Results 101 to 110 of about 2,576,927 (378)

Multi‐omic profiling of squamous cell lung cancer identifies metabolites and related genes associated with squamous cell carcinoma

Molecular Oncology, EarlyView.
Using multi‐omic characterization, we aimed to identify key regulators specific to squamous cell lung carcinoma (SqCC). SqCC‐specific differentially expressed genes were integrated with metabolics data. High expression of the creatine transporter SLC6A8, along with elevated creatine levels, appeared to be a distinct metabolic feature of SqCC.
Johan Staaf, Daniel Ehinger, Hans Brunnström, Mats Jönsson, Frida Rosengren, Marija Kotevska, Anna Karlsson, Mattias Aine, Christian Frezza, Maria Planck, Elsa Arbajian   +10 more
wiley   +1 more source

Optimization of Oligonucleotide DNA Microarrays

, 2007
Optimization of oligonucleotide DNA microarrays is a multiparametric problem. The goal of the optimization process is to get conditions that capture target DNA with high sensitivity and selectivity. Parameters determining the performance of the microarray are spot morphology, probe and target density, background, and selectivity. More than 10 variables
Martin Dufva, Claus Christensen
openaire   +3 more sources

DNA Microarray Technology [PDF]

, 2002
Collaboration between Sandia National Laboratories and the University of New Mexico Biology Department resulted in the capability to train students in microarray techniques and the interpretation of data from microarray experiments. These studies provide for a better understanding of the role of stationary phase and the gene regulation involved in exit
WERNER-WASHBURNE, MARGARET, DAVIDSON, GEORGE S.   +1 more
openaire   +2 more sources

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source

Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array

BMC Genomics, 2011
Background Recent progress in high-throughput technologies has greatly contributed to the development of DNA methylation profiling. Although there are several reports that describe methylome detection of whole genome bisulfite sequencing, the high cost ...
Zhao Qian, He Ximiao, Dai Wei, Zhang Kunlin, Yang Liu, Wang Jing, Sun Zhong   +6 more
doaj   +1 more source

Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays [PDF]

, 2008
Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis.
Baranowski, Eric, Deplanche, Martine, François, Jean Marie, Leberre, Véronique, Trouilh, Lidwine   +4 more
core   +2 more sources

INF2‐Related Charcot–Marie–Tooth Disease in a Japanese Cohort: Genetic and Clinical Insights

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background INF2 mutations cause focal segmental glomerulosclerosis (FSGS) and Charcot–Marie–Tooth disease (CMT). Accurate genetic diagnosis is critical, as INF2‐related FSGS is typically resistant to immunotherapy yet rarely recurs after transplantation, and its associated neuropathy can mimic treatable immune‐mediated disorders such as ...
Chikashi Yano, Masahiro Ando, Yujiro Higuchi, Jun‐Hui Yuan, Akiko Yoshimura, Takahiro Hobara, Risa Nagatomo, Fumikazu Kojima, Yu Hiramatsu, Satoshi Nozuma, Tomonori Nakamura, Yusuke Sakiyama, Chika Matsuoka, Toru Yamashita, Takashi Kimura, Ayako Miyazaki, Chinatsu Kinjo, Kenji Yokochi, Nanami Yamanaka, Nozomu Matsuda, Tomoki Suichi, Yoshiyuki Hanaoka, Haruka Kojima, Kenichi Todo, Hiroyuki Ishiura, Jun Mitsui, Shoji Tsuji, Hiroshi Takashima   +27 more
wiley   +1 more source

An Overview of DNA Microarray Grid Alignment and Foreground Separation Approaches

EURASIP Journal on Advances in Signal Processing, 2006
This paper overviews DNA microarray grid alignment and foreground separation approaches. Microarray grid alignment and foreground separation are the basic processing steps of DNA microarray images that affect the quality of gene expression information ...
Bajcsy Peter
doaj   +1 more source

Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

BMC Genomics, 2003
Background The ADGE technique is a method designed to magnify the ratios of gene expression before detection. It improves the detection sensitivity to small change of gene expression and requires small amount of starting material. However, the throughput
Ile Kristina E, Davis Warren, Gaté Laurent, Chen Zhijian J, Tew Kenneth D   +4 more
doaj   +1 more source

Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis infection in vitro [PDF]

, 2013
BACKGROUND: Mycobacterium bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response.
Browne, JA, Conlon, KM, Gordon, SV, Gormley, E, Hokamp, K, Killick, KE, Loftus, BJ, Lohan, AJ, MacHugh, DE, Magee, DA, Nalpas, NC, Park, SD, Rue-Albrecht, K, Taraktsoglou, M   +13 more
core   +2 more sources