more
core +2 more sources
Optimization of Oligonucleotide DNA Microarrays
, 2007 Optimization of oligonucleotide DNA microarrays is a multiparametric problem. The goal of the optimization process is to get conditions that capture target DNA with high sensitivity and selectivity. Parameters determining the performance of the microarray are spot morphology, probe and target density, background, and selectivity. More than 10 variables
Martin Dufva, Claus Christensen
openaire +3 more sources
DNA Microarray Technology [PDF]
, 2002 Collaboration between Sandia National Laboratories and the University of New Mexico Biology Department resulted in the capability to train students in microarray techniques and the interpretation of data from microarray experiments. These studies provide for a better understanding of the role of stationary phase and the gene regulation involved in exit
WERNER-WASHBURNE, MARGARET +1 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli +4 more
wiley +1 more source
Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array
BMC Genomics, 2011 Background Recent progress in high-throughput technologies has greatly contributed to the development of DNA methylation profiling. Although there are several reports that describe methylome detection of whole genome bisulfite sequencing, the high cost ...
Zhao Qian +6 more
doaj +1 more source
Advanced Functional Materials, EarlyView.A miniaturized, label‐free, and enzyme‐free biosensor (miR‐TFT) enables direct electrical detection of microRNA (miRNA) with single‐nucleotide specificity and a detection limit of 0.6 fM. Built on a tri‐channel In2O3/ZnO heterojunction and functionalized with bespoke peptide nucleic acid (PNA) probes, the device is robust, reusable, and compatible with
Wejdan S. Al Ghamdi +5 more
wiley +1 more source
An Overview of DNA Microarray Grid Alignment and Foreground Separation Approaches
EURASIP Journal on Advances in Signal Processing, 2006 This paper overviews DNA microarray grid alignment and foreground separation approaches. Microarray grid alignment and foreground separation are the basic processing steps of DNA microarray images that affect the quality of gene expression information ...
Bajcsy Peter
doaj +1 more source
BMC Genomics, 2003 Background The ADGE technique is a method designed to magnify the ratios of gene expression before detection. It improves the detection sensitivity to small change of gene expression and requires small amount of starting material. However, the throughput
Ile Kristina E +4 more
doaj +1 more source